Pyolu_00140 Pyolu_00140   Pyolu_00160 Pyolu_00160

Pyolu_00150 : CDS information

close this sectionLocation

Organism
StrainPf-5
Entry namePyoluteorin
Contig
Start / Stop / Direction27,388 / 28,506 / + [in whole cluster]
27,388 / 28,506 / + [in contig]
Location27388..28506 [in whole cluster]
27388..28506 [in contig]
TypeCDS
Length1,119 bp (372 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.3 transport
Productputative ABC transporter permease protein
Product (GenBank)PltN
Gene
Gene (GenBank)pltN
EC number
Keyword
  • type I secretion system
Note
Note (GenBank)
  • predicted transmembrane subunit of ABC transporter
Reference
ACC
PmId
[16269724] Reciprocal regulation of pyoluteorin production with membrane transporter gene expression in Pseudomonas fluorescens Pf-5. (Appl Environ Microbiol. , 2005)
comment
pyoluteorin生合成clusterの3'側 pltZ-orf1についての論文
pltN: transmembrane subunit of ABC transporter

pltK and pltNは、ABC transporterのtransmembrane domain subunitsのコンセンサス配列に似ている。
Related Reference
ACC
Q4VWC7
PmId
[16581203] Identification and characterization of a putative ABC transporter PltHIJKN required for pyoluteorin production in Pseudomonas sp. M18. (Gene. , 2006)
comment
3rd(Q4VWC7) 80%, 1e-164
Pseudomonas sp. M18_pltK
Inner membrane permease protein

Pseudomonas sp. M18もPyoluteorinを産生する。
gene nameはP. fluorescens Pf-5と一致していない。

---
配列から、pltHIJKN gene productsが典型的な3つの要素のABC export systemに組み込まれることが示唆される。
そのシステムは、
・inner membrane ABC transporter PltIJK
・membrane fusion protein PltH
・outer membrane efflux protein PltN
からなる。

pltH or pltI 欠陥mutantではPlt非産生。
pltHIJKN gene clusterの過剰発現でPlt産生増加。
pltHIJKN gene clusterの異種性発現で外因性Pltへの抵抗性あり。
外因性Pltは、Plt生合成gene clusterとABC transporter gene cluster pltHIJKN の両方の発現を転写レベルで誘導する。

close this sectionSequence

selected fasta
>putative ABC transporter permease protein [PltN]
MDKLMNFLRELLFLFQKEFLAIVKDPANRVILIAPAIVQSLLFGYGATYDLNYVPYAVLD
QSQGRASTELLARFDASGVFARVSTLTSVDEIAPVIDRGSALLVLHIAADFDERLSRHEN
AGLQLILDGRNSTTAGMAAGHIANLVADFNQQFLAVDNAPVRLETRAWFNPNLQTRWNIV
PGLIAALSMIQTLMLAALSVAREREQGTFDQLLVTPLTPFVILLGKALPSVLIGLLQSSL
ILLIGLFWFKIPMIGSVLDLYLGLFVFTSACVGIGLSISALSANMQQAMVYTFVLMMPLI
LLSGLITPVHSMPEALQLLTYLDPLRFAIDLVRRIYLEGATLADVSWNLVPMLAVALVTL
PLAAWLFRNRLV
selected fasta
>putative ABC transporter permease protein [PltN]
ATGGACAAGCTGATGAATTTCCTACGCGAGTTGCTGTTCCTGTTTCAGAAGGAGTTCCTG
GCCATCGTCAAGGACCCGGCCAACCGGGTGATCCTGATTGCCCCGGCGATCGTCCAGTCA
TTGCTGTTCGGCTACGGCGCCACCTACGACCTGAACTACGTGCCCTATGCCGTGCTGGAT
CAGAGCCAGGGGCGCGCTTCGACAGAGCTTTTGGCACGCTTCGATGCCTCCGGGGTATTT
GCCCGAGTGTCGACCCTGACCAGCGTCGATGAGATCGCCCCGGTGATCGACCGGGGCAGT
GCCTTGCTGGTGCTGCATATCGCCGCAGACTTCGACGAGCGCCTGAGCCGGCATGAAAAC
GCCGGCTTGCAGTTGATCCTGGATGGGCGTAACTCGACGACCGCGGGAATGGCGGCCGGG
CATATCGCCAACCTGGTGGCTGACTTCAACCAGCAGTTCCTGGCGGTGGACAACGCGCCG
GTACGCCTGGAGACCCGTGCCTGGTTCAACCCCAACCTGCAAACCCGCTGGAACATAGTG
CCGGGCCTGATCGCCGCGCTGAGCATGATCCAGACCCTGATGCTGGCGGCGTTGTCGGTA
GCCAGGGAGCGCGAGCAGGGCACCTTCGACCAACTGCTGGTGACTCCGCTGACGCCTTTC
GTGATCCTGCTGGGCAAGGCCCTGCCGTCGGTACTGATCGGTCTCTTGCAGTCCAGCCTG
ATCCTCCTCATCGGCCTGTTCTGGTTCAAGATCCCCATGATCGGCTCCGTGCTGGACCTG
TACCTGGGGCTGTTCGTCTTCACCAGCGCCTGCGTGGGCATCGGGCTGTCGATCTCGGCG
CTGTCGGCCAACATGCAGCAGGCCATGGTGTACACCTTCGTGCTGATGATGCCGCTGATC
CTGCTCTCCGGCCTGATCACCCCGGTGCACAGCATGCCCGAGGCCCTGCAACTGCTGACC
TATCTCGATCCGCTGCGCTTTGCCATCGACCTGGTCAGGAGGATCTACCTGGAGGGGGCA
ACGCTGGCGGATGTGTCCTGGAACCTGGTGCCCATGCTTGCCGTTGCGCTGGTGACCCTG
CCGCTGGCTGCGTGGCTGTTTCGCAATCGATTGGTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000412 ABC-2 (Family)
[139-370] 40.058 PS51012
PS51012   ABC_TM2
IPR013526 ABC-2 transporter (Family)
[257-281] 4.8e-08 PR00164 [294-313] 4.8e-08 PR00164 [314-333] 4.8e-08 PR00164
PR00164   ABC2TRNSPORT
SignalP No significant hit
TMHMM
[179-201] Transmembrane (o-i) [227-249] Transmembrane (i-o) [259-281] Transmembrane (o-i) [288-310] Transmembrane (i-o) [345-367] Transmembrane (o-i)
Transmembrane 5   
Pyolu_00140 Pyolu_00140   Pyolu_00160 Pyolu_00160
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