Rifam_00160 : CDS information

Location

Organism	Amycolatopsis mediterranei
Strain	S699
Entry name	Rifamycin
Contig	AF040570
Start / Stop / Direction	14,341 / 15,309 / + [in whole cluster] 14,341 / 15,309 / + [in contig]
Location	14341..15309 [in whole cluster] 14341..15309 [in contig]
Type	CDS
Length	969 bp (322 aa)

Annotation

Category	5.1 general function
Product	putative oxidoreductase
Product (GenBank)	RifS
Gene
Gene (GenBank)	rifS
EC number
Keyword
Note
Note (GenBank)	putative NADH-dependent dehydrogenase
Reference	ACC Q7BUE3 PmId [9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998) comment ansamycin系抗生物質rifamycinの生合成gene clusterの報告。 orf0の上流にある3つのORFは機能不明。このORFはそれに含まれる。
Related Reference	ACC Q2I8V6 PmId [16461673] Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis. (Appl Environ Microbiol. , 2006) comment 310th(Q2I8V6) 30%, 9e-15 Ensifer adhaerens (Sinorhizobium morelense)_afr 1,5-anhydro-D-fructose reductase(EC 1.1.1.292) UniProt entry name変更有り：Q2I8V6_9RHIZ→AFR_ENSAD --- Sinorhizobium morelense(UniProtでは E.adhaerens) S-30.7.5株由来の1,5-anhydro-D-fructose(AF) reductaseの単離およびcharacterization. nativeだけでなく、His(6)-tagged recombinant(E.coliにて発現)も作製。 AFから1,5-anhydro-D-mannitol(AM)への触媒活性を確認。 -->NADPH-dependent monomeric reductase
Related Reference	ACC Q9KWL3 PmId [11073908] Genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4,5-cleavage pathway in Sphingomonas paucimobilis SYK-6. (J Bacteriol. , 2000) comment 372nd(Q9KWL3) 29%, 4e-14 Pseudomonas paucimobilis _ligC 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase リグニン分解系である中間体Protocatechuate(PCA)の4,5-cleavage産物である4-carboxy-2-hydroxymuconate-6-semialdehyde(CHMS)を2-pyrone-4,6-dicarboxylate(PDC)にする。 NADP依存、homodimer。mutantはvanillate上で生育できない。遺伝子ligA(Protocatechuate 4,5-dioxygenase alpha chain,P22635)-ligB(beta chain,P22636)-ligCと並んでいる。

Sequence

>putative oxidoreductase [RifS]
MALIGAGLIARLHLEAWLGAGAAVRVYSDDGRSRELAAEFGAKAAGSLEEALDGADAVDI
CTPTASHHEIALTAIAAGVGVVCEKPLAASAEEAEEIVTAAERAGVRLYAAHDVRFAAPY
ARLHELVASGRLGEGALGRFSFSAYHPRPWTGHASARSGGILTDQLLHGADLAHWVFGDV
VRVHACYQGDIATPAPEGAVATGTAVLTHASGAISQVVSRWTATPRPPVRVAFHVSGTGG
SVSYDSEWPQEVRVVDGGAGNFAYGGPSVFDTEMREFATAFAGGPEPRIGAKDALAAVRI
IHAAAESAWTGRAVELPVRGAA

>putative oxidoreductase [RifS]
GTGGCCCTGATCGGTGCGGGGCTCATCGCCCGGCTGCACCTGGAAGCGTGGCTCGGCGCC
GGCGCCGCCGTGCGGGTGTACTCCGACGACGGCCGGAGCCGCGAGCTGGCGGCGGAGTTC
GGCGCGAAGGCCGCCGGGTCCCTGGAAGAGGCGCTCGACGGCGCCGACGCCGTCGACATC
TGCACGCCGACCGCGAGCCACCACGAGATCGCCCTGACCGCCATCGCCGCCGGTGTCGGC
GTCGTGTGCGAGAAGCCGCTGGCGGCCAGCGCCGAAGAGGCCGAAGAGATCGTCACCGCC
GCCGAACGGGCCGGGGTGCGGCTCTACGCCGCCCACGACGTGCGGTTCGCGGCGCCCTAC
GCGCGATTGCACGAGCTCGTTGCGAGCGGGCGGCTCGGGGAGGGTGCCCTCGGCCGGTTC
TCCTTCTCCGCCTACCACCCGCGGCCCTGGACCGGGCACGCCTCCGCGCGGTCGGGCGGC
ATCCTCACCGACCAGCTGCTGCACGGCGCCGACCTCGCCCACTGGGTCTTCGGGGACGTC
GTCCGCGTGCACGCCTGCTACCAGGGCGACATCGCCACCCCGGCGCCCGAAGGGGCCGTC
GCGACCGGGACCGCCGTGCTCACCCACGCGAGCGGCGCCATCAGCCAGGTGGTGAGCCGG
TGGACGGCCACGCCGCGGCCGCCGGTGCGGGTGGCCTTCCACGTCTCGGGTACCGGCGGG
TCCGTCAGCTACGACTCCGAGTGGCCGCAGGAGGTCCGGGTCGTCGACGGCGGCGCCGGG
AACTTCGCTTACGGCGGGCCGTCGGTGTTCGACACCGAAATGCGGGAGTTCGCCACGGCT
TTCGCGGGTGGACCCGAGCCGAGGATCGGCGCGAAGGACGCGCTGGCCGCCGTGCGGATC
ATCCACGCCGCCGCCGAGTCCGCGTGGACCGGGCGGGCCGTCGAACTGCCGGTGAGGGGA
GCAGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000683 Oxidoreductase, N-terminal (Domain) PF01408 GFO_IDH_MocA IPR004104 Oxidoreductase, C-terminal (Domain) PF02894 GFO_IDH_MocA_C IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Eukaryota Bacteria, Gram-positive
TMHMM	No significant hit

Rifam_00140 Rifam_00170