Rubra_00060 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	5,775 / 7,058 / + [in whole cluster] 5,775 / 7,058 / + [in contig]
Location	5775..7058 [in whole cluster] 5775..7058 [in contig]
Type	CDS
Length	1,284 bp (427 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	cytochrome P450
Gene
Gene (GenBank)	rubP6
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q2PC84 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。 Table 1でRubP3となっているがRubP6に相当。 RubP3(427aa): Cytochrome P450 機能は不明だが、アグリコンの形成後、Morpholine ring形成に関与していると推測される。
Related Reference	ACC P48635 PmId [8416893] Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. (J Bacteriol. , 1993) [7849045] ( , ) comment Blast 28th, id41%, 3e-65 Saccharopolyspora erythraea_eryK Erythromycin C-12 hydroxylase(EC 1.14.13.154) --- [PMID: 8416893](1993) Saccharopolyspora erythraea (Streptomyces erythraeus)において、P450 monooxygenase familyに属するEryKが、erythromycin生合成の最終反応の１つであるmacrolactone ringのC-12 hydroxylationに関与していることをeryK変異体を作成して調べている。 --- [PMID: 7849045](1995) 不溶性封入体としてE.coliでEryKを過剰産生し、可溶化、再折りたたみ、active holo-EryK再構築をした。動態確認で、erythromycin Bより1200-1900倍もerythromycin Dを基質として好むことが分かった。
Related Reference	ACC Q2PC42 NITE Rubra_00480 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment 2nd, id71%, 1e-164 Rubradirin生合成cluster内homolog RubP2: Cytochrome P450 本文に詳細記載なし

Sequence

>putative cytochrome P450 [cytochrome P450]
MSGRFGPVLRWLASSAESGRTREVSFMSVKQSERILSNFLAYLDGLRERGAVHFDDKIKA
WHVLDYHDVLQVLTDSAAFSSDVAPLAPKQEDFDLFKKGNFVRADDPVHRRLRGLVSRAF
TPKMIAGLEPRITEVTLQLLDDADAGGDQWDLIEQLGYPLPFIVIAEMLGIPVSDRAFVR
RLSDTFFEVHNVDPEQSLDSLGESAVNNVAPLLRELNTHLLEFVRRRRKNPGDDLTSKLL
EVEVDGVRLDDEETVGFLGL-LLAGHVTATSTLGNTVLALHENPEVWTQLRADPALIPAA
VEESMRYRPPFPRLGRQATKDVEIGGQKIPAGAFVLAWLTTANRDDRVFADPDRFDIHRA
PNPHLTFGKGMHFCLRGALARLEVGIALRLLMERYSDIRVRDDLPVNLRNPWAMIGVNQL
PIEVRKG

>putative cytochrome P450 [cytochrome P450]
ATGTCCGGCCGGTTCGGTCCGGTTCTACGGTGGCTGGCGAGTAGCGCGGAATCGGGCCGT
ACGAGAGAAGTGAGTTTCATGAGCGTGAAACAATCCGAACGGATACTGTCCAACTTTCTC
GCCTATCTCGACGGGCTGCGCGAGCGCGGAGCCGTGCACTTCGACGACAAGATCAAGGCG
TGGCACGTACTCGATTACCACGACGTCCTGCAGGTGCTGACCGACTCGGCGGCCTTCTCG
TCCGACGTCGCCCCGCTGGCTCCCAAGCAGGAAGACTTCGATCTGTTCAAGAAGGGCAAC
TTCGTCCGGGCGGACGATCCGGTGCACCGGCGGCTGCGCGGCCTGGTCAGCCGCGCGTTC
ACCCCGAAGATGATCGCCGGACTTGAACCCCGGATCACCGAGGTGACCTTGCAGCTGCTC
GACGACGCGGACGCCGGCGGCGACCAGTGGGACCTGATCGAGCAGCTGGGCTACCCGCTG
CCGTTCATCGTCATCGCCGAGATGCTCGGCATCCCGGTCAGCGACCGCGCGTTCGTCCGG
CGGCTGTCCGACACCTTCTTCGAGGTGCACAACGTCGATCCGGAACAGTCGCTGGATTCC
CTCGGGGAGAGCGCGGTGAACAATGTCGCGCCGCTGCTGCGCGAGTTGAACACCCACCTG
CTGGAGTTCGTCCGCCGCCGCCGCAAGAACCCGGGCGACGACCTCACCAGCAAGCTTCTG
GAGGTCGAGGTCGACGGTGTGCGGCTGGACGACGAGGAGACCGTCGGCTTCCTCGGCCTG
CTNCTGCTCGCCGGCCACGTCACCGCGACCTCCACCCTGGGCAACACGGTTCTCGCGTTG
CACGAGAACCCCGAGGTGTGGACGCAGCTGCGGGCCGATCCCGCGCTGATTCCGGCCGCG
GTCGAGGAGTCGATGCGCTACCGGCCTCCGTTCCCCCGTCTCGGGCGGCAGGCGACGAAG
GACGTCGAGATCGGCGGGCAGAAGATCCCGGCCGGCGCCTTCGTCCTGGCGTGGCTGACC
ACCGCGAACCGCGATGACCGCGTCTTCGCCGACCCGGACCGGTTCGACATCCACCGGGCC
CCCAACCCGCACCTGACGTTCGGCAAGGGCATGCACTTCTGCCTTCGGGGCGCCCTGGCG
CGGCTCGAGGTGGGGATCGCCCTGCGCCTTCTGATGGAGCGGTACAGCGACATCCGCGTA
CGCGACGACCTACCGGTCAACCTGCGCAACCCCTGGGCGATGATCGGCGTCAACCAGCTG
CCCATCGAGGTCCGCAAGGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) G3DSA:1.10.630.10 no description PF00067 p450 SSF48264 Cytochrome P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450
SignalP	Eukaryota
TMHMM	No significant hit

Rubra_00050 Rubra_00070