Phosl_00260 : CDS information

Location

Organism	Streptomyces sp.
Strain	HK803
Entry name	Phoslactomycin
Contig	AY354515
Start / Stop / Direction	73,871 / 72,672 / - [in whole cluster] 73,871 / 72,672 / - [in contig]
Location	complement(72672..73871) [in whole cluster] complement(72672..73871) [in contig]
Type	CDS
Length	1,200 bp (399 aa)

Annotation

Category	3.3 modification reduction
Product	cytochrome P450
Product (GenBank)	PlmS2
Gene
Gene (GenBank)	plmS2
EC number
Keyword	C-18 hydroxylate
Note
Note (GenBank)	cytochrome P450
Reference	ACC Q6V1M0 PmId [12819191] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster. (J Biol Chem. , 2003) [16291670] The plmS2-encoded cytochrome P450 monooxygenase mediates hydroxylation of phoslactomycin B in Streptomyces sp. strain HK803. (J Bacteriol. , 2005) [16609856] Genetic manipulation of the biosynthetic process leading to phoslactomycins, potent protein phosphatase 2A inhibitors. (J Ind Microbiol Biotechnol. , 2006) comment [PMID: 12819191](2003) Phoslactomycins生合成gene clusterのクローニング、配列解析報告。 plmS2のallelic replacementによるmutantはphoslactomycin(PLM)-Bのみを産生し、wildより6倍高い力価で産生される。 --- [PMID: 16291670](2005) [PMID: 16609856](2006) plmS2 deletion mutant NP2はPLM Bしか産生できない。 promoter下においたplmS2(pMSG1)で相補すると、Fを通してPLM A and PLM Cを産生できる。 S. coelicolor M511でPlmS2を発現、精製し、PLM B→PLM G(PLM BのC-18をhydroxylationしたもの)への変換をHPLCで測定。 PlmS2はPLM Bのために高く特異的であることも確認。

Sequence

>cytochrome P450 [PlmS2]
MVTVDLSAYGPGFFTDPYPYYARLREAGPVHEIVLADGDRFWLIVGYDEARAALADPRLA
KSLDPPSEDERHVLITDPPDHTRLRRLVSREFTARRVEAMRPRVQEITDGLLDEMVAGRR
RADLVPSLGSPLPITVLCELLGVPLADREDFRGWTERVLVPAEPDTIAWWKSRGFAQAGM
ALTDYLKNMIEDKRRSTPTGDLISSLLRTTAEDNDRLSAAELHSMVFILIVAGHETTANL
ITNGVRALLAHPEQLAALRTDPEGLIDQAVEEMLRYDGPVETSTKRFTLEAVRYGATKIP
PGETLLVSIAATGRDPAQFERPDTFDIHRGTTGTRSGHVAFGHGIHFCLGAGLARMESRV
AILTLLRRCPDLALDIDPAGLDWLPGIRVRGVRSLPVRW

>cytochrome P450 [PlmS2]
ATGGTGACCGTCGACCTGAGCGCGTACGGCCCCGGTTTCTTCACCGACCCGTACCCCTAC
TACGCCCGGCTGCGCGAGGCCGGGCCGGTGCACGAGATCGTCTTGGCCGACGGGGACAGG
TTCTGGCTGATCGTCGGCTACGACGAGGCCCGGGCGGCCCTGGCCGATCCACGTCTGGCC
AAGTCCCTCGACCCGCCGAGCGAGGACGAGCGGCACGTCCTGATCACCGATCCGCCGGAC
CACACGCGGCTGCGACGGCTCGTCTCGCGGGAGTTCACCGCGCGCCGGGTGGAGGCGATG
CGCCCGCGCGTTCAGGAGATCACCGACGGTCTCCTCGATGAGATGGTGGCCGGGCGGCGG
CGAGCCGACCTGGTCCCGTCGCTGGGGTCCCCGCTGCCGATCACCGTGCTCTGCGAACTG
CTCGGGGTCCCGCTCGCCGACCGGGAGGACTTCCGCGGCTGGACGGAGCGGGTTCTGGTG
CCGGCCGAACCGGACACCATAGCCTGGTGGAAGAGCCGGGGATTCGCGCAGGCCGGGATG
GCCCTGACGGACTACCTGAAGAACATGATCGAGGACAAGCGCCGCTCTACGCCGACCGGC
GACCTGATCTCCTCGCTGTTGCGGACAACCGCGGAGGACAACGACCGCCTGTCGGCCGCC
GAGCTGCACTCGATGGTGTTCATCCTGATCGTTGCGGGCCACGAGACCACGGCCAACCTG
ATCACCAACGGCGTACGCGCGCTCCTGGCACACCCCGAACAACTGGCGGCGCTGCGTACC
GACCCGGAGGGACTGATCGACCAGGCGGTCGAGGAGATGCTGCGCTACGACGGCCCGGTG
GAGACGTCGACCAAGCGGTTCACCCTCGAGGCCGTCAGATACGGCGCCACGAAGATCCCG
CCAGGGGAGACGCTTCTGGTCAGCATCGCGGCGACGGGGCGTGACCCTGCGCAGTTCGAG
CGACCCGACACGTTCGACATCCACCGCGGTACGACCGGCACCCGCAGTGGCCACGTCGCC
TTCGGCCACGGCATCCACTTCTGCCTGGGTGCCGGGCTGGCACGGATGGAGAGCCGGGTC
GCGATCCTGACCCTGCTGCGCCGCTGTCCCGACCTGGCGCTCGACATCGATCCGGCCGGT
CTCGACTGGCTGCCGGGCATCAGAGTCCGGGGTGTACGCAGTCTGCCGGTGCGCTGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 SSF48264 Cytochrome_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Phosl_00250 Phosl_00270