Pimar_00090 Pimar_00090   Pimar_00110 Pimar_00110

Pimar_00100 : CDS information

close this sectionLocation

Organism
StrainATCC 27448 (=NBRC 13367)
Entry namePimaricin
Contig
Start / Stop / Direction53,027 / 54,835 / + [in whole cluster]
46,283 / 48,091 / + [in contig]
Location53027..54835 [in whole cluster]
46283..48091 [in contig]
TypeCDS
Length1,809 bp (602 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative ABC transporter ATP-binding/permease protein
Product (GenBank)PimA protein
Gene
Gene (GenBank)pimA
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11094342] A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis. (Chem Biol. , 2000)
comment
pimaricin biosynthetic gene clusterの報告。
PimA: ABC transporter

PimA and PimBは、いくつかのTMSsを持ち、ATP-dependent ABC transporter superfamilyに属するタンパクにマッチする。
PimAが相同性を示すのは、
ショウジョウバエのmultidrug resistance pump(Q00449)
E.coliのhaemolysinのpermeases(P08717) C末
Bordetella pertussisのcyclolysinのpermeases(P19770) C末
Related Reference
ACC
Q9L4X5
NITE
Nysta_00030
PmId
[16251298] Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin. (Antimicrob Agents Chemother. , 2005)
comment
21st(Q9L4X5) 66%, 1e-179
Streptomyces noursei_nysH
[Nysta_00030]ABC transporter

nysG, nysHを不活化してもnystatinは細胞外へ運ばれるので、他にも代替的なtransporterがあるようだ。不活化すると10- deoxynystatinの産生量が増えるので、C-10 hydroxylation(NysL)の効率に関係がある。ATPase inhibitor sodium o-vanadateを使った実験で産生量が減少する。domainについても検討し、明らかにtype III ABC transportersに属するといっている。

close this sectionSequence

selected fasta
>putative ABC transporter ATP-binding/permease protein [PimA protein]
MLLCLLRIHLRPHRRSVALLGLLQLVQILATLALPTLGAAVIDNGVVRADSGYITRTGLA
MLAVALVQIAASVAAVALGARTAMAMGRDLRSAVFRRVLDFSAREVGRFGTPSLMTRTVN
DVQQVQVLALSAFGVVVSAPLMCLGSIALALQQDVPLSLLLVALMVAVGMSFGLILGRTD
PFYARMQKQLDRINGLLRERITGVRVVRAFVRDAHEGARFGRTNSELRDISLRVGRLLAT
VIPLVLLVLNAFMAAVVWFGAHRIDAGAMRFGALSAFLSYLTLITMSVVMVTFVCLPMPR
ARVSAERIQQVLATEPSVAAPSEPYARCPQPGQLSLYGAGFRYPGAKEPVLHGIDLSVRP
GETVAILGSTGSGKTTLLNLVLRHFDATDGAVHVGGVDVRTLDAAVLRRTVGFVPQRPYL
FSGTVASNLRFGDPDATDDELWHALEIAQARDFVRLLPGGLNAPLTQGGTNVSGGQRQRL
AIARTLLRRPDIYLFDDCFSALDHATDAALRAALEPEISRATVVTVAQRVSTVRRAGRIV
VLDAGRVAATGTHEELLRTSTTYREIARSQLTAITGDHDGTGTDAAAATTLQEAVHGLDG
QL
selected fasta
>putative ABC transporter ATP-binding/permease protein [PimA protein]
GTGCTGCTATGTCTTCTGCGAATCCATCTGCGGCCGCACCGGCGCTCCGTCGCCCTGCTG
GGGCTTTTGCAACTGGTGCAGATCCTGGCCACTTTGGCCCTGCCGACACTGGGCGCCGCG
GTCATCGACAACGGCGTGGTCAGGGCCGACAGCGGCTACATCACCCGGACCGGCCTGGCC
ATGCTGGCCGTGGCGCTCGTGCAGATCGCGGCGTCCGTGGCCGCGGTGGCGCTGGGCGCC
CGTACGGCCATGGCGATGGGCCGCGACCTGCGCTCGGCCGTCTTCCGCCGGGTGCTGGAC
TTCTCGGCCCGCGAGGTCGGGCGGTTCGGCACTCCGTCGCTGATGACGCGGACCGTCAAC
GATGTGCAGCAGGTGCAGGTGCTGGCCCTGTCCGCGTTCGGCGTCGTCGTGTCGGCGCCC
CTGATGTGTCTGGGCAGCATCGCGCTCGCACTCCAGCAGGACGTCCCGCTCTCCCTGCTC
CTGGTGGCGCTGATGGTGGCCGTCGGAATGTCCTTCGGCCTCATTCTCGGCCGCACCGAT
CCGTTCTACGCCCGTATGCAGAAACAGCTGGACCGCATCAACGGGCTGCTGCGCGAACGC
ATTACCGGAGTCCGCGTCGTACGGGCTTTTGTGCGCGACGCCCACGAAGGCGCGAGATTC
GGCCGCACCAATTCCGAATTGCGTGACATCTCGCTGCGCGTCGGCCGGCTGCTGGCCACG
GTCATCCCCCTGGTGCTGCTGGTCCTCAACGCCTTCATGGCAGCCGTGGTGTGGTTCGGC
GCCCACCGCATCGACGCCGGGGCGATGCGGTTCGGTGCGCTCAGCGCGTTTCTGAGCTAC
CTGACGCTGATCACGATGTCGGTGGTGATGGTGACCTTCGTGTGCCTGCCGATGCCGCGG
GCGAGGGTTTCGGCCGAGCGGATCCAGCAGGTGCTGGCGACCGAGCCGAGCGTGGCGGCG
CCGAGTGAGCCGTACGCGCGCTGCCCGCAGCCGGGCCAGCTGTCCTTGTACGGAGCCGGG
TTCCGCTATCCGGGAGCGAAGGAGCCGGTGCTGCACGGCATCGATCTGAGCGTCCGGCCC
GGCGAGACGGTGGCGATCCTCGGCAGCACCGGCAGCGGCAAGACCACCCTGCTCAACCTC
GTGCTGCGGCACTTCGACGCCACCGACGGCGCCGTCCACGTGGGCGGCGTCGATGTCCGC
ACCCTGGACGCGGCGGTGCTGCGCCGCACCGTCGGCTTCGTACCGCAGCGGCCGTACCTC
TTCTCCGGCACCGTCGCGAGCAATCTGCGCTTCGGCGACCCGGACGCCACCGACGACGAA
CTCTGGCACGCGCTGGAGATCGCCCAGGCGCGCGATTTCGTGCGGCTCCTGCCCGGCGGG
CTGAACGCACCCCTCACCCAGGGCGGCACCAATGTGTCGGGCGGGCAACGGCAGCGCCTG
GCGATCGCCCGCACCCTGCTGCGCAGGCCGGACATCTATCTCTTCGACGACTGCTTCTCC
GCGCTGGACCACGCCACGGACGCCGCGCTGCGGGCCGCCCTGGAGCCGGAGATCTCCCGG
GCGACGGTGGTCACCGTCGCCCAGCGCGTCAGCACCGTCCGCCGCGCGGGGCGCATCGTC
GTCCTGGACGCGGGGCGGGTGGCCGCCACCGGAACGCACGAGGAGCTGCTGCGCACCAGT
ACGACGTACCGGGAGATCGCCCGGTCCCAGCTCACCGCGATCACCGGTGACCACGACGGC
ACCGGCACGGACGCCGCAGCCGCCACGACCCTTCAGGAGGCTGTCCATGGTCTCGACGGA
CAGCTCTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001140 ABC transporter, transmembrane domain (Domain)
[19-288] 4.70000000000001e-32 PF00664
PF00664   ABC_membrane
IPR003439 ABC transporter-like (Domain)
[375-499] 5.80000000000004e-18 PF00005
PF00005   ABC_tran
[334-569] PS50893
PS50893   ABC_TRANSPORTER_2
IPR003593 AAA+ ATPase domain (Domain)
[360-546] 5.8999880940569e-18 SM00382
SM00382   AAA
IPR011527 ABC transporter, transmembrane domain, type 1 (Domain)
[6-316] 2.99998750445706e-60 SSF90123
SSF90123   ABC_TM_1
IPR017871 ABC transporter, conserved site (Conserved_site)
[472-486] PS00211
PS00211   ABC_TRANSPORTER_1
IPR017940 ABC transporter, integral membrane type 1 (Domain)
[18-294] PS50929
PS50929   ABC_TM1F
SignalP
[1-33] 0.729 Signal
Eukaryota   
[1-39] 0.993 Signal
Bacteria, Gram-positive   
[1-33] 0.663 Signal
Bacteria, Gram-negative   
TMHMM
[20-42] Transmembrane (i-o) [57-79] Transmembrane (o-i) [127-149] Transmembrane (i-o) [155-177] Transmembrane (o-i) [237-259] Transmembrane (i-o) [274-296] Transmembrane (o-i)
Transmembrane 6   
Pimar_00090 Pimar_00090   Pimar_00110 Pimar_00110
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