Rapam_00240 : CDS information

Location

Organism	Streptomyces rapamycinicus
Strain	NRRL 5491
Entry name	Rapamycin
Contig	X86780
Start / Stop / Direction	101,422 / 102,414 / + [in whole cluster] 101,422 / 102,414 / + [in contig]
Location	101422..102414 [in whole cluster] 101422..102414 [in contig]
Type	CDS
Length	993 bp (330 aa)

Annotation

Category	4.1 transcriptional regulator
Product	AraC family transcriptional activator
Product (GenBank)	helix-turn-helix DNA binding protein
Gene	orfG rapG
Gene (GenBank)	orfG
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q54308 PmId [7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995) [8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996) [17468238] Roles of rapH and rapG in positive regulation of rapamycin biosynthesis in Streptomyces hygroscopicus. (J Bacteriol. , 2007) comment [PMID: 7644502](1995) rapamycin生合成gene clusterの報告。配列解析。 ORF G: helix-turn-helix DNA-binding protein? --- [PMID: 8635730](1996) PKS flanking genesの配列解析。中央部にDNA bindingのためのHTH motifを持つ。 -- [PMID: 17468238](2007) figの様子を見る限り、ORF G = rapG rapH and rapGの遺伝子産物はそれぞれ、LAL(large ATP-binding regulators of the LuxR family) and AraCという2つの異なるfamilyのranscriptional activatorsと類似性を示す。どちらかの遺伝子の過剰発現はrapamycin生合成においてかなりの増加をもたらし、両者の削除は抗生物質産生を完全に失う。相補実験から、rapamycin生合成のためにRapG regulatorは必須であり、RapH regulatorは支持的であることが示された。rapamycin PKSのpromoterでのrapH and rapG遺伝子産物の直接的効果はreporter systemとしてchalcone synthase gene rppAを使うことが観察された。

Sequence

>AraC family transcriptional activator [helix-turn-helix DNA binding protein]
MTNGAGAEADGSRGAFSVDSTVPGAAPQGFFQAFQRGWDAELGDGLPLRSFNRDVTGDFR
VRSRAAKVRDLAFTDLHSMSAIRTAHALGGVEDRVRMYVVKRGAWTLAGSRDRDRHTVAA
GQFLLRHVGQPWRFEAAPHTTVKILLLPPGPLTPLLGNRAVTGPADSAEVRLLVAHANMV
YETMTGLGPAGVQAAHSTLLELAQSVARRRFDDVEPRLAPALAGAAKDLADSHLTDPELS
PTMLARELNVSLRTLQRAFTVAGESLIAYIRHRRLEEARRALIASAGRLSVSELAAHWQF
ADSSHFIRVFKKTYGQTPTEYARSTGLTDS

>AraC family transcriptional activator [helix-turn-helix DNA binding protein]
ATGACCAACGGCGCTGGAGCGGAGGCCGACGGATCGCGCGGGGCGTTCTCCGTGGACTCC
ACCGTCCCGGGCGCGGCACCACAAGGATTCTTCCAGGCCTTCCAGCGCGGGTGGGACGCG
GAGCTCGGCGACGGCTTGCCGCTGCGGAGCTTCAACCGGGATGTGACCGGTGACTTCCGG
GTCAGGAGCCGCGCCGCCAAGGTCCGCGACCTGGCGTTCACCGATCTCCACAGCATGTCG
GCCATCCGGACCGCACACGCGCTGGGCGGTGTGGAGGACCGGGTACGGATGTACGTCGTG
AAACGCGGCGCATGGACGTTGGCAGGTTCGCGCGATCGGGACCGGCACACCGTGGCGGCC
GGGCAGTTCCTCCTCCGGCACGTCGGGCAGCCGTGGCGCTTCGAGGCGGCGCCGCACACG
ACGGTGAAGATCCTTCTGCTGCCTCCCGGGCCGCTCACCCCACTGCTGGGAAACCGGGCC
GTCACCGGGCCCGCGGATTCGGCCGAGGTACGCCTGCTGGTGGCCCACGCGAATATGGTG
TACGAGACCATGACCGGCCTGGGCCCGGCCGGTGTGCAAGCCGCCCACAGCACCCTGCTC
GAACTGGCCCAGTCGGTGGCGAGGCGTCGGTTCGACGATGTGGAGCCCCGGCTGGCGCCC
GCGCTCGCCGGGGCCGCGAAGGACCTCGCGGACAGCCACCTCACCGACCCCGAGCTCTCC
CCCACGATGCTGGCGCGTGAACTCAACGTCTCCTTACGCACCTTGCAGCGGGCGTTCACC
GTGGCGGGAGAGTCGTTGATCGCCTACATCCGTCACCGGCGGCTGGAAGAGGCCCGCCGC
GCTCTCATCGCCTCGGCCGGCAGGCTGAGCGTCTCGGAACTCGCCGCCCACTGGCAGTTC
GCCGACAGCAGCCACTTCATCCGGGTCTTCAAGAAGACCTACGGCCAGACGCCCACCGAG
TACGCCCGCTCAACCGGGCTGACCGACAGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR009057 Homeodomain-like (Domain) G3DSA:1.10.10.60 Homeodomain-rel SSF46689 Homeodomain_like IPR018060 DNA binding HTH domain, AraC-type (Domain) SM00342 HTH_ARAC PS01124 HTH_ARAC_FAMILY_2 IPR018062 HTH domain AraC-type, conserved site (Conserved_site) PS00041 HTH_ARAC_FAMILY_1 IPR020449 Transcription regulator HTH, AraC- type (Family) PR00032 HTHARAC
SignalP	No significant hit
TMHMM	No significant hit

Rapam_00230 Rapam_00250