Phosl_00250 : CDS information

Location

Organism	Streptomyces sp.
Strain	HK803
Entry name	Phoslactomycin
Contig	AY354515
Start / Stop / Direction	70,996 / 72,189 / + [in whole cluster] 70,996 / 72,189 / + [in contig]
Location	70996..72189 [in whole cluster] 70996..72189 [in contig]
Type	CDS
Length	1,194 bp (397 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	putative phospho-2-dehydro-3-deoxyheptonate aldolase putative 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase putative DAHP synthase
Product (GenBank)	PlmI
Gene
Gene (GenBank)	plmI
EC number	2.5.1.54
Keyword
Note
Note (GenBank)	DAHP synthase
Reference	ACC Q6V1M1 PmId [12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003) comment Phoslactomycins生合成gene clusterのクローニング、配列解析報告。 S. collinusのCHC-CoA生合成gene cluster由来のchcA geneをプローブとして解析。 CHC-CoA starter unitの形成を担う3-deoxy-D-arabino-heptulosonate-7-phosphate synthaseをコードするplmIは高く保存されている。
Related Reference	ACC P80574 PmId [8760910] Evidence for a novel class of microbial 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase in Streptomyces coelicolor A3(2), Streptomyces rimosus and Neurospora crassa. (Microbiology. , 1996) comment 76th, 41%, 1e-71 Streptomyces coelicolor_aroH Phospho-2-dehydro-3-deoxyheptonate aldolase(EC 2.5.1.54) S. coelicolor A3(2)とS. rimosusのtryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate(DAHP) synthasesを精製し、活性測定。 N末の配列も決定している。配列の相同性からDAHPには2つのクラスがあると考えられている。
Related Reference	ACC Q6TZ03 PmId [14600244] ( , ) comment 77th, 40%, 1e-71 Amycolatopsis methanolica_aroG DAHP-synthase DAHP-synthaseを2つ(aroG,aroF)もっている。それぞれを、発現させ、活性をみたり、何により阻害されるかを確認している。 aroG(DS1:a plant-type DAHP synthase[Q6TZ03]) aroF(DS2:E. coli-type DAHP synthase[Q6TZ04]) aroGはchorismate mutase(EC5.4.99.5, CM)とcomplexとして機能する。この複合体タンパクの複合体のCM活性はTyr and Pheによって、DS1活性は主にTrpによってfeedback抑制される。

Sequence

>putative phospho-2-dehydro-3-deoxyheptonate aldolase [PlmI]
MVGSSVTQTRNPARQQPEWADPFQAERVRTILAERPPLVRAEDVRGLGDQLAGVARGEAH
VLQAGDCAEHPDECTAGHVSRKSALLHLLGDTLQAATGKPVVRVGRIAGQFAKPRSHRVE
PVDGVDLPVYRGHMVNAPTRDLAARRHDPLRMLMAYRAAHDVMEHLGWRGPAGDSHRPGT
GTSIPTWTSHEALVLDYEFPLVRDLDDGQRLLGSTHWPWIGKRTHQLDGAHIDLISEVLN
PVAVKVGPTTTPADLTALCARLDPQRRPGRLTLIARMGADAARSCLAPLVRTVHRTGHPV
IWLCDPMHGNTVTAPDGHKTRLLPTMAEEVQAFCRAVTFAGGIAGGLHLEATPDDVTECA
PDFHALDSPTVSRTTLCDPRLNPEQAVSLVSRWRQDP

>putative phospho-2-dehydro-3-deoxyheptonate aldolase [PlmI]
GTGGTGGGTAGCTCCGTCACGCAGACACGCAATCCCGCCAGGCAGCAGCCCGAATGGGCC
GACCCCTTCCAGGCGGAGCGGGTACGGACGATCCTCGCCGAGCGGCCTCCTCTGGTGAGG
GCTGAGGACGTCCGCGGACTCGGTGACCAGTTGGCGGGGGTGGCGCGAGGAGAGGCCCAC
GTCCTCCAGGCCGGTGACTGCGCGGAGCACCCCGACGAGTGCACGGCGGGACATGTCTCG
CGCAAGTCCGCCCTGCTTCACCTGCTCGGCGACACCCTGCAGGCCGCGACCGGCAAACCG
GTTGTGCGCGTGGGGCGCATCGCCGGCCAGTTCGCCAAGCCCCGCTCGCACCGGGTCGAA
CCGGTCGATGGTGTCGACCTGCCCGTCTATCGCGGACACATGGTCAACGCCCCCACTCGC
GACCTCGCCGCCCGCCGCCACGACCCCCTGCGGATGCTGATGGCCTACCGGGCGGCCCAC
GACGTCATGGAGCATCTCGGCTGGCGCGGCCCCGCCGGCGACTCACACCGCCCCGGTACC
GGTACCTCGATTCCGACCTGGACCAGCCACGAAGCACTTGTCCTGGACTACGAATTCCCC
CTGGTCCGTGACCTGGACGACGGACAACGTCTTCTCGGCTCCACCCATTGGCCCTGGATC
GGCAAGCGCACCCATCAGCTCGACGGTGCCCATATCGACCTGATCTCCGAGGTGCTCAAC
CCCGTTGCGGTGAAGGTCGGTCCCACGACCACCCCGGCCGACCTCACCGCGCTGTGTGCC
CGCCTCGACCCACAGCGCCGTCCGGGCCGCCTCACGCTCATCGCCCGCATGGGGGCCGAT
GCGGCACGCTCCTGCCTCGCCCCGCTCGTCCGGACCGTCCACCGTACCGGCCACCCGGTG
ATCTGGCTGTGCGACCCCATGCACGGCAACACCGTCACCGCCCCCGACGGCCACAAGACC
CGCTTGCTGCCCACCATGGCGGAGGAAGTGCAGGCCTTCTGCCGGGCCGTCACCTTCGCC
GGTGGCATCGCCGGCGGACTCCACCTGGAGGCCACACCCGACGACGTCACCGAATGCGCC
CCCGACTTCCACGCCCTCGACTCCCCCACCGTCAGCCGTACCACCCTGTGCGACCCCCGC
CTCAACCCTGAACAGGCCGTCTCACTCGTCTCCCGGTGGCGCCAAGACCCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002480 DAHP synthetase, class II (Family) PF01474 DAHP_synth_2
SignalP	Eukaryota Bacteria, Gram-negative
TMHMM	No significant hit

Phosl_00240 Phosl_00260