Pyolu_00070 : CDS information

Location

Organism	Pseudomonas fluorescens
Strain	Pf-5
Entry name	Pyoluteorin
Contig	AF081920
Start / Stop / Direction	17,642 / 19,276 / + [in whole cluster] 17,642 / 19,276 / + [in contig]
Location	17642..19276 [in whole cluster] 17642..19276 [in contig]
Type	CDS
Length	1,635 bp (544 aa)

Annotation

Category	5.3 uncharacterized protein (biosynthesis involved)
Product	hypothetical protein
Product (GenBank)	PltD
Gene
Gene (GenBank)	pltD
EC number
Keyword
Note
Note (GenBank)	similar to halogenases but no obvious NAD(P)H binding region
Reference	ACC Q9X3R2 PmId [10094695] Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5. (J Bacteriol. , 1999) comment pyoluteorin生合成clusterの5'側 pltM-pltGについての論文 pltD: halogenase @pyrrole chlorination PltA, PltD, and PltMは、二次代謝産物をchlorinateするhalogenase enzymesの新しいクラスに属する。 NADH cofactor bindingに必要な構造を担うmotifをPltAとPltMは持っているが、PltDは持っていないので触媒機能もないと考えられる。しかし、PltDを破壊するとpyoluteorinは産生されないので、pyoluteorin合成には必要なgeneである。

Sequence

>hypothetical protein [PltD]
MNDVQSGKAPEHYDILLAGNSISVIMLAACLARNKVRVGLLRNRQMPPDLTGEATIPYTS
MIFELIADRYGVPEIKNIARTRDIQQKVMPSSGVKKNLGFIYHQRSRAVDLGQALQFNVP
SEHGENHLFRPDIDAYLLAAAIGYGAQLVEIDNSPEVLVEDSGVKVATALGRWVTADFMV
DGSQGGQVLARQAGLVSQASTQKTRTLEFSTHMLGVVPFDECVQGDFPGQWHGGTLHHVF
DGGWVGVIPFNNHQHSRNPLVSVLVSLREDLCPSMDGDQVLAGLIELYPGLGRHLSGARR
VREWVLRQPPRQVYRTALERRCLMFDEGAASNDLLFSRKLSNAAELVLALAHRLIKAAHS
GDYRSPALNDFVLTQDSIISLSDRIALAAYVSFRDPELWNAFARVWLLQSIAATITARKI
NDAFAKDLDPRVFDEIDQLAEDGFWMPLYRGYKDILNTTLGLCDDVKSAKVSAAHAASSI
FAELANASFVPPIFDFANPHARVYQLTTLRKLKALWWGLMQVPSEVGRLIFYRSFRKPSL
RKES

>hypothetical protein [PltD]
ATGAACGATGTGCAGTCTGGCAAGGCGCCAGAGCATTACGACATTCTCTTGGCGGGCAAC
AGCATCAGCGTGATCATGCTCGCCGCCTGCCTGGCCCGGAACAAGGTCCGGGTCGGTTTG
TTGCGCAACCGGCAGATGCCCCCCGACCTTACCGGTGAGGCGACGATTCCCTATACCTCG
ATGATTTTCGAGCTGATTGCCGACCGCTATGGCGTGCCGGAAATAAAGAATATCGCCCGC
ACCCGGGATATCCAGCAGAAGGTGATGCCGTCTTCCGGGGTCAAGAAGAACCTCGGGTTC
ATCTATCACCAGCGCAGCCGGGCGGTGGACCTGGGCCAGGCGCTGCAATTCAACGTGCCC
TCCGAGCATGGCGAGAACCATCTGTTCAGGCCCGATATCGATGCCTATCTGCTGGCGGCG
GCCATCGGTTATGGCGCGCAGCTGGTGGAGATCGATAACAGCCCAGAGGTGCTGGTCGAG
GACAGCGGGGTCAAGGTAGCTACGGCACTGGGGCGCTGGGTCACTGCCGATTTCATGGTT
GATGGCAGCCAGGGCGGCCAGGTGCTGGCGCGGCAGGCTGGCCTGGTCAGCCAGGCTTCG
ACGCAGAAGACCCGGACCCTGGAATTCTCCACTCATATGCTCGGGGTGGTGCCGTTCGAT
GAGTGCGTGCAGGGCGATTTTCCCGGCCAGTGGCATGGCGGCACTCTGCATCACGTGTTC
GATGGGGGCTGGGTGGGGGTCATCCCGTTCAACAACCATCAGCACTCGCGCAACCCTTTG
GTCAGCGTGCTGGTTTCACTGCGTGAGGACCTCTGCCCGAGCATGGACGGCGACCAGGTC
CTGGCCGGCCTGATCGAGCTGTACCCCGGCCTGGGGCGGCACCTGTCCGGCGCCCGGCGG
GTGCGCGAGTGGGTGCTGCGCCAGCCGCCCCGGCAGGTCTATCGCACGGCGCTCGAACGC
CGCTGCCTGATGTTCGACGAGGGCGCCGCGAGCAACGATCTGTTGTTCTCGCGCAAGCTG
TCCAATGCTGCGGAACTGGTTCTGGCCCTGGCGCACCGGCTGATCAAGGCGGCGCACAGC
GGTGACTACCGCAGCCCGGCCCTGAATGATTTTGTCCTGACCCAGGACAGCATCATCAGC
TTGAGTGACCGGATCGCCTTAGCGGCTTATGTGTCGTTTCGCGACCCCGAGTTGTGGAAT
GCCTTCGCCCGTGTCTGGCTGCTGCAGTCGATTGCCGCCACCATCACCGCGCGCAAGATC
AACGATGCCTTTGCCAAGGACCTGGACCCGCGAGTGTTCGATGAAATCGACCAGCTCGCA
GAGGACGGTTTCTGGATGCCTCTGTATCGGGGGTACAAGGATATTCTCAACACTACGCTG
GGCCTTTGTGATGACGTCAAAAGCGCCAAGGTCTCTGCTGCGCACGCGGCGAGCAGCATC
TTTGCGGAGCTTGCCAACGCCAGTTTTGTTCCGCCTATTTTTGATTTTGCTAATCCTCAC
GCTCGTGTCTATCAACTGACCACCTTGAGAAAGCTCAAGGCGCTCTGGTGGGGCCTGATG
CAAGTGCCCTCAGAGGTCGGACGGCTGATTTTCTATCGATCCTTCAGAAAACCTTCCCTG
CGCAAGGAGAGTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Pyolu_00060 Pyolu_00080