Rifam_00190 Rifam_00190   Rifam_00210 Rifam_00210

Rifam_00200 : CDS information

close this sectionLocation

Organism
StrainS699
Entry nameRifamycin
Contig
Start / Stop / Direction16,510 / 17,700 / + [in whole cluster]
16,510 / 17,700 / + [in contig]
Location16510..17700 [in whole cluster]
16510..17700 [in contig]
TypeCDS
Length1,191 bp (396 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)putative cytochrome P450-like protein
Gene
Gene (GenBank)
EC number
Keyword
  • hydroxylate
Note
Note (GenBank)
  • ORF0
Reference
ACC
PmId
[9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998)
comment
ansamycin系抗生物質rifamycinの生合成gene clusterの報告。
Orf0(397aa): cytochrome P450 monooxygenase

orf0とPKSをコードするrifA-Eが近位であることから、Orf0はproansamycin Xのnaphthoquinone形成のときかその前に、C-1をhydroxylateすると考えられる。
Related Reference
ACC
A6YRR3
PmId
[19376895] Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14. (Appl Environ Microbiol. , 2009)
comment
25th, id45%, 6e-85
Streptomyces virginiae
Cytochrome P450 monooxygenase 105

cytochrome P450 monooxygenase, FcpCを同定した文献。
FcpCがdiosgenoneのC-25のhydroxylationを担い、isonuatigenoneに変換する機能を持つことがin vitroで示されている。

この論文で同定されたfcpC(1191nt) and fcpD(243nt)遺伝子配列のGenBank accession numberはEF646279であると記載あり。
よってこのentry=FcpCで合っている。

close this sectionSequence

selected fasta
>putative cytochrome P450 [putative cytochrome P450-like protein]
MTDAISFEVPWDRTDKFDPPAVFDSLREERPLAKMVYPDGHVGWIVSSYELVREVLSDLR
FSHSCEVGHFPVTHQGQVIPTHPLIPGMFIHMDPPEHTRYRKLLTGEFTVRRASRLIPRA
EAVAAEQIEVMRAKGAPADVVMDFAKPLVLRMLGELVGLPYEERDRYVPAVTLLHDAEAD
PAEAAAAYEVAGKFFDEVIERRRQRPQDDLISSLVTEDLTQEELRNIVTLLLFAGYETTE
GALATGVFALLHHTDQLAALRAEPEKLDAAIEELLRYLTVNQYHTYRTALEDVKLEGELI
KKGDTVTVSLPAANRDPAKFGCPAELDIERDTSGHVAFGFGIHQCLGQNLARIELRAGFT
ALLRAFPELRLAVPADEVPLRLKGSVFSVKKLPVSW
selected fasta
>putative cytochrome P450 [putative cytochrome P450-like protein]
ATGACCGACGCAATATCCTTCGAGGTGCCGTGGGACCGGACCGACAAGTTCGACCCGCCC
GCGGTGTTCGACTCTCTGCGCGAAGAACGTCCGCTCGCGAAGATGGTTTACCCGGATGGG
CACGTCGGCTGGATCGTTTCCAGCTACGAGCTGGTCCGCGAGGTCCTCAGCGACCTGCGG
TTCAGCCACAGCTGCGAAGTCGGCCACTTCCCGGTGACCCACCAGGGCCAGGTCATCCCG
ACCCACCCGCTGATCCCCGGCATGTTCATCCACATGGACCCGCCCGAGCACACGCGCTAC
CGCAAGCTGCTGACCGGCGAGTTCACCGTCCGCCGCGCCAGCAGGCTGATCCCGCGGGCC
GAGGCCGTGGCCGCCGAGCAGATCGAGGTCATGCGGGCCAAGGGCGCCCCCGCGGACGTG
GTCATGGACTTCGCCAAGCCGCTGGTGCTGCGGATGCTGGGCGAGCTCGTCGGCCTGCCC
TACGAGGAACGCGACCGGTACGTGCCCGCGGTGACCCTCCTGCACGACGCCGAAGCGGAC
CCGGCCGAGGCCGCGGCCGCCTACGAGGTGGCCGGGAAGTTCTTCGACGAGGTCATCGAG
CGCCGCCGGCAGCGGCCCCAGGACGACCTCATCAGCTCGCTCGTCACCGAGGACCTGACC
CAGGAGGAGCTGCGCAACATCGTCACCCTGCTGCTGTTCGCCGGGTACGAGACCACCGAG
GGCGCGCTCGCCACCGGCGTCTTCGCGCTGCTGCACCACACCGATCAGCTGGCGGCACTG
CGCGCGGAGCCGGAAAAGCTCGACGCCGCGATCGAAGAGCTGCTGCGCTACCTGACCGTC
AACCAGTACCACACCTACCGCACCGCGCTGGAGGACGTGAAGCTGGAGGGCGAGCTGATC
AAGAAGGGCGACACGGTGACGGTGTCGCTGCCCGCGGCCAACCGCGACCCGGCCAAGTTC
GGCTGTCCCGCGGAGCTCGACATCGAGCGGGACACCTCCGGCCACGTCGCGTTCGGCTTC
GGCATCCACCAGTGCCTGGGCCAGAACCTGGCGCGCATCGAGCTGCGGGCCGGCTTCACG
GCGCTCCTGCGGGCGTTCCCCGAGCTCCGGCTGGCCGTCCCGGCCGACGAGGTTCCGCTG
CGGCTGAAGGGTTCCGTCTTCTCGGTGAAGAAGCTGCCCGTCTCCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
[234-251] 1.1e-08 PR00385 [269-280] 1.1e-08 PR00385 [336-345] 1.1e-08 PR00385 [345-356] 1.1e-08 PR00385
PR00385   P450
[11-396] 8.7e-101 G3DSA:1.10.630.10
G3DSA:1.10.630.10   no description
[189-392] 2e-27 PF00067
PF00067   p450
[17-396] 1.5e-93 SSF48264
SSF48264   Cytochrome P450
IPR002397 Cytochrome P450, B-class (Family)
[92-103] 1.8e-52 PR00359 [139-155] 1.8e-52 PR00359 [156-171] 1.8e-52 PR00359 [193-215] 1.8e-52 PR00359 [269-280] 1.8e-52 PR00359 [287-314] 1.8e-52 PR00359 [315-330] 1.8e-52 PR00359 [336-345] 1.8e-52 PR00359 [345-356] 1.8e-52 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
[338-347] PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit
Rifam_00190 Rifam_00190   Rifam_00210 Rifam_00210
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