Pimar_00130 : CDS information

Location

Organism	Streptomyces natalensis
Strain	ATCC 27448 (=NBRC 13367)
Entry name	Pimaricin
Contig	AJ278573
Start / Stop / Direction	58,446 / 59,504 / + [in whole cluster] 51,702 / 52,760 / + [in contig]
Location	58446..59504 [in whole cluster] 51702..52760 [in contig]
Type	CDS
Length	1,059 bp (352 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative GDP-3-keto-6-deoxy-D-mannose C-3 aminotransferase
Product (GenBank)	PimC protein
Gene
Gene (GenBank)	pimC
EC number	2.6.1.-
Keyword	mycosamine
Note
Note (GenBank)
Reference	ACC Q9EW95 PmId [11094342] A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis. (Chem Biol. , 2000) comment pimaricin biosynthetic gene clusterの報告。 PimC: aminotransferase PimCはaminosugarsの合成に関与したaminotransferaseに似ている。
Related Reference	ACC Q93NX0 NITE Amph_00130 PmId [16079135] Biosynthesis of amphotericin derivatives lacking exocyclic carboxyl groups. (J Biol Chem. , 2005) comment 3rd(Q93NX0) 79%, 1e-166 Streptomyces nodosus_amphDII sugar aminotransferase amphDII geneがStreptomyces nodosusで削除されたとき、amphotericin aglyconeにC3でamino基を持たない(代わりにOH基を持つ)neutral deoxyhexoseの付着を招いた。
Related Reference	ACC Q6W5Q8 NITE FR008_00080 PmId [14652074] Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurally related polyene complex. (Chem Biol. , 2003) comment 5th(Q6W5Q8) 83%, 1e-165 Streptomyces sp. FR-008_fscMII GDP-3-keto-6-deoxy-mannose 3-aminotransferase fscMII破壊mutantではsugar moietyを持たないFR-008 aglyconesが主に産生された。そして低レベルではあるが、3-keto-6-deoxy-D-mannoseをsugar moietyとするものも得られた。 sugar moietyを持たないものが主なのはmycosamine glycosylTFの基質特異性が厳しいため。

Sequence

>putative GDP-3-keto-6-deoxy-D-mannose C-3 aminotransferase [PimC protein]
MSFKYPVSRPTLDGRELEYVTGAVGDGWISSQGPMVARFERAFADYNGVAHGVSCSSGTA
ALTLALRALGIGPGDEVIVPEFTMVASAWAVTYTGATPVFVDCGDDLNIDVTRIEEKITA
RTKAVMPVHIYGRRCDMDAVMDIAHQYNLRVVEDSAEAHGVRPVGDIACYSLFANKIITA
GEGGICLTDDPRLAGQLAHLRAMAFTKDHSFLHKKVAYNFRMTAMQAAVALAQVERLDDI
LATRREIERRYDEGLAGVPGITLMPPRDVLWMYDLRAERREELREFLAGESIETRLFFKP
MSRQPMYFHPDWPSLKAHTFAEDGLYLPTHTGLSATDQDFVIDRVRAFYGAS

>putative GDP-3-keto-6-deoxy-D-mannose C-3 aminotransferase [PimC protein]
ATGTCGTTCAAGTACCCCGTGTCCAGGCCCACTCTGGACGGCCGGGAGCTGGAGTATGTG
ACCGGTGCCGTCGGGGACGGCTGGATCTCGTCCCAGGGCCCGATGGTCGCGCGGTTCGAG
CGTGCCTTCGCGGACTACAACGGCGTCGCGCACGGCGTGTCCTGTTCGTCCGGGACGGCG
GCGCTCACGCTGGCGCTGCGGGCGCTGGGTATCGGCCCGGGGGACGAGGTGATCGTTCCG
GAGTTCACGATGGTCGCCTCGGCATGGGCGGTCACCTACACCGGTGCGACGCCCGTGTTC
GTGGACTGCGGAGACGACCTCAATATCGATGTCACGCGTATCGAGGAGAAGATCACGGCG
CGGACGAAGGCCGTGATGCCCGTCCACATCTATGGGCGGCGGTGCGATATGGACGCCGTG
ATGGATATCGCGCACCAGTACAACCTGCGGGTCGTGGAGGACAGTGCCGAGGCCCACGGT
GTCCGCCCGGTCGGGGACATCGCGTGCTACTCGCTGTTCGCCAACAAAATCATCACGGCG
GGCGAGGGCGGCATCTGCCTCACCGACGACCCCAGGCTGGCCGGGCAGCTGGCGCATCTG
CGCGCCATGGCGTTCACCAAGGACCACAGTTTCCTGCACAAGAAGGTCGCCTACAACTTC
CGGATGACGGCGATGCAGGCCGCCGTCGCGCTCGCCCAGGTCGAGCGGCTGGACGACATC
CTCGCCACGCGGCGGGAGATCGAGCGGCGCTATGACGAGGGGCTCGCCGGAGTTCCCGGT
ATCACGCTGATGCCGCCGCGGGACGTGCTGTGGATGTACGACCTGCGGGCCGAACGCCGC
GAGGAGCTACGGGAGTTCCTGGCCGGTGAGTCGATCGAGACCCGGCTGTTCTTCAAGCCG
ATGAGCCGGCAGCCCATGTACTTCCACCCCGACTGGCCCTCGCTCAAGGCGCATACGTTC
GCCGAGGACGGGCTGTATCTGCCCACCCACACCGGACTGTCCGCCACCGACCAGGACTTC
GTGATCGACCGGGTCCGCGCGTTCTACGGCGCGAGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family) PIRSF000390 PLP_StrS PF01041 DegT_DnrJ_EryC1 IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain) G3DSA:3.40.640.10 PyrdxlP-dep_Trfase_major_sub1 IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain) G3DSA:3.90.1150.10 PyrdxlP-dep_Trfase_major_sub2 IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain) SSF53383 PyrdxlP-dep_Trfase_major
SignalP	No significant hit
TMHMM	No significant hit

Pimar_00120 Pimar_00140