Rifam_00330 Rifam_00330   Rifam_00350 Rifam_00350

Rifam_00340 : CDS information

close this sectionLocation

Organism
StrainS699
Entry nameRifamycin
Contig
Start / Stop / Direction75,365 / 76,276 / + [in whole cluster]
75,365 / 76,276 / + [in contig]
Location75365..76276 [in whole cluster]
75365..76276 [in contig]
TypeCDS
Length912 bp (303 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.2 modification addition of starter unit
Productkanosamine kinase
Product (GenBank)RifN
Gene
Gene (GenBank)rifN
EC number
Keyword
  • AHBA
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
  • kanosamine kinase
Reference
ACC
PmId
[9512878] Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699. (Chem Biol. , 1998)
[11278540] Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699. (J Biol Chem. , 2001)
[12207505] Characterization of the early stage aminoshikimate pathway in the formation of 3-amino-5-hydroxybenzoic acid: the RifN protein specifically converts kanosamine into kanosamine 6-phosphate. (J Am Chem Soc. , 2002)
comment
[PMID: 9512878](1998)
ansamycin系抗生物質rifamycinの生合成gene clusterの報告。
RifN(303aa): kinase

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[PMID: 11278540](2001)
rifamycinと関連ansamycinsのPKS starter 3-amino-5-hydroxybenzoic acid (AHBA)の生合成経路について調査。関連遺伝子の不活化、異種性発現、中間体を基質とした反応確認など。

どちらもRifN: kinase

rifNは、PKSのstarter unitであるAHBAの合成に必要十分な7genes(rifG, H, J, K, L, M, N)のひとつだが、rifH, G, Jと異なりshikimate pathway関連遺伝子には似ていない。

rifN mutantの実験から、AHBA生合成の早期段階で作用し、aminoDAHP形成か、それより前で関与することが示唆されている。

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[PMID: 12207505](2002)
rifNについての実験論文。

E.coliでHis6 fusion proteinとして過剰発現、精製したRifNの基質特異性を様々な糖で確認。kanosamineのみが反応し、この反応生成物をNMRなどで解析したところ、kanosamine 6-phosphateと同定された。

よって、RifNはkanosamine kinase
kanosamine → kanosamine 6-phosphate

close this sectionSequence

selected fasta
>kanosamine kinase [RifN]
MGTPYHLGIDVGGTKVAFRVESGSACIEETSFSWGARHSAEDDLAQLAGHVARLRERIGT
PLEAVGVAMPGTVGADGRVATWPSRPEWTGVDLKTALHSLFPEAAVAWADDGDLGALAES
RASGCENLLYIGIGTGIGGGLVLGGVPCPGLGRGSFEIGHVIVEMGGVRCVCGRRGCLQA
LASGPATLRRASLLRGADVTYYRLQRALRNGEPWAADALEGSTRALAAAVTGVQELVHPD
RVLIGGGFAAGIPEIVPSVSGFLADLVRQGQAPLPVEPAALGGLSSLRGAVALAGLVAAG
EVP
selected fasta
>kanosamine kinase [RifN]
GTGGGCACCCCGTACCACCTCGGCATCGACGTGGGAGGCACGAAAGTCGCCTTCCGCGTC
GAGTCCGGCTCCGCCTGTATCGAGGAAACCTCGTTCAGCTGGGGAGCCCGGCACAGTGCC
GAGGACGACCTCGCCCAGCTGGCCGGCCACGTGGCCCGGCTGCGGGAGCGCATCGGCACG
CCGCTGGAGGCGGTCGGGGTCGCCATGCCCGGCACCGTCGGCGCGGACGGCCGGGTCGCG
ACCTGGCCCAGCCGCCCGGAGTGGACCGGGGTGGACCTGAAAACGGCGTTGCACTCGCTG
TTCCCCGAAGCGGCGGTCGCCTGGGCGGACGACGGCGATCTCGGCGCGCTCGCCGAGTCC
CGGGCGTCGGGCTGCGAGAACCTGCTGTACATCGGGATCGGCACCGGCATCGGCGGCGGC
CTGGTCCTGGGTGGCGTGCCGTGCCCCGGCCTCGGCCGCGGTTCGTTCGAGATCGGCCAC
GTGATCGTGGAGATGGGCGGCGTCCGCTGTGTGTGCGGCCGCCGCGGCTGCCTGCAGGCA
CTCGCCTCCGGCCCGGCGACCCTCCGCCGCGCGAGCCTGTTGCGCGGAGCGGACGTCACC
TACTACCGGCTGCAGCGGGCGCTGCGCAACGGAGAGCCGTGGGCGGCCGACGCGCTGGAA
GGAAGCACCCGGGCGCTGGCGGCCGCGGTGACCGGCGTCCAGGAGCTGGTCCACCCGGAC
CGCGTGCTGATCGGCGGCGGGTTCGCGGCGGGGATCCCGGAGATCGTGCCGTCGGTGTCG
GGGTTCCTGGCGGATCTGGTGCGACAGGGTCAGGCGCCGCTGCCGGTGGAACCGGCCGCG
TTGGGCGGGCTGTCGTCGTTGCGGGGAGCGGTGGCCCTCGCCGGGCTGGTGGCGGCGGGG
GAAGTGCCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000600 ROK (Family)
[8-186] 6.7e-24 PF00480
PF00480   ROK
SignalP No significant hit
TMHMM No significant hit
Rifam_00330 Rifam_00330   Rifam_00350 Rifam_00350
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