Pimar_00080 : CDS information

Location

Organism	Streptomyces natalensis
Strain	ATCC 27448 (=NBRC 13367)
Entry name	Pimaricin
Contig	AJ278573
Start / Stop / Direction	51,435 / 50,668 / - [in whole cluster] 44,691 / 43,924 / - [in contig]
Location	complement(50668..51435) [in whole cluster] complement(43924..44691) [in contig]
Type	CDS
Length	768 bp (255 aa)

Annotation

Category	1.4 Other
Product	putative type II thioesterase
Product (GenBank)	PimI protein
Gene
Gene (GenBank)	pimI
EC number
Keyword	type II thioesterase
Note
Note (GenBank)
Reference	ACC Q9EWA0 PmId [11094342] A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis. (Chem Biol. , 2000) comment pimaricin biosynthetic gene clusterの報告。 PimI: TE
Related Reference	ACC Q6W5Q5 NITE FR008_00110 PmId [18836004] Selective removal of aberrant extender units by a type II thioesterase for efficient FR-008/candicidin biosynthesis in Streptomyces sp. strain FR-008. (Appl Environ Microbiol. , 2008) comment 22nd(Q6W5Q5) 60%, 2e-69 Streptomyces sp. FR-008_fscTE [FR008_00110]type II thioesterase fscTEはputative type II thioesterase (TEII)をコードする。 fscTE deletionでFR-008/candicidin産生は約90%減。 type I thioesteraseを不活化させたとき、FscTEは成熟伸長したpolyketideの放出を代償することができなかった。 FscTE and TylOのHydrolytic activitiesは、 propionyl-S-N-acetylcysteamine > methylmalonyl-S-N-acetylcysteamine acetyl-S-N-acetylcysteamine > malonyl-S-N-acetylcysteamine したがって、TEII はacyl carrier proteins(ACP)に結合するnonelongatable residuesを選択的に除去することにより、効果的なpolyketide生合成を維持することが結論付けられる。

Sequence

>putative type II thioesterase [PimI protein]
MTTTTAEGLWARSYHPAPHHRRQLVCFPHAGGSASFFFPVSAQLSSVAEVLSVQYPGRQD
RRTESSPSGIEAMADQVYAALQGRLRSRPTAFFGHSMGAMVAFEVARRLEADGGELTHLF
ASGRRAPSRYRAESVHRRDDDGIVAELKLLSGTDAALLGDEELLRMILPAIRSDYTAVET
YRCDPGAAIKAPITALTGDDDPKTTLEEAEGWRAHTTGAFDLRVYPGGHFFLSSQAQAVL
ALLREHLTAAEEFAV

>putative type II thioesterase [PimI protein]
ATGACGACGACCACGGCGGAAGGGCTCTGGGCCCGGAGCTACCACCCGGCGCCGCACCAT
CGACGCCAGCTCGTCTGCTTTCCGCACGCGGGCGGTTCGGCCTCTTTCTTCTTCCCCGTA
TCGGCACAGCTGTCATCCGTGGCGGAGGTGCTGTCGGTGCAGTACCCCGGCCGCCAGGAC
CGGCGCACCGAGTCCAGCCCCAGCGGCATCGAGGCCATGGCCGACCAGGTGTACGCGGCC
CTGCAAGGCCGGCTCCGGTCGCGGCCGACGGCGTTCTTCGGGCACAGCATGGGCGCGATG
GTCGCCTTCGAGGTGGCCCGGCGGCTGGAGGCCGACGGAGGCGAGCTGACGCACCTCTTC
GCTTCCGGCCGGCGCGCACCCTCGCGCTACCGCGCGGAGAGCGTGCACCGGCGTGACGAC
GACGGGATCGTCGCCGAACTGAAGCTGCTCTCCGGTACGGACGCCGCGCTGCTGGGCGAC
GAGGAGCTGCTGCGCATGATCCTCCCGGCGATCCGCAGCGACTACACCGCGGTGGAGACC
TACCGCTGCGATCCCGGAGCGGCGATCAAGGCGCCGATCACCGCGTTGACCGGTGACGAC
GACCCCAAGACCACGCTGGAGGAGGCCGAGGGGTGGCGCGCGCACACCACGGGGGCGTTC
GACCTCCGGGTCTACCCCGGCGGTCACTTCTTCCTCAGCTCCCAGGCCCAGGCCGTACTG
GCACTGCTGCGGGAACACCTCACCGCCGCCGAGGAGTTCGCCGTGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001031 Thioesterase (Domain) PF00975 Thioesterase
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Pimar_00070 Pimar_00090