Rapam_00230 : CDS information

Location

Organism	Streptomyces rapamycinicus
Strain	NRRL 5491
Entry name	Rapamycin
Contig	X86780
Start / Stop / Direction	101,085 / 98,467 / - [in whole cluster] 101,085 / 98,467 / - [in contig]
Location	complement(98467..101085) [in whole cluster] complement(98467..101085) [in contig]
Type	CDS
Length	2,619 bp (872 aa)

Annotation

Category	4.1 transcriptional regulator
Product	LuxR family transcriptional activator
Product (GenBank)	regulator of cholesterol oxidase
Gene	orfH rapH
Gene (GenBank)	orfH
EC number
Keyword
Note
Note (GenBank)	putative
Reference	ACC Q54307 PmId [7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995) [8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996) [17468238] Roles of rapH and rapG in positive regulation of rapamycin biosynthesis in Streptomyces hygroscopicus. (J Bacteriol. , 2007) [26608164] Interspecies Complementation of the LuxR Family Pathway-Specific Regulator Involved in Macrolide Biosynthesis. (J Microbiol Biotechnol. , 2016) comment [PMID: 7644502](1995) rapamycin生合成gene clusterの報告。配列解析。 ORF H: putative regulator of cholesterol oxidase --- [PMID: 8635730](1996) PKS flanking genesの配列解析。 N末にcanonical P-loop consensusにマッチするpotential ATP-binding motifを含む。 C末付近にDNA-binding motifを含む。 -- [PMID: 17468238](2007) figの様子を見る限り、ORF H = rapH rapH and rapGの遺伝子産物はそれぞれ、LAL(large ATP-binding regulators of the LuxR family) and AraCという2つの異なるfamilyのranscriptional activatorsと類似性を示す。どちらかの遺伝子の過剰発現はrapamycin生合成においてかなりの増加をもたらし、両者の削除は抗生物質産生を完全に失う。相補実験から、rapamycin生合成のためにRapG regulatorは必須であり、RapH regulatorは支持的であることが示された。rapamycin PKSのpromoterでのrapH and rapG遺伝子産物の直接的効果はreporter systemとしてchalcone synthase gene rppAを使って観察された。 --- [PMID: 26608164](2016) Streptomyces venezuelaeでのrapHの異種性発現はpikromycin産生を拡大し、pikD deletion mutantでは補完することができる。

Sequence

>LuxR family transcriptional activator [regulator of cholesterol oxidase]
MTGRANGRGVVVTITGPIACGKTELLDAAAAKADAITLRAVCSAEEQALPYALIGQLIDN
PALASHALEPACPTLPGEHLSPEAENRLRSDLTRTLLALAAERPVLIGIDESHANALCLL
HLARRVGSARIAMVLTELRRLTPAHSQFQAELLSLGHHREIALRPLSPKHTAELVRAGLG
PDVDEDVLTGLYRATGGNLNLTRGLINDVREAWETGGTGISAGRAYRLAYLGSLYRCGPV
PLRVARVAAVLGQSANTTLVRWISGLNADAVGEATEILTEGGLLHDLRFPHPAARSVVLN
DMSAQERRRLHRSALEVLDDVPVEVVAHHQVGAGLLHGPKAAEIFAKAGQELHVRGELDT
ASDYLQLAHQASDDAVTGMRAEAVAIERRRNPLASSRHLDELTVVARAGLLFPEHTALMI
RWLGVGGRSGEAAGLLASQRPRAVTDQDRAHMRAAEVSLALVSPGTSGPDRRPRPLTPDE
LANLPKAARLCAIADNAVMSALRGRPELAAAEAENVLQHADSAAAGTTALAALTALLYAE
NTDTAQLWADKLVSETGASNEEEAGYAGPRAEAALRRGDLAAAVEAGSTVLDHRRLSTLG
ITAALPLSSAVAAAIRLGETERAEKWLAQPLPQAIQDGLFGLHLLSARGQYSLATGQHES
AYTAFRTCGERMRNWGVDVPGLSLWRVDAAEALLHGRDRDEGRRLVDEQLTRAMGPRSRA
LTLRVQAAYSPPAKRVDLLDEAADLLLSCNDQYERARVLADLSETFSALRHHSRARGLLR
QARHLAAQRGAIPLLRRLGAKPGGPGWLEESGLPQRIKSLTDAERRVASLAAGGQTNRVI
ADQLFVTASTVEQHLTDVSTGSRPPAPAAELV

>LuxR family transcriptional activator [regulator of cholesterol oxidase]
GTGACCGGGCGGGCCAACGGCCGGGGTGTGGTGGTCACCATCACCGGACCGATCGCCTGC
GGCAAGACCGAACTGCTCGACGCAGCCGCCGCGAAGGCCGACGCCATCACGTTACGAGCG
GTCTGCTCCGCGGAGGAACAGGCACTCCCGTACGCCCTGATCGGGCAGCTCATCGACAAC
CCGGCGCTCGCCTCCCACGCGCTGGAGCCGGCCTGCCCGACCCTCCCGGGCGAGCACCTG
TCGCCGGAGGCCGAGAACCGGCTGCGCAGCGACCTCACCCGTACCCTGCTGGCGCTCGCC
GCCGAACGGCCGGTGCTGATCGGCATCGACGAGTCACACGCGAACGCTTTGTGTCTGCTC
CACCTGGCCCGAAGGGTCGGCTCGGCCCGGATCGCCATGGTCCTCACCGAGTTGCGCCGG
CTCACCCCGGCCCACTCACAGTTCCAGGCCGAGCTGCTCAGCCTGGGGCACCACCGCGAG
ATCGCGCTGCGCCCGCTCAGCCCGAAGCACACCGCCGAGCTGGTCCGCGCCGGTCTCGGT
CCCGACGTCGACGAGGACGTGCTCACGGGGTTGTACCGGGCGACCGGCGGCAACCTGAAC
CTCACCCGCGGACTGATCAACGATGTGCGGGAGGCCTGGGAGACGGGAGGGACGGGCATC
AGCGCGGGCCGCGCGTACCGGCTGGCATACCTCGGTTCCCTCTACCGCTGCGGCCCGGTC
CCGTTGCGGGTCGCACGGGTGGCCGCCGTGCTGGGCCAGAGCGCCAACACCACCCTGGTG
CGCTGGATCAGCGGGCTCAACGCGGACGCGGTGGGCGAGGCAACCGAGATCCTCACCGAA
GGCGGCCTGCTGCACGACCTGCGGTTCCCGCACCCGGCGGCCCGTTCGGTGGTACTCAAC
GACATGTCCGCCCAGGAACGACGCCGCCTGCACCGGTCCGCTCTGGAAGTGCTGGACGAC
GTGCCCGTGGAAGTGGTCGCGCACCACCAGGTCGGCGCCGGTCTCCTGCACGGCCCGAAG
GCCGCCGAGATATTCGCCAAGGCCGGCCAGGAGCTGCATGTGCGCGGCGAGTTGGACACC
GCGTCCGACTATCTGCAACTGGCCCACCAGGCCTCCGACGACGCCGTCACCGGGATGCGG
GCCGAGGCCGTGGCGATCGAGCGCCGCCGCAACCCGCTGGCCTCGAGCCGGCACCTCGAC
GAGCTGACCGTCGTCGCCCGTGCCGGGCTGCTCTTCCCCGAGCACACGGCGCTGATGATC
CGCTGGCTGGGCGTCGGCGGGCGGTCCGGCGAGGCAGCCGGGCTGCTGGCCTCGCAGCGC
CCCCGTGCGGTCACCGACCAGGACAGGGCCCATATGCGGGCCGCCGAGGTATCGCTCGCG
CTGGTCAGCCCCGGCACGTCCGGCCCGGACCGGCGGCCGCGTCCGCTCACGCCGGATGAG
CTCGCGAACCTGCCGAAGGCGGCCCGGCTCTGCGCGATCGCCGACAATGCCGTCATGTCG
GCCCTGCGCGGTCGTCCCGAGCTCGCCGCGGCCGAGGCGGAGAACGTCCTGCAGCACGCC
GACTCGGCGGCGGCCGGCACCACCGCCCTCGCCGCGCTGACCGCCTTGCTGTACGCGGAG
AACACCGACACCGCTCAGCTCTGGGCCGACAAGCTGGTCTCCGAGACCGGGGCGTCGAAC
GAGGAGGAGGCGGGCTACGCGGGGCCGCGCGCCGAAGCCGCGTTGCGTCGCGGCGACCTG
GCCGCGGCGGTCGAGGCAGGCAGCACCGTTCTGGACCACCGGCGGCTCTCGACGCTCGGC
ATCACCGCCGCGCTACCGCTGAGCAGCGCGGTGGCCGCCGCCATCCGGCTGGGCGAGACC
GAGCGGGCGGAGAAGTGGCTCGCCCAGCCGCTGCCGCAGGCCATCCAGGACGGCCTGTTC
GGCCTGCACCTGCTCTCGGCGCGCGGCCAGTACAGCCTCGCCACGGGCCAGCACGAGTCG
GCGTACACGGCGTTTCGCACCTGCGGGGAACGTATGCGGAACTGGGGCGTTGACGTGCCG
GGTCTGTCCCTGTGGCGCGTCGACGCCGCCGAGGCGCTGCTGCACGGCCGCGACCGGGAC
GAGGGCCGACGGCTCGTCGACGAGCAACTCACCCGTGCGATGGGACCCCGTTCCCGCGCC
TTGACGCTGCGGGTGCAGGCGGCGTACAGCCCGCCGGCGAAGCGGGTCGACCTGCTCGAT
GAAGCGGCCGACCTGCTGCTCTCCTGCAACGACCAGTACGAGCGGGCACGGGTGCTCGCC
GACCTGAGCGAGACGTTCAGCGCGCTCCGGCACCACAGCCGGGCGCGGGGACTGCTTCGG
CAGGCCCGGCACCTGGCCGCCCAGCGCGGCGCGATACCGCTGCTGCGCCGACTCGGGGCC
AAGCCCGGAGGCCCCGGCTGGCTGGAGGAATCCGGCCTGCCGCAGCGGATCAAGTCGCTG
ACCGACGCGGAGCGGCGGGTGGCGTCGCTGGCCGCCGGCGGACAGACCAACCGCGTGATC
GCCGACCAGCTCTTCGTCACGGCCAGCACGGTGGAGCAGCACCTCACGGACGTCTCCACT
GGGTCAAGGCCGCCAGCACCTGCCGCCGAACTCGTCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000792 Transcription regulator LuxR, C-terminal (Domain) SM00421 HTH_LUXR PF00196 GerE IPR011990 Tetratricopeptide-like helical (Domain) G3DSA:1.25.40.10 TPR-like_helical IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR016032 Signal transduction response regulator, C-terminal effector (Domain) SSF46894 Bipartite_resp_reg_C-effector
SignalP	No significant hit
TMHMM	No significant hit

Rapam_00220 Rapam_00240