Pimar_00140 : CDS information

Location

Organism	Streptomyces natalensis
Strain	ATCC 27448 (=NBRC 13367)
Entry name	Pimaricin
Contig	AJ278573
Start / Stop / Direction	59,533 / 60,729 / + [in whole cluster] 52,789 / 53,985 / + [in contig]
Location	59533..60729 [in whole cluster] 52789..53985 [in contig]
Type	CDS
Length	1,197 bp (398 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	PimG protein
Gene
Gene (GenBank)	pimG
EC number
Keyword	C-12 carboxy group formation
Note
Note (GenBank)
Reference	ACC Q9EW94 PmId [11094342] A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis. (Chem Biol. , 2000) comment pimaricin biosynthetic gene clusterの報告。 pimG: cytochrome P-450 monooxygenase pimDとpimGはP450-like monooxygenasesをコードしている模様。細菌のP450 proteinとのalignmentから、O2-binding siteの一部とheam-binding pocketの一部が高く保存されていることがわかる。 C4でのhydroxyl基の生成(あとでepoxideに変換される)と、pimaricinにおけるexocyclic carboxyl functionの構成におけるいくつかのステップは、cytochrome P450 monooxygenaseによって触媒されうる。
Related Reference	ACC Q6T2C1 NITE Rimoc_00060 PmId [16242652] A tailoring activity is responsible for generating polyene amide derivatives in Streptomyces diastaticus var. 108. (Chem Biol. , 2005) comment BLAST id83% Streptomyces diastaticus_rimG [Rimoc_00060]cytochrome P450(C-14 oxidation) RimG disruptant mutantでは、rimocidin and CE-108のC-14でCOOH基の代わりにCH3基を持つ化合物を産生。よってRimGは、このC-14での酸化を担う。 mutant産生noncarboxylated tetraenesは、cholesterol-containing membranesに対しての毒性が下がっている。
	ACC Q6W5Q7 NITE FR008_00090 PmId [19139241] Tailoring the P450 monooxygenase gene for FR-008/candicidin biosynthesis. (Appl Environ Microbiol. , 2009) comment BLAST id73% Streptomyces sp. FR-008_fscP [FR008_00090]cytochrome P450(C-18 oxidation) fscP deletion mutantがcarboxyl基を持たないFR-008 derivativesを産生することを確認。その産物は毒性が低い。また、fscP deletion mutantはfscP-fscFEを持ったplasmidで相補され、普通のFR-008/candicidin産生をwildレベルまで回復する。
	ACC Q9L4W8 NITE Nysta_00100 PmId [21764946] New nystatin-related antifungal polyene macrolides with altered polyol region generated via biosynthetic engineering of Streptomyces noursei. (Appl Environ Microbiol. , 2011) comment BLAST id69%, 1e-143 Streptomyces noursei_nysN [Nysta_00100]cytochrome P450(C-16 oxidation) 遺伝子操作による新規化合物の抗真菌活性、溶血作用を調査。 NysN不活化のあるmutantsではC-16がメチル基のまま。
	ACC F1CLB6 PmId [25952111] ( , ) comment BLAST id96% Streptomyces chattanoogensis_scnG ScnG [PMID: 25952111] 本文未確認。abstractのみ。 P450 monooxygenase gene scnGの不活化を通して以下の3つの新規pimaricin誘導体が作成された。 12-decarboxy-12-methyl pimaricin (1) 4,5-desepoxy-12-decarboxy-12-methyl pimaricin (2) 2-hydro-3-hydroxy-4,5-desepoxy-12-decarboxy-12-methyl pimaricin (3) P450 monooxygenase ScnDによって、(2)から(1)へと変換されることが遺伝子解析と生化学解析で証明された。scnG/scnD double mutationは(1)の産生を廃止し、(2)の収量を2.3倍に改善した。

Sequence

>putative cytochrome P450 [PimG protein]
MTYTDPAAPETDPPAVDFPQRKPGVPFPPPDYADYRDRKGLVLSQLSDGKRVWLVTRHED
VRAVLTSPSISSNPEHKGFPNVGNLGVPKQDQIPGWFVGMDSPEHDRFRKALIPEFTVRR
VRAMKPAIERTVDAQLDAMLAAGNTADLVADFALPIPSLVISALLGVPPADREFFESRTR
VLVSLRSSTDDDRMAAAKDLLRYINRLVEIKQKWGGDDLITRLLATGAIAPHEMSGVLML
LLIAGHETTANNIALGVVTLLANPQWIGDDRAVEETLRFHSVADLVSLRVAVQDVEIAGQ
LIKAGEGIVPLVAAANHDENAFECPHAFDPSRSARHHVAFGYGVHQCLGQNLVRIEMEVA
YRKLFERIPNLELAVPTDGLDIKYDGVLYGLNELPVRW

>putative cytochrome P450 [PimG protein]
ATGACGTACACAGACCCGGCCGCGCCCGAGACGGATCCGCCGGCCGTCGACTTTCCGCAG
CGCAAGCCCGGCGTGCCGTTCCCGCCGCCCGACTACGCCGACTACCGCGACCGGAAGGGG
CTCGTCCTCTCGCAGCTGTCCGACGGCAAACGGGTATGGCTGGTCACCCGGCACGAGGAC
GTACGCGCCGTACTGACCAGCCCGAGCATCAGCTCGAACCCCGAGCACAAGGGATTTCCC
AACGTCGGGAACCTGGGTGTGCCCAAGCAGGACCAGATCCCGGGCTGGTTCGTGGGCATG
GACTCCCCCGAGCACGACCGGTTCCGCAAGGCCCTCATCCCGGAGTTCACCGTCCGGCGG
GTACGCGCGATGAAGCCCGCGATCGAACGCACGGTGGACGCCCAACTGGACGCCATGCTG
GCCGCGGGCAACACCGCCGACCTCGTCGCCGACTTCGCCCTGCCCATCCCCTCCCTGGTG
ATCTCCGCACTGCTCGGCGTGCCGCCCGCCGACCGCGAGTTCTTCGAGTCCAGGACCCGC
GTCCTGGTCTCCCTCCGCTCCTCCACCGACGACGACCGGATGGCCGCCGCCAAGGACCTC
CTGCGGTACATCAACCGGCTCGTGGAGATCAAACAGAAGTGGGGCGGCGACGACCTCATC
ACCCGGCTGCTGGCCACCGGTGCCATCGCCCCCCACGAAATGTCCGGCGTGCTGATGCTC
CTGCTCATCGCCGGCCACGAGACCACGGCCAACAACATCGCCCTCGGCGTGGTCACCCTG
CTGGCGAACCCCCAATGGATCGGCGACGACCGGGCCGTGGAGGAGACCCTGCGCTTCCAC
TCCGTCGCCGACCTGGTGTCCCTGCGCGTCGCGGTCCAGGACGTGGAAATCGCCGGGCAG
CTCATCAAGGCGGGCGAGGGAATCGTGCCGCTGGTCGCCGCCGCCAATCATGACGAGAAC
GCCTTCGAATGCCCCCACGCCTTCGACCCGTCCCGGTCCGCCCGCCACCATGTGGCCTTC
GGCTACGGCGTACACCAATGCCTGGGACAGAACCTGGTGCGGATCGAGATGGAAGTCGCG
TACCGGAAACTCTTCGAGCGCATCCCGAACCTCGAACTCGCCGTGCCCACCGACGGGTTG
GACATCAAGTACGACGGCGTGCTCTACGGCCTGAACGAGCTGCCCGTCCGCTGGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 PR00385 P450 G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Pimar_00130 Pimar_00150