Pimar_00100 Pimar_00100   Pimar_00120 Pimar_00120

Pimar_00110 : CDS information

close this sectionLocation

Organism
StrainATCC 27448 (=NBRC 13367)
Entry namePimaricin
Contig
Start / Stop / Direction54,813 / 56,693 / + [in whole cluster]
48,069 / 49,949 / + [in contig]
Location54813..56693 [in whole cluster]
48069..49949 [in contig]
TypeCDS
Length1,881 bp (626 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative ABC transporter ATP-binding/permease protein
Product (GenBank)PimB protein
Gene
Gene (GenBank)pimB
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11094342] A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis. (Chem Biol. , 2000)
comment
pimaricin biosynthetic gene clusterの報告。
PimB: ABC transporter

PimA and PimBは、いくつかのTMSsを持ち、ATP-dependent ABC transporter superfamilyに属するタンパクにマッチする。
PimBは、PimAが相同性を示すものと同じ種類のpermeasesに、より高い相同性がある。PimBとPimAはid28%。
Related Reference
ACC
Q9L4X6
NITE
Nysta_00020
PmId
[16251298] Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin. (Antimicrob Agents Chemother. , 2005)
comment
2nd(Q9L4X6) 71%, 0.0
Streptomyces noursei_nysG
[Nysta_00020]ABC transporter

nysG, nysHを不活化してもnystatinは細胞外へ運ばれるので、他にも代替的なtransporterがあるようだ。不活化すると10-deoxynystatinの産生量が増えるので、C-10 hydroxylation(NysL)の効率に関係がある。ATPase inhibitor sodium o-vanadateを使った実験で産生量が減少する。domainについても検討し、明らかにtype III ABC transportersに属するといっている。

close this sectionSequence

selected fasta
>putative ABC transporter ATP-binding/permease protein [PimB protein]
MVSTDSSSHEWRGPARRTLELLRPHRAPVTVAVALGIGGVVLNASGPMLLGRATDLIFDG
IRHTHGSRGIDFDAIARVLLTALALFAAASLLTLLQGRLVTAVVQRVVFALRRSVEAKLA
RLPLRYFDRHPAGEVLSRVTNDVDNLQQTLQQTLSQLITAAFSVLTMVVLMFALSPPLAL
IMLACVPVSAVIAARISRRAQPRFTQQWSATGALSAHVEEVYTGHALVKGFGRREQAERV
FDEHNDALYRAGARAQCVSGAIEPSMMFVSNLGYVVVAVVGALRVVSGALTIGDVQAFIL
YSRQFSQPIVEVAGIAARLQSALASAKRVHDLLDADEQGPDPERPARLPQARGHVRFDKV
GFRYSPDLPLIEDLCLTVEPGQTVAVVGPTGAGKTTLGNLLMRFYETDAGRILLDGTDIT
TLTREDLRSHIGLVLQDAWLFSGTIAENIAYGRPDATREEIVAAARATCADRFIRTLPDG
YDTVLDEESANVSAGEKQLITIARAFLARPSILLLDEATSSVDTRTEVLIQRAMHSLRAG
RTSFVIAHRLSTIRDADLILVMEDGRIVERGTHDQLLAAEGAYARLHATAGPDATAGPDA
TAGPDATAGPDATAAPRTSGISPSLS
selected fasta
>putative ABC transporter ATP-binding/permease protein [PimB protein]
ATGGTCTCGACGGACAGCTCTAGCCACGAGTGGCGCGGCCCGGCCCGCCGCACGCTGGAA
CTGCTGCGCCCGCACCGTGCCCCGGTGACCGTGGCGGTCGCCCTCGGCATCGGCGGCGTC
GTCCTCAACGCGTCGGGTCCGATGCTCCTCGGCAGGGCCACGGACCTCATCTTCGACGGC
ATCCGGCACACCCACGGATCCAGAGGCATCGACTTCGACGCGATCGCCCGTGTGCTGCTG
ACGGCGCTCGCGCTCTTCGCCGCCGCCTCCCTCCTCACCCTCCTCCAGGGGCGGCTGGTC
ACGGCCGTCGTCCAGCGTGTGGTCTTCGCACTGCGCCGTTCCGTGGAGGCCAAGCTCGCC
CGCCTGCCGCTGCGGTACTTCGACCGCCACCCGGCGGGGGAGGTGCTCAGCCGGGTGACC
AATGACGTCGACAACCTCCAGCAGACCCTCCAGCAGACCCTCAGCCAGCTGATCACGGCG
GCGTTCTCCGTGCTGACCATGGTGGTGCTGATGTTCGCCCTCTCGCCGCCGCTGGCCCTG
ATCATGCTGGCCTGCGTACCGGTGTCGGCCGTGATCGCGGCCCGGATCAGCCGGCGCGCA
CAGCCGCGGTTCACCCAGCAGTGGTCCGCGACCGGAGCGCTCAGCGCCCATGTCGAGGAG
GTCTACACCGGACATGCGCTGGTCAAGGGCTTCGGGCGGCGCGAGCAGGCCGAGCGGGTC
TTCGACGAGCACAACGACGCCCTGTACCGCGCCGGTGCGAGGGCCCAGTGCGTCTCCGGC
GCCATCGAACCGTCGATGATGTTCGTCTCCAACCTCGGCTATGTCGTCGTGGCGGTCGTC
GGCGCGCTGCGCGTGGTCTCCGGGGCCCTGACCATCGGCGACGTCCAGGCGTTCATCCTC
TACTCCCGGCAGTTCAGTCAGCCGATCGTCGAGGTCGCCGGCATCGCCGCCCGCCTCCAG
TCCGCCCTCGCCTCCGCGAAGCGGGTCCACGACCTCCTGGACGCCGACGAACAAGGCCCC
GACCCCGAGCGGCCCGCACGCCTCCCACAGGCCCGTGGACACGTCCGGTTCGACAAGGTC
GGTTTCCGCTACTCCCCCGACCTCCCCCTCATCGAGGACCTCTGCCTCACCGTCGAACCC
GGACAGACCGTGGCGGTCGTCGGTCCGACCGGCGCGGGCAAGACCACGCTGGGCAACCTC
CTCATGCGGTTCTACGAAACCGACGCGGGGCGCATCCTCCTGGACGGCACCGACATCACC
ACCCTGACCCGCGAGGACCTGCGCTCCCATATCGGCCTGGTGCTCCAGGACGCCTGGCTG
TTCTCCGGCACCATCGCCGAGAACATCGCCTACGGGCGCCCCGACGCGACGCGCGAGGAG
ATCGTGGCCGCCGCCCGCGCCACCTGCGCCGACCGCTTCATCCGCACCCTGCCGGACGGC
TACGACACCGTCCTGGACGAGGAGTCGGCCAACGTCAGCGCGGGCGAGAAGCAGCTCATC
ACCATTGCCCGGGCCTTCCTGGCCCGGCCCTCGATCCTGCTCCTGGACGAGGCGACCAGC
TCCGTCGACACCCGCACGGAAGTCCTCATCCAACGCGCGATGCACTCGCTGCGCGCGGGC
CGCACCAGCTTCGTCATAGCCCACCGGCTGTCCACCATCCGCGATGCGGACCTCATCCTC
GTCATGGAAGACGGCCGCATCGTCGAGCGCGGCACCCACGACCAGCTCCTGGCCGCCGAG
GGGGCCTACGCCCGGCTGCATGCGACCGCGGGGCCCGATGCGACGGCGGGGCCCGATGCG
ACGGCGGGGCCCGATGCGACGGCGGGGCCCGATGCGACGGCGGCGCCGCGTACCTCTGGC
ATTTCCCCCAGTCTTTCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001140 ABC transporter, transmembrane domain (Domain)
[31-308] 9.29999999999998e-51 PF00664
PF00664   ABC_membrane
IPR003439 ABC transporter-like (Domain)
[395-520] 9.50000000000001e-19 PF00005
PF00005   ABC_tran
[355-589] PS50893
PS50893   ABC_TRANSPORTER_2
IPR003593 AAA+ ATPase domain (Domain)
[380-572] 4.40000933643891e-18 SM00382
SM00382   AAA
IPR011527 ABC transporter, transmembrane domain, type 1 (Domain)
[11-345] 2.59998233910302e-80 SSF90123
SSF90123   ABC_TM_1
IPR017871 ABC transporter, conserved site (Conserved_site)
[492-506] PS00211
PS00211   ABC_TRANSPORTER_1
IPR017940 ABC transporter, integral membrane type 1 (Domain)
[31-321] PS50929
PS50929   ABC_TM1F
SignalP
[1-1] Signal-anchor
Eukaryota   
TMHMM
[31-53] Transmembrane (o-i) [74-96] Transmembrane (i-o) [172-194] Transmembrane (o-i) [271-293] Transmembrane (i-o)
Transmembrane 4   
Pimar_00100 Pimar_00100   Pimar_00120 Pimar_00120
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