Pyolu_00150 Pyolu_00150   Pyolu_00170 Pyolu_00170

Pyolu_00160 : CDS information

close this sectionLocation

Organism
StrainPf-5
Entry namePyoluteorin
Contig
Start / Stop / Direction28,518 / 30,014 / + [in whole cluster]
28,518 / 30,014 / + [in contig]
Location28518..30014 [in whole cluster]
28518..30014 [in contig]
TypeCDS
Length1,497 bp (498 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative outer membrane protein
Product (GenBank)PltO
Gene
Gene (GenBank)pltO
EC number
Keyword
  • type I secretion system
Note
Note (GenBank)
  • predicted TolC-type outer membrane efflux protein
Reference
ACC
PmId
[16269724] Reciprocal regulation of pyoluteorin production with membrane transporter gene expression in Pseudomonas fluorescens Pf-5. (Appl Environ Microbiol. , 2005)
comment
pyoluteorin生合成clusterの3'側 pltZ-orf1についての論文
pltO: outer membrane protein or channel

TolC-type outer membrane efflux proteinのためのコンセンサスモデルに、PltOのAA配列はマッチする。
PltOを構成する2つのrepeatはどちらもこのprotein familyのコンセンサス配列にマッチする。
N末にtransmembrane domainが予測され、膜局在が考えられる。
Related Reference
ACC
Q4VWC6
PmId
[16581203] Identification and characterization of a putative ABC transporter PltHIJKN required for pyoluteorin production in Pseudomonas sp. M18. (Gene. , 2006)
comment
4th(Q4VWC6) 58%, 1e-157
Pseudomonas sp. M18_pltN
Outer membrane channel protein

Pseudomonas sp. M18もPyoluteorinを産生する。
gene nameはP. fluorescens Pf-5と一致していない。

---
配列から、pltHIJKN gene productsが典型的な3つの要素のABC export systemに組み込まれることが示唆される。
そのシステムは、
・inner membrane ABC transporter PltIJK
・membrane fusion protein PltH
・outer membrane efflux protein PltN
からなる。

pltH or pltI 欠陥mutantではPlt非産生。
pltHIJKN gene clusterの過剰発現でPlt産生増加。
pltHIJKN gene clusterの異種性発現で外因性Pltへの抵抗性あり。
外因性Pltは、Plt生合成gene clusterとABC transporter gene cluster pltHIJKN の両方の発現を転写レベルで誘導する。

close this sectionSequence

selected fasta
>putative outer membrane protein [PltO]
MKTTHSGYLRNGLTLAMLLAITACTVGPDFKAPAVTSPERWTDWHSGPPASSVPGKAPAT
TSQTADGNWWQVFGDPVLDQLQAQVREGSPDLHTALLRFAQVRLQRQIVASLETPEVSFS
AAASRNRQSRYAPNNRMLEALGSDSDALEKVLTDPYSLYQAGFDVSWELDLWGRVSRLGE
AAQAEVDGAAATLDDVLLSVSSELARNYFEVRTAQRQTRLLEQEAQILAAHLQVVAVQAR
EGEEDGFAVERQEARLASLHAQIPGWKALHTQAGNRIALLLGEHPGALETLLAERPDNAL
SRPLPNLQLGLPGDLARERPDIRAAQARLHRATAGIGVAEAELYPSVMLGADFGFESYKS
GQFADWSSRTWSVGPRLDLPLFDRGRRSKTVVLRTLEQQEAAVAFHRTVLAAWQEVDDAM
SRYHNEYQRAAHLKDSYDSKARTYEWTRVRYAAGEASYLEELEAQRTVLEAQRDLVNSDS
QLRTHLISIYKSMGGHST
selected fasta
>putative outer membrane protein [PltO]
ATGAAAACTACTCACTCTGGATACCTGCGTAATGGGCTGACGCTGGCCATGCTGCTGGCC
ATCACCGCCTGCACCGTCGGGCCCGATTTCAAGGCGCCGGCAGTTACCAGCCCGGAACGC
TGGACGGATTGGCACAGTGGCCCGCCAGCGTCTTCGGTGCCGGGCAAAGCCCCGGCGACC
ACCTCGCAAACCGCAGACGGCAACTGGTGGCAGGTGTTTGGCGACCCGGTGCTGGACCAG
CTGCAGGCCCAGGTGCGCGAAGGCAGCCCGGACCTGCACACGGCCTTGCTGCGGTTTGCC
CAGGTGCGGTTGCAACGCCAGATAGTCGCTTCCCTGGAAACGCCCGAAGTGTCCTTTTCC
GCGGCGGCTTCGCGAAACCGGCAAAGCCGGTATGCGCCGAACAATCGAATGCTTGAAGCC
TTGGGCAGCGACAGTGATGCCCTGGAAAAGGTGCTTACCGACCCTTATAGCCTGTACCAG
GCCGGGTTCGACGTTTCATGGGAGCTGGATCTCTGGGGGCGGGTGTCACGCCTGGGTGAA
GCCGCCCAGGCCGAGGTCGATGGTGCGGCCGCGACCCTGGACGACGTGTTGCTCAGTGTC
TCCAGCGAGCTGGCGCGAAACTACTTCGAAGTGCGTACCGCACAGCGCCAGACCCGTCTT
CTGGAGCAGGAGGCACAGATCCTGGCCGCGCACCTGCAAGTGGTTGCCGTCCAGGCCCGG
GAGGGCGAGGAAGACGGGTTCGCGGTGGAACGCCAAGAGGCACGGCTGGCCTCGCTGCAT
GCACAGATACCGGGTTGGAAGGCGCTGCACACCCAGGCGGGCAACCGTATTGCGCTGTTG
CTGGGGGAGCATCCCGGAGCCCTGGAAACCTTGCTCGCGGAGCGGCCTGATAATGCCTTG
AGCCGCCCGCTGCCCAACCTGCAGTTGGGCCTGCCTGGCGACCTGGCCCGGGAGCGTCCG
GACATCCGCGCGGCCCAGGCCCGCTTGCATCGGGCGACGGCGGGCATCGGAGTGGCCGAG
GCCGAACTGTACCCGAGCGTGATGCTGGGGGCCGACTTCGGCTTCGAATCCTACAAGAGC
GGCCAGTTTGCCGACTGGAGCAGCCGCACCTGGTCGGTAGGGCCGCGCCTGGACCTGCCG
CTGTTCGACCGTGGGCGGCGCAGCAAGACCGTGGTCCTGCGCACACTCGAACAGCAAGAG
GCGGCCGTGGCATTTCACCGGACCGTACTGGCAGCCTGGCAGGAAGTGGACGATGCGATG
AGCCGCTACCACAACGAATACCAGCGTGCCGCGCACCTCAAGGACAGCTATGACAGCAAG
GCCCGCACCTATGAGTGGACCCGCGTCCGGTACGCGGCCGGGGAGGCCAGCTACCTGGAG
GAACTGGAAGCGCAGCGCACCGTGCTGGAGGCGCAGCGCGACCTGGTCAATAGCGACAGT
CAACTGCGTACTCACCTGATCAGCATTTACAAATCCATGGGTGGGCATTCCACCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003423 Outer membrane efflux protein (Family)
[78-264] 1.4e-17 PF02321 [316-494] 1.9e-19 PF02321
PF02321   OEP
IPR010131 RND efflux system, outer membrane lipoprotein, NodT (Family)
[16-495] 6.9e-89 TIGR01845
TIGR01845   outer_NodT: efflux transporter, outer membrane fac
SignalP
[1-24] 0.841 Signal
Eukaryota   
[1-34] 0.997 Signal
Bacteria, Gram-positive   
[1-31] 0.875 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Pyolu_00150 Pyolu_00150   Pyolu_00170 Pyolu_00170
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