Rapam_00060 Rapam_00060   Rapam_00080 Rapam_00080

Rapam_00070 : CDS information

close this sectionLocation

Organism
StrainNRRL 5491
Entry nameRapamycin
Contig
Start / Stop / Direction6,703 / 7,365 / + [in whole cluster]
6,703 / 7,365 / + [in contig]
Location6703..7365 [in whole cluster]
6703..7365 [in contig]
TypeCDS
Length663 bp (220 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative two-component system response regulator
Product (GenBank)response regulator
GeneorfR
rapR
Gene (GenBank)orfR
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995)
[8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996)
comment
[PMID: 7644502](1995)
rapamycin生合成gene clusterの報告。配列解析。
ORF R: response regulator
詳細記載なし

---
[PMID: 8635730](1996)
PKS flanking genesの配列解析。
orfR and orfSは翻訳共役しそう。
その遺伝子産物は細菌のtwo-component response sensor and regulator systemを構成する。
Asp51がphosphorylationのsiteだと予測される。
Related Reference
ACC
P0A4I2
PmId
[8885276] The cutRS signal transduction system of Streptomyces lividans represses the biosynthesis of the polyketide antibiotic actinorhodin. (Mol Microbiol. , 1996)
comment
BLAST id53%
Streptomyces lividans_cutR
Transcriptional regulatory protein CutR

CutRSは移行期と静止期に転写されている。
CutR, CutSの変異体では二次代謝産物であるActinorhodinの合成が促進される。
この表現型はCutRを戻すと回復する。
S. coelicolorではCutRSはActinorhodinの合成を抑制する働きがある。

close this sectionSequence

selected fasta
>putative two-component system response regulator [response regulator]
MRVLVAEDHRVLARTIATGLRRQAMAVDVAAAADEAERMCLLTAYDVLILDRDLPVLSGD
EVCRRLRELQDPPRILMLTAAGELTDKIYGLTTLGADDYLAKPFDFSELVARVRTLSRRA
PKQPPTVLRFASITLDETRREVSVDGRPLELTPKELAVLHLLLVAGGAPVSHDELVRTVW
DEHLDPRTSAVRATVSRLRGKLGDPGLILADKGEGYRLCD
selected fasta
>putative two-component system response regulator [response regulator]
ATGAGAGTTCTGGTAGCCGAGGACCACCGTGTTCTCGCCCGCACCATCGCCACCGGCCTG
CGCCGCCAGGCGATGGCCGTCGACGTGGCGGCCGCCGCCGACGAGGCCGAGCGGATGTGC
CTGCTCACCGCCTACGACGTGCTGATCCTCGACCGGGATCTGCCGGTCCTGAGCGGCGAC
GAGGTGTGCCGACGGCTGCGCGAACTCCAGGACCCTCCACGGATCCTGATGCTGACCGCG
GCCGGCGAGCTGACGGACAAGATCTACGGCCTCACGACACTCGGCGCCGACGACTACCTG
GCGAAACCCTTCGATTTCAGCGAACTGGTCGCCCGGGTACGGACGTTGAGCCGTCGCGCA
CCGAAGCAGCCCCCTACCGTGCTGCGCTTCGCGAGCATCACCCTGGACGAGACGCGGCGC
GAGGTGTCCGTGGACGGCCGCCCGCTGGAGCTGACCCCGAAGGAGCTCGCGGTCCTGCAC
CTGCTGCTTGTGGCCGGGGGCGCGCCGGTGTCGCACGACGAACTCGTCCGCACCGTCTGG
GACGAGCACCTCGACCCGCGCACCAGCGCGGTCCGCGCCACCGTCAGCCGGCTGCGCGGC
AAACTCGGCGACCCCGGCCTCATTCTCGCCGACAAGGGGGAGGGTTACCGGCTGTGCGAC
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001789 Signal transduction response regulator, receiver domain (Domain)
[3-114] 1.1e-20 PF00072
PF00072   Response_reg
[2-117] PS50110
PS50110   RESPONSE_REGULATORY
[1-113] 3.29999077503323e-33 SM00448
SM00448   REC
IPR001867 Signal transduction response regulator, C-terminal (Domain)
[146-218] 8.20000000000001e-16 PF00486
PF00486   Trans_reg_C
[146-218] 1.70000295590054e-20 SM00862
SM00862   Trans_reg_C
IPR011006 CheY-like superfamily (Domain)
[1-188] 7.30000590915208e-31 SSF52172
SSF52172   CheY_like
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[123-218] 1e-23 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
SignalP
[1-26] 0.231 Signal
Eukaryota   
[1-26] 0.151 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Rapam_00060 Rapam_00060   Rapam_00080 Rapam_00080
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