Pyolu_00080 : CDS information

Location

Organism	Pseudomonas fluorescens
Strain	Pf-5
Entry name	Pyoluteorin
Contig	AF081920
Start / Stop / Direction	19,278 / 20,420 / + [in whole cluster] 19,278 / 20,420 / + [in contig]
Location	19278..20420 [in whole cluster] 19278..20420 [in contig]
Type	CDS
Length	1,143 bp (380 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	L-prolyl-PCP dehydrogenase
Product (GenBank)	putative acyl-CoA dehydrogenase
Gene
Gene (GenBank)	pltE
EC number	1.3.99.-
Keyword	pyrrole ring moiety
Note
Note (GenBank)	PltE
Reference	ACC Q9X3R3 PmId [10094695] Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5. (J Bacteriol. , 1999) [11880032] Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis. (Chem Biol. , 2002) comment [PMID: 10094695](1999) pyoluteorin生合成clusterの5'側 pltM-pltGについての論文 pltE: acyl-CoA dehydrogenase @pyrrole synthesis PltEは多くのflavin-dependent acyl coenzyme A(acyl-CoA) dehydrogenasesに似ている。触媒に重要な残基も保存されており、pyoluteorin pathway内のdehydrogenationを触媒する可能性が高い。 --- [PMID: 11880032](2002) pltEをE.coliで過剰発現したPltEの精製はうまくいかなかった。そこでPltEの反応産物を加水分解処理してPCPから外し、HPLCで分離したところ、標準的なpyrrolyl-2-carboxylateに一致するピークを検出。1H-NMRでもこれが標準的なpyrrolyl-2-carboxylateに一致することを確認。HPLCで見られるもうひとつのピークは、加水分解処理による副産物と思われる。同時に検討されているRedWは精製が可能であり、・反応にL-proline、ORF11(PltFに相当)、holo-ORF9(PltLに相当)、ATPが必要なこと、・PltEと同じ産物を得ることが確かめられている。

Sequence

>L-prolyl-PCP dehydrogenase [putative acyl-CoA dehydrogenase]
MDFNYDDTQKKHAAMIAQVCAEQLAACGNEHSRYFTARQWAICGEAGLLGLSIPREYGGQ
GLGALSTAIAMHAFGLGCTDMGLVFAAAAHQFACAMPIVEFATAETKRDVLPKLASGEFI
GSNAITEPEAGSDSSNLKSRAWPQADGSYRLDGHKSFAGNAPIADIFVTYATTQPEYGAL
GVSGFIVHRSSAGLRVSEPLDKVCLRSCPAGEVFFDDCRVPEVNRLGEEGQGRQVFQSSM
GWERACLFAAFLGMMERQLEQTIEHARTRRQFGKPIGDNQAVSHRIAQMKLRLESARLLL
FRACWGMDQGDPGQLNIALSKLAISEGALASSIDAVRIFGGRGCLESFGIEAMLRDSIGT
TIFSGTSDMQHEIIARELKL

>L-prolyl-PCP dehydrogenase [putative acyl-CoA dehydrogenase]
ATGGACTTCAACTACGACGATACCCAGAAAAAACATGCGGCCATGATCGCCCAGGTGTGT
GCCGAGCAATTGGCGGCCTGTGGCAATGAACACTCGCGGTATTTCACTGCCCGGCAATGG
GCGATCTGCGGCGAGGCCGGATTGCTGGGGCTGTCGATTCCCCGGGAATACGGTGGCCAG
GGCCTGGGTGCACTGTCGACGGCCATTGCCATGCACGCCTTTGGCCTGGGTTGCACAGAC
ATGGGCCTGGTGTTCGCGGCGGCGGCCCACCAGTTTGCCTGTGCGATGCCGATCGTCGAG
TTCGCAACAGCGGAAACCAAACGCGATGTGTTGCCCAAACTCGCCAGCGGTGAATTCATC
GGCTCCAACGCGATCACCGAACCCGAGGCCGGCTCCGACTCCAGCAATTTGAAGAGCCGT
GCCTGGCCCCAGGCCGATGGCAGTTATCGCCTTGACGGCCACAAGAGCTTTGCCGGCAAT
GCGCCGATTGCCGACATCTTCGTGACCTATGCCACCACCCAGCCCGAGTACGGTGCCCTG
GGGGTCAGCGGTTTTATCGTCCACCGCAGCAGTGCGGGGCTCAGGGTCAGTGAGCCCCTG
GACAAGGTATGCCTGAGAAGCTGCCCCGCGGGTGAAGTGTTCTTTGACGATTGCAGGGTT
CCTGAGGTCAACCGCCTGGGTGAGGAGGGGCAGGGCCGGCAGGTGTTCCAGAGCTCCATG
GGCTGGGAGCGTGCCTGCCTGTTCGCAGCGTTCCTGGGGATGATGGAGCGGCAACTGGAA
CAGACCATCGAGCATGCGCGCACCCGGCGCCAGTTTGGCAAGCCGATTGGCGACAACCAG
GCGGTTTCGCACCGTATCGCGCAAATGAAGCTGCGCCTGGAGTCGGCGCGGTTGCTGCTG
TTCCGGGCGTGCTGGGGCATGGACCAGGGCGATCCGGGGCAGCTCAACATTGCCCTGTCG
AAACTGGCCATCAGTGAAGGGGCGCTGGCATCTAGCATCGATGCGGTGAGGATTTTCGGC
GGCCGGGGCTGCCTGGAGTCTTTCGGGATCGAGGCGATGCTGCGCGACTCCATCGGCACT
ACGATCTTTTCCGGCACCAGCGACATGCAGCACGAGATCATTGCCCGGGAGCTGAAGCTA
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006090 Acyl-CoA oxidase/dehydrogenase, type 1 (Domain) PF00441 Acyl-CoA_dh_1 IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain (Domain) PF02770 Acyl-CoA_dh_M G3DSA:2.40.110.10 no description IPR006092 Acyl-CoA dehydrogenase, N-terminal (Domain) PF02771 Acyl-CoA_dh_N IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal (Domain) G3DSA:1.20.140.10 no description SSF47203 Acyl-CoA dehydrogenase C-terminal domain-like IPR009100 Acyl-CoA dehydrogenase/oxidase (Domain) SSF56645 Acyl-CoA dehydrogenase NM domain-like IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal (Domain) G3DSA:1.10.540.10 no description
SignalP	No significant hit
TMHMM	No significant hit

Pyolu_00070 Pyolu_00090