Phosl_00210 : CDS information

Location

Organism	Streptomyces sp.
Strain	HK803
Entry name	Phoslactomycin
Contig	AY354515
Start / Stop / Direction	62,226 / 63,392 / + [in whole cluster] 62,226 / 63,392 / + [in contig]
Location	62226..63392 [in whole cluster] 62226..63392 [in contig]
Type	CDS
Length	1,167 bp (388 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	putative acyl-CoA dehydrogenase
Product (GenBank)	PlmL
Gene
Gene (GenBank)	plmL
EC number	1.3.99.-
Keyword	CHC
Note
Note (GenBank)
Reference	ACC Q6V1M5 PmId [12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003) comment Phoslactomycins生合成gene clusterのクローニング、配列解析報告。 S. collinusで同定されているCHC-CoA生合成遺伝子セットの保存がplmJK, plmL, chcA, plmMに見られる。 CHC-CoAはPKS starter unit. CHC = cyclohexanecarboxylic acid
Related Reference	ACC Q937T2 PmId [11571189] Cloning and genetic characterization of dca genes required for beta-oxidation of straight-chain dicarboxylic acids in Acinetobacter sp. strain ADP1. (Appl Environ Microbiol. , 2001) comment 471st, 33%, 1e-46 Acinetobacter sp._dcaA Acyl-CoA dehydrogenase beta酸化のstepに関連した3つの遺伝子のノックアウトは、Acinetobacter sp. strain ADP1染色体に導入された。mutantそれぞれはadipate(アジピン酸)で育つことができなかった。mutantsは付加的に飽和させられた直鎖ジカルボン酸を利用するための能力において影響されるので、新しく発見された10kbpDNAはdca(ジカルボン酸dicarboxylic acid)領域と名づけられた。 dcaA(acyl-CoA DH homolog) dcaE(enoyl-CoA hydratase homolog) dcaH(hydroxyacyl-CoA DH homolog)

Sequence

>putative acyl-CoA dehydrogenase [PlmL]
MMDEQRFGQVRDMVERWVEGPGERWAERIEETGEVPEAVWSELDELGFLRLAAPVEYGGQ
GLGFTQWMELMEIFSRSHGSLRMIVHVVNGIWRSMDGHACDEQRKRFVVPSVLGEIKIAF
ALTEPGNGTGADITTSVVREGDTYYLSGRKHLITFGMRCDYYLLAARVAGSAGHEGTVAL
LVPRNAPGVSAEDTSRTMGVTGTDHASIVFDRTPVPVDHRLGEEGQGLEVFLGGFLTPSR
ISVAMSCVGLAQRAQQLAVDYARSRVTFGKSLTERQAIQFMLAENAADIEAARQLVLHAA
RRFEEGADDASMQSSMAKMHAVTMLTTVTDKALQVHGGLGYWKSQKIERVYRDARAQRFE
EGPNEVQKAVVFRELLRQAGTVTAGGAR

>putative acyl-CoA dehydrogenase [PlmL]
ATGATGGACGAGCAGCGGTTCGGACAAGTCCGTGACATGGTCGAGCGGTGGGTGGAGGGC
CCGGGCGAGCGCTGGGCCGAACGCATCGAGGAGACCGGCGAGGTGCCCGAGGCGGTGTGG
AGCGAGCTCGACGAGCTCGGGTTCCTGCGGCTCGCCGCCCCGGTGGAGTACGGCGGCCAG
GGCCTCGGGTTCACCCAGTGGATGGAGCTGATGGAGATCTTCTCCCGCTCCCACGGTTCC
CTGCGCATGATCGTGCACGTCGTCAACGGCATCTGGCGGTCGATGGACGGTCACGCCTGC
GACGAACAGCGCAAGCGGTTCGTGGTGCCGTCCGTCCTGGGCGAGATCAAGATCGCGTTC
GCGCTGACCGAGCCGGGCAACGGCACCGGGGCGGACATCACGACCTCGGTGGTCCGCGAG
GGCGACACCTACTACCTGTCGGGCCGCAAGCACCTGATCACGTTCGGGATGCGCTGTGAC
TACTACCTCCTGGCGGCGCGCGTCGCGGGCTCCGCGGGCCACGAGGGCACCGTCGCCCTG
CTCGTGCCGCGCAACGCGCCCGGAGTGAGCGCCGAGGACACCTCCCGCACGATGGGCGTC
ACCGGCACCGACCACGCCTCGATCGTCTTCGACCGCACCCCCGTGCCCGTGGACCACCGG
CTCGGCGAGGAGGGCCAGGGGCTGGAGGTGTTCCTCGGGGGGTTCCTCACCCCGTCGCGG
ATCTCCGTCGCGATGAGCTGCGTCGGCCTCGCCCAGCGCGCCCAGCAGCTCGCCGTCGAC
TACGCCCGCTCCCGGGTGACCTTCGGCAAGTCGCTGACGGAGCGTCAGGCCATTCAGTTC
ATGCTCGCCGAGAACGCGGCGGACATCGAGGCGGCCCGACAGCTCGTGCTGCACGCCGCA
CGCCGCTTCGAGGAGGGCGCCGACGACGCGTCCATGCAGTCCTCGATGGCCAAGATGCAC
GCGGTGACGATGCTGACCACCGTCACCGACAAGGCGCTGCAGGTCCACGGCGGGCTCGGC
TACTGGAAGTCCCAGAAGATCGAGCGCGTCTACCGCGACGCCCGTGCCCAGCGCTTCGAG
GAGGGCCCCAACGAGGTGCAGAAAGCCGTGGTCTTCCGCGAACTGCTGCGGCAGGCCGGG
ACCGTCACCGCGGGAGGTGCGCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain) PF00441 Acyl-CoA_dh_1 IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain) G3DSA:2.40.110.10 Acyl_CoA_DH/ox_M PF02770 Acyl-CoA_dh_M IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain) PF02771 Acyl-CoA_dh_N IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain) G3DSA:1.20.140.10 AcylCoA_DH_1/2_C SSF47203 AcylCoADH_C_like IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain) SSF56645 AcylCoA_dehyd_NM IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain) G3DSA:1.10.540.10 AcylCoA_DH/ox_N
SignalP	No significant hit
TMHMM	No significant hit

Phosl_00200 Phosl_00220