Pyolu_00010 Pyolu_00010   Pyolu_00030 Pyolu_00030

Pyolu_00020 : CDS information

close this sectionLocation

Organism
StrainPf-5
Entry namePyoluteorin
Contig
Start / Stop / Direction2,694 / 1,663 / - [in whole cluster]
2,694 / 1,663 / - [in contig]
Locationcomplement(1663..2694) [in whole cluster]
complement(1663..2694) [in contig]
TypeCDS
Length1,032 bp (343 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
ProductLysR family transcriptional activator
Product (GenBank)transcriptional activator of pyoluteorin biosynthetic genes
Gene
Gene (GenBank)pltR
EC number
Keyword
Note
Note (GenBank)
  • contains N-terminal helix-turn-helix binding motif; PltR
Reference
ACC
PmId
[10094695] Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5. (J Bacteriol. , 1999)
[27148187] The Rare Codon AGA Is Involved in Regulation of Pyoluteorin Biosynthesis in Pseudomonas protegens Pf-5. (Front Microbiol. , 2016)
comment
[PMID: 10094695]
pyoluteorin生合成clusterの5'側 pltM-pltGについての論文
pltR: transcriptional regulator @pathway-specific regulator

pyoluteorin biosynthesis genes pltB, pltE, and pltFの転写は、pltR mutantでより、wildでのほうが明らかに良い。従って、PltRはリンクしたpyoluteorin合成遺伝子のtranscriptional activatorである。
Related Reference
ACC
Q6TLK2
PmId
[17653674] LysR family factor PltR positively regulates Pyoluteorin production in a pathway-specific manner in Pseudomonas sp. M18. (Sci China C Life Sci. , 2007)
[18248796] PltR expression modulated by the global regulators GacA, RsmA, LasI and RhlI in Pseudomonas sp. M18. (Res Microbiol. , 2008)
[22761817] ( , )
comment
BLAST id62%
Pseudomonas sp. M18_pltR
PltR

Pseudomonas sp. M18もPyoluteorinを産生する。

---
[PMID: 17653674](2007) abstract

pltRの無発現mutant M18TRGはPyoluteorin産生が30%まで減少。
相補でwild-type M18におけるレベルに回復。
pltRの過剰発現は、wild-type M18の13倍のPlt産生を導いた。
PCA (Phenazine-1-carboxylic acid)産生は変化なし。
よってPltRがPlt産生においてpositive regulatorであることが示唆される。

また、mutant M18TRGにおけるPlt-lacZ transcriptional fusionの発現はwild-type M18と比べて明らかに減少し、PltRが転写レベルでPlt生合成遺伝子の発現を調節しうることがさらに証明された。

---
[PMID: 22761817](2012)
LysR-type transcriptional regulator PltRがpyoluteorin生合成オペロンpltLABCDEFGを活性化する機構について調査している。
PltR proteinは不可欠なbinding motif "lys box"としてpltLp領域へ直接的に結合し、pltLpが操縦するplt operon発現を転写活性する。

close this sectionSequence

selected fasta
>LysR family transcriptional activator [transcriptional activator of pyoluteorin biosynthetic genes]
MKALGVVNDIDLYISVTKTGSFSETGRLLGIPPSSVMRRINSLEKELETCLFNRSTKCLI
LTETGLLFLEHAKNISRCIGHARSEVKEHTASTLGVLKVSAPVAFGRRHVAPLLSRILNH
HPGLKIEFSLNDKALDPSIDNVDICIKLGILPDSNLIPTKLADMRRVLCASPEYIRQHGC
PQTLEDLYQHACLIHSTCSNFSLTWQFKVDGLLKKLMPSSRLSVNSSELLVDGALQGIGI
IHAPTWLVHEQIASGQLVSLLDEYCEADPQQGAIYALRARSSVVPAKTRLFINELKRSIG
STPYWDLPFEKEIPQTLATLHFDTAMHSALSRATTLQDKSQTS
selected fasta
>LysR family transcriptional activator [transcriptional activator of pyoluteorin biosynthetic genes]
ATGAAGGCGCTAGGCGTTGTCAATGACATAGACCTTTATATATCCGTAACTAAAACGGGT
AGTTTCTCCGAAACAGGAAGACTACTTGGCATCCCACCTTCTTCGGTGATGCGCAGAATC
AATAGCCTCGAAAAAGAACTTGAAACCTGTCTATTCAATCGATCGACAAAATGCTTGATA
CTTACCGAAACAGGCCTGCTCTTTCTCGAACATGCAAAGAACATCTCGAGATGCATTGGG
CATGCTAGATCAGAAGTAAAAGAACACACTGCATCGACCCTTGGCGTTCTCAAGGTTTCA
GCACCCGTGGCCTTTGGACGGCGCCATGTAGCGCCTTTATTGAGCAGAATACTGAACCAT
CATCCCGGTTTGAAAATTGAATTCTCGCTTAACGACAAAGCTCTCGACCCCAGCATCGAT
AACGTCGATATCTGTATCAAGCTGGGCATATTGCCGGACAGTAATCTGATTCCAACGAAA
CTTGCGGATATGCGGCGCGTGCTCTGCGCAAGCCCGGAATACATCCGGCAACACGGCTGT
CCGCAAACTCTTGAAGACCTGTACCAACATGCCTGCCTGATCCACAGCACCTGCAGCAAC
TTCTCACTGACCTGGCAATTCAAGGTCGATGGCCTGCTCAAGAAGCTCATGCCCAGCAGC
CGGCTATCGGTCAACAGTTCCGAGTTGCTGGTGGACGGCGCCCTTCAGGGCATAGGAATC
ATCCACGCGCCCACCTGGCTGGTGCATGAACAGATCGCCAGCGGCCAACTGGTGTCGCTG
CTCGACGAATATTGCGAGGCCGATCCGCAACAGGGAGCGATCTACGCACTGAGGGCACGC
AGCAGCGTTGTCCCGGCCAAGACCCGCCTGTTCATCAATGAGTTGAAACGCTCGATCGGC
AGCACGCCTTACTGGGACTTGCCGTTTGAAAAGGAAATACCGCAGACCCTGGCCACCCTG
CATTTCGATACCGCAATGCATTCAGCGCTTTCCAGAGCGACCACCTTGCAAGACAAGAGC
CAGACATCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000847 Transcription regulator HTH, LysR (Domain)
[8-65] 2.3e-12 PF00126
PF00126   HTH_1
[1-62] 12.773 PS50931
PS50931   HTH_LYSR
IPR005119 LysR, substrate-binding (Domain)
[95-298] 6.5e-31 PF03466
PF03466   LysR_substrate
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[9-89] 7.1e-15 G3DSA:1.10.10.10
G3DSA:1.10.10.10   no description
SignalP No significant hit
TMHMM No significant hit
Pyolu_00010 Pyolu_00010   Pyolu_00030 Pyolu_00030
Page top