Rapam_00260 : CDS information

Location

Organism	Streptomyces rapamycinicus
Strain	NRRL 5491
Entry name	Rapamycin
Contig	X86780
Start / Stop / Direction	105,198 / 103,801 / - [in whole cluster] 105,198 / 103,801 / - [in contig]
Location	complement(103801..105198) [in whole cluster] complement(103801..105198) [in contig]
Type	CDS
Length	1,398 bp (465 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)
Gene
Gene (GenBank)	orfE
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q54310 PmId [7644502] The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin. (Proc Natl Acad Sci U S A. , 1995) [8635730] Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. (Gene. , 1996) comment [PMID: 7644502](1995) rapamycin生合成gene clusterの報告。配列解析。 ORF E: ? --- [PMID: 8635730](1996) PKS flanking genesの配列解析。配列DBに一致なし。

Sequence

>hypothetical protein
MSADRVAAQYNEEFRIPGSDLPTVAQHVKDFPTLAPWHGEYLARPLFVAEREMREFADDL
QHLIDTVFSLPDRMFGGDLDAFRSVLGISDGRWEAIRRFGDVPPPQFGRADMYHDGTSHK
LLEFNLGSEAGGWGLASEISRATMEIDAFADFARRNRLGYVHPIRAVVEALRHFAARVAP
GREPVVALLEWRGGLKDYGELWLNFQSALRSAGLDILVAEVSDVHERGGALYLGDTKIDV
VYRSFVPDQIAEEPDGWDLVAPLLRAHRAGETLTWTPLSSNMFANKACMALLSDPAWRRD
LGEDECRLIDRVLPWTRLINTDALKTDLVDECVARREQLILKVNGRGGGVGAVVGWETDD
ATWRRALEDSAAEGCVVQQRVIPRPELVVDPVSGETEEWHAAYGMFYTPHGCAGAFAKVV
PTGSGGIISIAAHAKARSAAVFHQSQLPVWGEVPAVGELPADAPK

>hypothetical protein
ATGTCGGCGGACCGGGTAGCAGCCCAGTACAACGAGGAGTTCCGCATACCGGGCAGTGAC
TTGCCCACGGTCGCCCAGCACGTCAAGGACTTCCCGACGCTCGCCCCCTGGCATGGCGAG
TATTTGGCCAGGCCCCTCTTCGTCGCCGAGCGTGAGATGCGCGAGTTCGCCGATGACCTG
CAGCATCTCATCGACACGGTTTTTTCCTTGCCGGACCGGATGTTCGGCGGCGATCTGGAC
GCATTCCGGTCGGTGCTCGGCATCAGCGACGGCCGCTGGGAAGCGATACGGCGGTTCGGT
GACGTACCGCCACCCCAGTTCGGCCGGGCCGACATGTACCACGACGGCACCTCGCACAAA
CTCCTCGAGTTCAATCTGGGGAGCGAGGCCGGCGGCTGGGGCCTCGCGAGTGAAATCTCC
AGGGCGACGATGGAGATTGACGCCTTCGCCGACTTCGCGCGCCGGAATCGGCTTGGTTAC
GTGCATCCCATTCGCGCCGTGGTGGAGGCGCTGCGCCACTTCGCCGCCCGGGTGGCACCC
GGGCGCGAACCGGTGGTGGCCCTGCTTGAATGGCGTGGGGGTCTGAAGGACTACGGCGAA
CTCTGGCTCAACTTCCAGTCCGCACTGCGCTCGGCGGGCCTGGACATCCTGGTGGCCGAG
GTCTCCGACGTGCACGAACGCGGCGGGGCGCTCTACCTCGGGGACACGAAGATCGACGTG
GTCTACCGCTCCTTCGTCCCGGACCAGATCGCCGAGGAGCCCGACGGCTGGGACCTGGTC
GCGCCCCTGCTGCGTGCGCACCGGGCGGGCGAGACGCTGACATGGACCCCGCTTTCCAGC
AACATGTTCGCCAACAAGGCCTGTATGGCGCTGCTTTCGGATCCGGCGTGGCGGCGCGAC
CTCGGCGAGGACGAGTGCCGGCTCATCGACCGGGTGCTGCCCTGGACCCGGCTGATCAAC
ACGGACGCGCTCAAGACCGACCTCGTCGACGAATGCGTGGCGCGGCGCGAGCAGTTGATC
CTGAAGGTCAACGGGCGCGGCGGGGGAGTGGGCGCCGTGGTGGGCTGGGAAACGGACGAC
GCCACTTGGCGGCGGGCTCTGGAGGACTCCGCCGCCGAAGGATGTGTCGTACAACAGCGG
GTGATCCCCCGGCCCGAGCTCGTGGTGGACCCGGTATCCGGGGAAACCGAGGAATGGCAT
GCGGCCTATGGCATGTTCTACACCCCGCACGGCTGTGCCGGAGCGTTCGCCAAGGTGGTG
CCGACCGGTTCGGGCGGCATCATTTCCATCGCGGCGCACGCCAAGGCGCGCAGCGCGGCC
GTCTTCCATCAGAGCCAGCTGCCCGTCTGGGGCGAAGTGCCTGCCGTGGGCGAATTGCCC
GCCGATGCGCCGAAGTAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005494 Glutathionylspermidine synthase, pre-ATP-grasp domain (Domain) PF03738 GSP_synth
SignalP	No significant hit
TMHMM	No significant hit

Rapam_00250 Rapam_00270