Rubra_00050 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	5,629 / 4,625 / - [in whole cluster] 5,629 / 4,625 / - [in contig]
Location	complement(4625..5629) [in whole cluster] complement(4625..5629) [in contig]
Type	CDS
Length	1,005 bp (334 aa)

Annotation

Category	5.1 general function
Product	putative F420-dependent glucose-6-phosphate dehydrogenase
Product (GenBank)	hypothetical protein
Gene
Gene (GenBank)
EC number	1.1.98.2
Keyword
Note
Note (GenBank)	ORF2
Reference	ACC Q2PC85 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。 ORF2(334aa): Glucose-6-phosphate dehydrogenase Rubradirin生合成における機能未知。
Related Reference	ACC O68447 PmId [8631674] Purification of a novel coenzyme F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis. (J Bacteriol. , 1996) [9555906] Molecular analysis of the gene encoding F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis. (J Bacteriol. , 1998) [20075070] Glucose 6-phosphate accumulation in mycobacteria: implications for a novel F420-dependent anti-oxidant defense system. (J Biol Chem. , 2010) comment 38th, id68%, 1e-126 Mycobacterium smegmatis_fgd F420-dependent glucose-6-phosphate dehydrogenase(EC 1.1.98.2) --- [PMID: 8631674, 9555906] 電子受容体がNAD,NADPではなくて、F420(8-ヒドロキシ-5-デアザフラビン環を含む補酵素。メタン発酵に用いられる)。 NADPでは不活性であること確かめている。基質Glc-6P特異性。 NADP-dependent typeも持っている。お互いの遺伝子は相同性ない。どちらをどうやって使い分けているかは、まだ不明。 --- [PMID: 20075070](2010) abstract 6つのMycobacterium sp.における細胞内のG6Pレベルは、E.coliやBacillus megateriumより異常に高い(約17-130倍）。fgd mutantは、酸化ストレスを誘導する細胞外のplumbagin and menadioneを還元する能力に欠陥がある。mycobacteriaにおいて細胞内のG6Pは還元力の源として役立ち、 mycobacteria-specific Fgd-F(420) systemと共に、酸化ストレスに対する防御的役割を果たすことが示唆される。

Sequence

>putative F420-dependent glucose-6-phosphate dehydrogenase [hypothetical protein]
MGLKIGYKASAEQFAPRELVEYAVSAEGHGLDSVWISDHLQPWRHEGGHAPFSLSWLSAV
GERTERVQLGTSVLTATFRYNPAVVAHASATLGVLYPGRIALGIGSGEALNEVAVARITW
PDFKERFARLREAVELIRRLWTEDRVTSEGQYHQTTSVSLYDRPQTPVPIYIAAGGPVMA
KYVGRKSDGFICTSGKGMELYTEKLLPAVEAGADQEGRDHNAIDKMIEIKLSYDPDPDQA
LENCRFWAPLSLTPEQKAGVEDPVKLEKAADELPIGQVAKRWIVASTPDEAITQIKQYVD
AGFNHLVFHGPGHDQERFLKTFAEQVLPGLRGLG

>putative F420-dependent glucose-6-phosphate dehydrogenase [hypothetical protein]
ATGGGACTGAAGATCGGTTACAAGGCATCGGCCGAGCAGTTCGCCCCGCGCGAGCTCGTC
GAGTACGCGGTCAGCGCGGAGGGGCACGGCCTGGACAGTGTCTGGATCAGCGATCACCTG
CAGCCCTGGCGGCACGAGGGCGGCCACGCGCCATTCTCCCTGTCCTGGCTCAGTGCCGTC
GGGGAGCGCACCGAGCGGGTCCAGCTCGGCACCAGCGTGCTGACCGCCACGTTCCGCTAC
AACCCGGCCGTGGTGGCGCACGCGTCCGCCACCCTCGGAGTGCTGTACCCCGGCCGGATC
GCGCTCGGCATAGGCAGCGGTGAGGCCCTCAACGAGGTCGCGGTCGCGCGCATCACCTGG
CCCGATTTCAAGGAGCGGTTCGCCCGCCTGCGCGAGGCCGTGGAGCTGATCCGCCGGCTG
TGGACGGAAGACCGCGTCACATCCGAGGGGCAGTACCACCAGACCACCAGCGTGTCCCTC
TACGACCGGCCCCAAACGCCCGTCCCCATCTACATCGCCGCCGGGGGCCCCGTCATGGCC
AAGTACGTCGGCCGCAAGAGCGACGGCTTCATCTGCACCAGCGGCAAGGGCATGGAGCTG
TACACCGAGAAGCTCCTCCCGGCCGTGGAGGCGGGCGCGGACCAGGAGGGCCGTGACCAC
AACGCGATCGACAAAATGATCGAGATAAAGCTCTCCTACGACCCGGACCCGGACCAAGCG
CTGGAGAACTGCCGGTTCTGGGCGCCGCTTTCGCTAACTCCCGAGCAGAAGGCCGGAGTC
GAGGACCCGGTGAAGTTGGAGAAGGCCGCCGATGAACTGCCCATCGGGCAGGTTGCCAAG
CGCTGGATCGTCGCCTCCACCCCGGACGAGGCGATCACCCAGATCAAGCAGTACGTCGAC
GCCGGGTTCAACCACTTGGTCTTCCACGGCCCCGGGCACGACCAGGAGCGCTTCCTCAAG
ACCTTCGCCGAGCAGGTCCTCCCCGGACTGCGCGGCCTCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR011251 Luciferase-like domain (Domain) PF00296 Bac_luciferase G3DSA:3.20.20.30 Luciferase_like SSF51679 Luciferase_like IPR019944 Glucose-6-phosphate dehydrogenase, F420-dependent (Family) TIGR03554 F420_G6P_DH IPR019945 Glucose-6-phosphate dehydrogenase-related, F420-dependent (Family) TIGR03557 F420_G6P_family
SignalP	No significant hit
TMHMM	No significant hit

Rubra_00040 Rubra_00060