Phosl_00220 : CDS information

Location

Organism	Streptomyces sp.
Strain	HK803
Entry name	Phoslactomycin
Contig	AY354515
Start / Stop / Direction	63,389 / 64,231 / + [in whole cluster] 63,389 / 64,231 / + [in contig]
Location	63389..64231 [in whole cluster] 63389..64231 [in contig]
Type	CDS
Length	843 bp (280 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	putative 1-cyclohexenylcarbonyl-CoA reductase
Product (GenBank)	ChcA
Gene
Gene (GenBank)	chcA
EC number
Keyword	CHC
Note
Note (GenBank)
Reference	ACC Q6V1M4 PmId [12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003) [16609856] Genetic manipulation of the biosynthetic process leading to phoslactomycins, potent protein phosphatase 2A inhibitors. (J Ind Microbiol Biotechnol. , 2006) comment [PMID: 12819191](2003) Phoslactomycins生合成gene clusterのクローニング、配列解析報告。 S. collinusで同定されているCHC-CoA生合成遺伝子セットの保存がplmJK, plmL, chcA, plmMに見られる。 CHC-CoAはPKS starter unit. CHC = cyclohexanecarboxylic acid --- [PMID: 16609856](2006) plmS2 deletion mutantであるNP2 mutantはphoslactomycin(PLM) Bのみを産生。この株のchcAをapramycin resistance geneで置換したNP3 mutantはPLM B非産生、CHC補充で回復。
Related Reference	ACC P95727 PmId [1597409] Purification and characterization of a novel enoyl coenzyme A reductase from Streptomyces collinus. (J Bacteriol. , 1992) [8955309] Cloning and characterization of the gene encoding 1-cyclohexenylcarbonyl coenzyme A reductase from Streptomyces collinus. (J Bacteriol. , 1996) [10973220] Identification of a cyclohexylcarbonyl CoA biosynthetic gene cluster and application in the production of doramectin. (Nat Biotechnol. , 2000) comment 2nd, 85%, 1e-135 Streptomyces collinus_chcA 1-cyclohexenylcarbonyl CoA reductase --- [PMID: 1597409](1992) 酵素を分離して活性測定。 --- [PMID: 8955309](1996) E.coliで異種性発現したS. collinus chcAを分離して活性測定。 vitroでcyclohexanecarboxylic acidの形成に関与する3つの還元ステップを触媒する。 S. collinus chcA deletion mutantは、ChcA activityと、cyclohexanecarboxylic acid または ansatrieninを合成する能力を失った。 --- [PMID: 10973220](2000) abstract S. collinusのansatrienin生合成gene clusterにおける5つのputative CHC-CoA生合成遺伝子を同定。

Sequence

>putative 1-cyclohexenylcarbonyl-CoA reductase [ChcA]
MNDIRHDDAAGGTKISLITGASRGIGRALALTLARQGGTVFVNYKKNAELAEKTVADIEE
AGGRGIAVQADVETTEGVTALFDEVGQRYGRLDHFVSNAAASVFKNILDLGQHHLDRSYA
MNLRPFVLGAQQAVKLMDDGGRIVALSSYGSVRAYPTYAALGGMKAAIEAWVRYMAVEFA
PYGINVNAVNGGLIDSDSLAYFYGIEGMPDMRGVLDRIPAGRPGTVQEMADTVAFLLGEG
AGYLTGQTLVVDGGLSVVAPPFFADAGEALRLPPRPTRES

>putative 1-cyclohexenylcarbonyl-CoA reductase [ChcA]
ATGAACGACATCCGACACGACGACGCGGCCGGCGGCACCAAGATCTCCCTCATCACAGGC
GCTTCGCGCGGCATCGGACGCGCCCTCGCCCTCACCCTGGCCCGCCAGGGCGGCACCGTG
TTCGTCAACTACAAGAAGAACGCCGAACTGGCCGAGAAGACCGTCGCCGACATCGAGGAG
GCCGGCGGCCGGGGCATCGCCGTGCAGGCCGATGTCGAGACGACGGAAGGCGTCACGGCG
CTGTTCGACGAGGTCGGACAGCGCTACGGACGCCTGGACCACTTCGTCTCCAACGCCGCC
GCCAGTGTCTTCAAGAACATCCTCGACCTCGGCCAGCACCACTTGGACCGCTCCTACGCC
ATGAACCTGCGCCCCTTCGTGCTCGGCGCCCAGCAGGCGGTCAAGCTCATGGACGACGGA
GGCCGGATCGTCGCGCTGTCGTCGTACGGATCGGTCCGCGCCTACCCGACGTACGCCGCG
CTCGGCGGGATGAAGGCGGCGATCGAGGCGTGGGTGCGGTACATGGCGGTCGAGTTCGCG
CCGTACGGCATCAACGTCAACGCGGTGAACGGCGGCCTGATCGATTCCGACTCGCTCGCC
TACTTCTACGGCATCGAGGGCATGCCCGACATGCGCGGCGTCCTGGACCGCATCCCCGCC
GGGCGGCCCGGCACGGTGCAGGAGATGGCCGACACCGTCGCCTTCCTGCTGGGTGAGGGG
GCCGGCTACCTCACGGGTCAGACCCTGGTCGTCGACGGGGGCCTGAGCGTGGTCGCCCCG
CCGTTCTTCGCGGACGCCGGCGAGGCACTGCGTCTGCCGCCCCGCCCGACACGGGAATCC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002347 Glucose/ribitol dehydrogenase (Family) PR00081 GDHRDH IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Phosl_00210 Phosl_00230