Phosl_00190 Phosl_00190   Phosl_00210 Phosl_00210

Phosl_00200 : CDS information

close this sectionLocation

Organism
StrainHK803
Entry namePhoslactomycin
Contig
Start / Stop / Direction59,191 / 62,202 / + [in whole cluster]
59,191 / 62,202 / + [in contig]
Location59191..62202 [in whole cluster]
59191..62202 [in contig]
TypeCDS
Length3,012 bp (1,003 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productputative 3-phosphoshikimate 1-carboxyvinyltransferase/putative carboxylic acid--CoA ligase
Product (GenBank)PlmJK
Gene
Gene (GenBank)plmJK
EC number
Keyword
  • CHC
Note
  • bifunctional protein
Note (GenBank)
Reference
ACC
PmId
[12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003)
comment
Phoslactomycins生合成gene clusterのクローニング、配列解析報告。

S. collinusで同定されているCHC-CoA生合成遺伝子セットの保存がplmJK, plmL, chcA, plmMに見られる。

CHC-CoAはPKS starter unit.
CHC = cyclohexanecarboxylic acid
Related Reference
ACC
P0A6D3
PmId
[1899181] 5-Enolpyruvylshikimate-3-phosphate synthase from Escherichia coli--the substrate analogue bromopyruvate inactivates the enzyme by modifying Cys-408 and Lys-411. (Arch Biochem Biophys. , 1991)
comment
For N-term
blast list外, 30%, 2e-39
E.coli_aroA
3-phosphoshikimate 1-carboxyvinyltransferase(EC 2.5.1.19)
synonym: 5-enolpyruvylshikimate-3-phosphate synthase

HAMAP MF_00210 template
ACC
O07454
PmId
[9177244] A cluster of bacterial genes for anaerobic benzene ring biodegradation. (Proc Natl Acad Sci U S A. , 1997)
comment
For C-term
10th, 42%, 1e-101, C末部分hit
Rhodopseudomonas palustris_aliA
Cyclohex-1-ene-1-carboxylate CoA ligase(EC 6.2.1.-)
synonym: Cyclohexanecarboxylate-CoA ligase

大腸菌にクローニングし、酵素活性を見ている。
ACC
P38135
PmId
[12535077] A new Escherichia coli metabolic competency: growth on fatty acids by a novel anaerobic beta-oxidation pathway. (Mol Microbiol. , 2003)
[15213221] The Escherichia coli fadK (ydiD) gene encodes an anerobically regulated short chain acyl-CoA synthetase. (J Biol Chem. , 2004)
comment
For C-term
90th, 35%, 7e-77, C末部分hit
E.coli_fadK
Short-chain-fatty-acid--CoA ligase(EC 6.2.1.-)
synonym: Acyl-CoA synthetase

---
[PMID:12535077](2003)
ydiD mutantを作成し各条件下での生育を見ている。
ydiD geneはいくつかの判断基準に基づきacyl-CoA synthetase活性を持つタンパクをコードする候補とされているが、直接的な酵素解析はされていない。

ydiD → fadKへ改名している。

---
同じ著者らの続報
[PMID:15213221](2004)
鎖長によるFAの特異性を調査。FadKは短鎖FA基質(C<10)を好むacyl-CoA synthetaseであった。

また、His6-FadKによる
[1-14C]oleate → [1-14C]oleoyl-CoA
[1-14C]octanoate → [1-14C]octanoyl-CoA　
への変換を確認。この反応からCoAを除いてacyl-AMP中間体も検出している。
Family/Domain
FD
IPR006264
comment
[IPR006264] 3-phosphoshikimate 1-carboxyvinyltransferase　(Family)
function; 3-phosphoshikimate 1-carboxyvinyltransferase activity

close this sectionSequence

selected fasta
>putative 3-phosphoshikimate 1-carboxyvinyltransferase/putative carboxylic acid--CoA ligase [PlmJK]
MHLLVKGIQHRISSEILVPNSKYHAHRALILASLADGESRIHGLSDARHVEYTVRLLRGL
GVQLVREGDTFVVRGLGGHYRPVQDTVSAGSSGTTLYFMTGLASLSNRAVTITGQKYFRN
RPVGPLLRALEQIGVRLESANDCPPVQVRSHRPTGGEVTIPGTLSQWISGLLLLAPFATG
PTTITVSDTLNERTYLELTVAMMRQFGLEVTVAPDWRRFDVAPHQSVRPTELTLPPDIGS
AAFGIATAALHPSDVLLRGMPSLTGGPADHPEFHFLDIARSMGVPMELDPVAGGVRIQQD
APLLKAVDVDCRDVPDMLPVLSTLATFAHGESVFRNVAHTRLKESDRAGVMVQLNSMGAE
LELSDDTLRVTGVDSLVGAELSSYNDHRVLMSLAVASSRARGHSTLSYPHAYRISYPGFL
DMMNSVGVPTSVEKGPARSARSRARRPVREPVRSVADPDHACEVTLPEWLARHAASRPGD
TALVDVRPDGDSVVTWSDLAGRVDKAAALLLELGVRPGENVAYQLPNRLEFVVLSLAALR
IGAVCCPVIPFFRERELGFVLRRSLARVLVVMDEYRSRRPAQEALALAATGGPDTAHLEH
VVVLARSGGGSRPPQPPPGGVRLHDWESALRTTTVDRAALDAIRPTPGATAQLLFTSGTT
GEPKGVMQSSSNLVRAVSMEIRHLGLGPEDAVWVPSPLAHQTGFLYGMTLATVLGVPQIL
QSDWDARRALVSLNTHRATFVQAATPFLADLVKAVEEIGEAPRHLRLFVATGATVPRSLA
EHASEVLGTMVCGAFGTTETCLGALSRPTDTPERRWGTDGRVLDGVRLRVTDDRGAVLAP
GAEGNFEILSRTTFEGYLDRPDLTAEAFTPDGWFRTGDLAVLDESGYVRITGRVKDVINR
GGEKIPVGEMEQLLFRHPAVDDVAIVAMPDERLGERACAFVVLKDGALLGFEEMCRYLDG
HQAAKQYWPERLQLVPDLPRNPIGKVQKFELRARARHLRPHMQ
selected fasta
>putative 3-phosphoshikimate 1-carboxyvinyltransferase/putative carboxylic acid--CoA ligase [PlmJK]
ATGCATCTTCTCGTCAAGGGAATTCAGCACCGGATTTCCAGCGAAATCCTCGTACCGAAC
TCCAAATATCATGCACACCGCGCGCTGATCCTCGCCTCGCTCGCGGACGGGGAGAGCCGG
ATCCACGGCCTGTCCGACGCCCGGCACGTCGAGTACACCGTGCGGCTGCTGCGCGGCCTC
GGCGTACAGCTCGTCCGCGAGGGCGACACCTTCGTGGTGCGGGGGCTCGGCGGCCACTAC
CGGCCGGTCCAGGACACCGTCTCGGCCGGCAGCTCCGGCACCACCCTCTATTTCATGACC
GGGCTCGCGTCCCTGTCGAACCGCGCGGTCACGATCACCGGGCAGAAATACTTCCGTAAC
CGCCCCGTGGGCCCGCTGCTGCGCGCGCTGGAACAGATAGGTGTACGCCTGGAGTCGGCG
AATGACTGCCCGCCCGTCCAGGTCCGCAGCCATCGACCCACGGGCGGCGAGGTGACCATT
CCGGGCACCTTGTCCCAGTGGATATCCGGACTGCTGCTGCTCGCGCCGTTCGCCACCGGC
CCCACCACCATCACCGTGTCGGACACCCTCAACGAACGCACCTATCTGGAGCTGACCGTC
GCGATGATGCGGCAGTTCGGCCTGGAAGTGACCGTGGCGCCCGACTGGCGGCGCTTTGAC
GTGGCGCCGCATCAGAGCGTGCGGCCCACCGAGCTGACGTTGCCCCCCGACATCGGTTCC
GCGGCCTTCGGCATCGCCACCGCCGCGCTCCACCCCTCCGACGTGCTCCTGCGCGGTATG
CCGAGCCTGACCGGGGGGCCGGCCGACCACCCCGAGTTCCACTTCCTCGACATCGCACGC
TCGATGGGCGTCCCCATGGAACTGGACCCGGTGGCCGGCGGTGTCCGGATCCAGCAGGAC
GCCCCCCTGCTGAAGGCCGTCGATGTCGACTGCCGCGACGTGCCGGACATGCTTCCCGTC
CTTTCCACCCTCGCCACCTTCGCGCACGGCGAGTCGGTGTTCCGCAACGTCGCGCACACG
CGGCTGAAGGAGTCCGACCGGGCCGGCGTGATGGTCCAGCTCAACTCCATGGGCGCCGAG
CTGGAGTTGTCCGACGACACCCTGCGGGTGACCGGGGTGGACTCTCTGGTCGGCGCGGAG
CTGTCCTCGTACAACGACCACCGGGTGCTGATGTCGCTGGCCGTCGCCTCCTCGCGCGCC
CGGGGCCACTCCACGCTGTCGTACCCGCACGCCTACCGCATCTCGTATCCGGGCTTCCTC
GACATGATGAACAGCGTCGGCGTGCCGACCTCCGTGGAGAAGGGCCCCGCACGCTCCGCC
AGGAGCCGTGCCCGCCGGCCCGTCCGGGAACCCGTGCGGTCTGTCGCGGATCCGGACCAC
GCCTGCGAGGTCACCCTGCCGGAGTGGCTGGCCCGGCACGCCGCGTCCCGCCCCGGTGAC
ACCGCCCTCGTGGACGTGCGCCCGGACGGCGACTCCGTCGTCACCTGGAGCGACCTGGCC
GGCCGGGTGGACAAGGCCGCCGCGCTGCTGCTCGAACTCGGTGTGCGGCCGGGCGAGAAC
GTGGCGTACCAGCTGCCCAACCGCCTGGAGTTCGTGGTGCTGTCGCTGGCGGCGCTGCGG
ATCGGCGCCGTCTGCTGCCCGGTCATCCCGTTCTTCCGGGAACGCGAACTCGGCTTCGTC
CTGCGCCGCTCGCTGGCCCGCGTCCTGGTCGTCATGGACGAGTACCGGTCCCGGCGCCCC
GCGCAGGAGGCACTTGCCCTCGCCGCCACCGGTGGCCCGGACACGGCGCACCTGGAGCAT
GTCGTGGTGCTGGCCCGGTCGGGCGGCGGCAGCCGACCGCCACAGCCGCCCCCGGGCGGC
GTCCGCCTGCACGACTGGGAGAGCGCCCTGCGGACGACGACGGTCGACCGGGCGGCCCTC
GACGCGATCCGGCCGACGCCCGGGGCGACGGCGCAGCTGCTGTTCACCTCGGGCACCACC
GGCGAGCCCAAGGGGGTCATGCAGTCGTCCTCGAACCTGGTCCGGGCCGTGTCGATGGAG
ATCCGGCATCTCGGCCTCGGTCCCGAGGACGCCGTCTGGGTGCCCTCTCCGCTCGCCCAC
CAGACCGGGTTCCTGTACGGGATGACGCTGGCGACCGTGCTGGGTGTGCCCCAGATCCTG
CAGTCCGACTGGGACGCGCGGCGCGCCCTGGTCTCGCTGAACACGCACCGGGCGACGTTC
GTCCAGGCCGCCACGCCGTTCCTGGCCGATCTGGTGAAGGCGGTCGAGGAGATCGGCGAG
GCGCCCCGGCATCTGCGGCTCTTCGTGGCCACCGGCGCCACCGTGCCCCGCTCGCTGGCC
GAGCACGCGAGCGAGGTGTTGGGCACGATGGTGTGCGGCGCGTTCGGCACCACCGAAACC
TGCCTCGGCGCGCTGTCGAGGCCGACCGACACACCGGAGCGGCGCTGGGGCACCGACGGG
CGCGTCCTGGACGGCGTACGGCTGCGGGTCACCGACGATCGGGGCGCGGTGCTGGCGCCG
GGCGCCGAGGGCAACTTCGAGATCCTCTCGCGGACGACCTTCGAGGGCTATCTCGACCGC
CCCGACCTGACCGCGGAGGCCTTCACCCCGGACGGCTGGTTCCGCACCGGCGACCTCGCG
GTGCTCGACGAGTCGGGCTACGTCCGTATCACCGGCCGGGTCAAGGACGTGATCAACCGC
GGCGGCGAGAAGATCCCCGTCGGCGAGATGGAGCAGCTGCTGTTCCGCCACCCCGCCGTC
GACGACGTGGCCATCGTGGCCATGCCCGACGAGCGTCTCGGCGAGCGTGCCTGCGCGTTC
GTGGTGCTCAAGGATGGCGCGCTGCTCGGCTTCGAGGAGATGTGCCGCTACCTGGACGGG
CACCAGGCAGCCAAGCAGTACTGGCCGGAACGGCTCCAGCTCGTCCCGGATCTCCCGCGC
AACCCGATCGGCAAGGTCCAGAAGTTCGAACTGCGAGCCAGGGCGCGCCACCTGCGGCCC
CACATGCAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[495-924] 1.79999999999997e-87 PF00501
PF00501   AMP-binding
IPR001986 Enolpyruvate transferase domain (Domain)
[9-422] 4.49999999999996e-81 PF00275
PF00275   EPSP_synthase
[25-233] 5.50000000000008e-77 G3DSA:3.65.10.10 [234-428] 1.29999999999998e-83 G3DSA:3.65.10.10
G3DSA:3.65.10.10   EPSP_synthase
IPR006264 3-phosphoshikimate 1-carboxyvinyltransferase (Family)
[15-427] 4.59999999999996e-92 TIGR01356
TIGR01356   AroA
IPR013792 RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta (Domain)
[1-430] 8.10000734323267e-96 SSF55205
SSF55205   RNA3&apos;_cycl/enolpyr_transf_A/B
IPR020845 AMP-binding, conserved site (Conserved_site)
[653-664] PS00455
PS00455   AMP_BINDING
SignalP No significant hit
TMHMM No significant hit
Phosl_00190 Phosl_00190   Phosl_00210 Phosl_00210
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