Phosl_00140 : CDS information

Location

Organism	Streptomyces sp.
Strain	HK803
Entry name	Phoslactomycin
Contig	AY354515
Start / Stop / Direction	36,297 / 37,508 / + [in whole cluster] 36,297 / 37,508 / + [in contig]
Location	36297..37508 [in whole cluster] 36297..37508 [in contig]
Type	CDS
Length	1,212 bp (403 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	PlmT4
Gene
Gene (GenBank)	plmT4
EC number
Keyword
Note
Note (GenBank)	cytochrome P450
Reference	ACC Q6V1N2 PmId [12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003) comment Phoslactomycins生合成gene clusterのクローニング、配列解析報告。 fig2: cytochrome P-450 monooxygenases plmS2, plmT4は、postpolyketide modificationに関連する酵素として図示されている。 C-8とC-8 ethyl側鎖でのhydroxylationは、plmT4によってコードされるP-450 monooxygenaseによっておそらく触媒される。 plm clusterにあるもうひとつのCYP450(plmS2)がC-18を触媒することが確認されたので、消去法でC-8での触媒を割り当てている。
Related Reference	ACC Q59831 PmId [7557483] Cloning, characterization and expression of the gene encoding cytochrome P-450sca-2 from Streptomyces carbophilus involved in production of pravastatin, a specific HMG-CoA reductase inhibitor. (Gene. , 1995) comment 15th, 43%, 3e-83 Streptomyces carbophilus_CYP105A3 Cytochrome P450(Cytochrome P450 sca-2) Pravastatin(高コレステロール血症薬)は、ML-236B-NaをStreptomyces carbophilusのcytochrome P-450sca (CytP-450sca) monooxygenase systemによって水酸化することによってつくられる(青カビ(Penicillium citrinum)を用いてML-236Bメバスタチンを生産し、これをStreptomyces carbophilusにより水酸化する二段階発酵法)。P-450sca (CytP-450sca) monooxygenase systemの遺伝子をクローニング、性質を調べている。

Sequence

>putative cytochrome P450 [PlmT4]
MSQLSSELPAFPMSKAKGCPLDPPPEYAQLRSDRPVAKARLWDGKEVWLITGYDEIRSIF
TDPRISVDNTQPGYPWLSEQARTVVLTGGVKPVGRMDPPEHTAMRRMLGQGFLVKKIQNM
RGDVEALVNELIDDILAGPRPTDLVPSLAMPVPSTALGWVLGVPPADKRLISLVPRLFDE
DSGLEGAMEARAELFAYIDELITHRENQPGDDIISHLVGYYQKGELSRVSVLTQSVTLIA
AALDTTRSMITNGILALLQHPEQAAALIEDPDLVPAAVEELLRYTVVTEFSSKRVAAADI
EIAGETIKAGDGIICLISAGNRDEKVFTDPDTLDVRRDAKQHLGFGAGIHTCIGKQLARM
ELEVVYGTLFRRIPELRLAVPFDQLVFRNTFDVQGVRALPVTW

>putative cytochrome P450 [PlmT4]
ATGTCACAGCTCTCGTCCGAACTCCCCGCTTTCCCCATGTCCAAGGCGAAGGGCTGCCCC
TTGGACCCGCCCCCCGAATACGCTCAGTTGCGGTCGGACCGGCCGGTCGCGAAAGCGAGG
CTGTGGGACGGCAAGGAAGTGTGGCTGATCACCGGGTATGACGAGATCCGCTCGATCTTC
ACCGACCCGCGTATCAGTGTCGACAACACGCAGCCCGGTTATCCCTGGCTGAGTGAGCAA
GCGCGGACCGTCGTGCTGACCGGCGGCGTGAAGCCGGTGGGCCGGATGGACCCGCCCGAG
CACACCGCCATGCGGCGCATGCTGGGCCAGGGCTTCCTGGTCAAGAAGATCCAGAACATG
CGCGGTGACGTGGAGGCCCTCGTCAACGAGCTCATCGACGACATTCTCGCGGGTCCTCGG
CCGACGGACCTGGTGCCGTCGTTGGCCATGCCGGTGCCGTCCACCGCACTGGGATGGGTG
CTGGGTGTCCCCCCAGCCGACAAGCGCCTCATCAGCCTGGTTCCCCGGCTGTTCGACGAG
GACTCCGGGCTGGAGGGCGCCATGGAGGCACGCGCCGAACTGTTCGCGTACATCGACGAG
TTGATCACGCACCGGGAGAACCAACCGGGCGACGACATCATCAGCCACCTGGTCGGCTAC
TACCAGAAGGGGGAGCTGTCGCGCGTCTCCGTGCTCACCCAGTCGGTCACGCTGATCGCG
GCGGCACTCGACACCACCAGGAGCATGATCACCAACGGGATCCTGGCCCTGCTGCAGCAT
CCCGAGCAGGCGGCGGCGCTGATCGAGGACCCGGACCTCGTACCGGCGGCGGTCGAGGAA
CTCCTGCGCTACACGGTCGTCACGGAGTTCTCGTCGAAGCGCGTGGCGGCCGCGGACATC
GAGATCGCGGGCGAGACCATCAAGGCGGGTGACGGCATCATCTGCCTGATCAGCGCGGGC
AACCGGGACGAGAAGGTCTTCACCGATCCCGACACCCTGGACGTCCGGCGGGACGCCAAA
CAGCACCTGGGCTTCGGGGCCGGCATCCACACGTGCATCGGGAAGCAACTGGCGCGCATG
GAGCTGGAAGTGGTGTACGGAACCCTGTTCCGCCGGATCCCCGAGCTGCGGCTGGCGGTC
CCCTTCGACCAGCTGGTCTTCCGCAACACGTTCGACGTGCAGGGCGTCCGGGCGCTTCCC
GTCACGTGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PR00385 P450 PF00067 p450 G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	No significant hit

Phosl_00130 Phosl_00150