Pimar_00010 : CDS information

Location

Organism	Streptomyces natalensis
Strain	ATCC 27448 (=NBRC 13367)
Entry name	Pimaricin
Contig	FM864219
Start / Stop / Direction	819 / 175 / - [in whole cluster] 819 / 175 / - [in contig]
Location	complement(175..819) [in whole cluster] complement(175..819) [in contig]
Type	CDS
Length	645 bp (214 aa)

Annotation

Category	4.3 transport
Product	autoinducer exporter amino acid exporter
Product (GenBank)	PimT protein
Gene
Gene (GenBank)	pimT
EC number
Keyword
Note
Note (GenBank)
Reference	ACC C6GYU4 PmId [19505319] PimT, an amino acid exporter controls polyene production via secretion of the quorum sensing pimaricin-inducer PI-factor in Streptomyces natalensis. (Microb Cell Fact. , 2009) comment pimaricin gene clusterに隣接するRhtB family amino-acid exporter PimTについての論文。 pimTのgene replacement mutantは、L-homoserine, L-serine, and L-homoserine lactoneへの感受性増加。相補で回復。 L-aspartic acid, L-methionine, L-threonine, L-lysine, DL-hydroxynorvaline, D-homoserine, gamma-butyrolactoneへの感受性には変化なし。 pimT発現はhomoserineとquorum-sensing pimaricin-inducer PI-factor(autoinducer)によって誘導される。 pimT deletion mutantはpimaricin産生を65%、PI産生を50%まで減らし、pimT相補、外因性PI-factorの添加でpimaricin産生は回復する。これはPimTがPI-factor exporterとして使用されることを示す。以上から、pimaricin産生はamino acid exporter PimTによるquorum sensing pimaricin-inducer PI-factorの分泌を介して調節されている。

Sequence

>autoinducer exporter [PimT protein]
MVSTESILAFAAMSLLVIVIPGPSVLFVIGRALAHGRRTALATVVGNLIGSYVLVVAVAW
GLGALVETSAAVLMGVKLAGAVYLVYLGVQAFRHRKEMRAADMEAPAGERRGDLRTVLDG
IFVGVTNPKGIVFFAAVLPQFVDHSTGHVPLQMMVLGLVPVTIGMITDTLWGLGASAARS
WFARSDRRLSMVGGAGGFAMIGLGVTVAATGRAD

>autoinducer exporter [PimT protein]
ATGGTGTCCACGGAGAGCATTCTCGCGTTCGCGGCGATGTCGCTCTTGGTGATCGTGATA
CCGGGGCCGAGCGTGCTGTTCGTGATCGGCAGGGCCCTCGCGCACGGCCGCCGCACGGCA
CTCGCCACGGTCGTCGGCAACCTCATCGGCTCATACGTCCTGGTTGTCGCTGTGGCGTGG
GGACTTGGGGCGCTGGTGGAGACCTCGGCTGCGGTGCTCATGGGTGTGAAGCTGGCCGGA
GCGGTGTATCTCGTCTACCTCGGCGTGCAGGCATTCCGGCACCGCAAGGAGATGCGTGCA
GCGGACATGGAGGCACCGGCGGGCGAGCGGCGCGGCGATCTGCGCACGGTCCTGGACGGC
ATTTTCGTAGGCGTCACGAACCCGAAGGGCATCGTCTTCTTCGCGGCGGTGCTGCCGCAG
TTCGTGGACCACTCCACGGGTCATGTCCCGCTCCAGATGATGGTGTTGGGCCTGGTCCCG
GTCACCATCGGCATGATCACCGACACTCTGTGGGGCCTGGGCGCCTCGGCGGCCCGGTCC
TGGTTCGCCCGCTCGGACCGCCGCCTTTCGATGGTCGGCGGGGCGGGCGGCTTCGCGATG
ATCGGGCTGGGCGTGACGGTCGCGGCGACGGGCCGCGCCGACTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001123 Lysine-type exporter protein (LYSE/YGGA) (Family) PF01810 LysE
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative Eukaryota
TMHMM	Transmembrane 6

first Pimar_00020