Rubra_00080 Rubra_00080   Rubra_00100 Rubra_00100

Rubra_00090 : CDS information

close this sectionLocation

Organism
StrainNRRL 3061 (=NBRC 14000)
Entry nameRubradirin
Contig
Start / Stop / Direction24,447 / 24,671 / + [in whole cluster]
24,447 / 24,671 / + [in contig]
Location24447..24671 [in whole cluster]
24447..24671 [in contig]
TypeCDS
Length225 bp (74 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
Productputative translation initiation factor IF-1
Product (GenBank)putative Translation initiation factor
Gene
Gene (GenBank)rubR3
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
comment
Rubradirin生合成clusterの報告。
RubR3(74aa): Translation initiation factor

RubradirinはTranslationを阻害することで抗生物質として機能するので、このORFはResistanceに関与していると推測される。
Related Reference
ACC
P20458
PmId
[2496109] ( , )
comment
Blast 77th, id74%, 3e-21
Bacillus subtilis_infA
Translation initiation factor IF-1

IF1-B-S13-S11-rpoH-L17 gene clusterのクローニング。promoterの解析。

close this sectionSequence

selected fasta
>putative translation initiation factor IF-1 [putative Translation initiation factor]
MAKVNRGIEVVGTIVESLKDAKFWVELENGHKVLAHISGKLRKHYIKILPQDTVTVELSP
YDLTRGRITFRHRM
selected fasta
>putative translation initiation factor IF-1 [putative Translation initiation factor]
GTGGCAAAAGTAAATCGAGGTATCGAGGTTGTCGGTACCATCGTCGAGTCCCTGAAAGAC
GCCAAGTTCTGGGTTGAACTCGAGAACGGTCACAAGGTACTCGCACACATCAGCGGGAAG
CTCCGCAAGCACTACATCAAGATCCTGCCGCAGGACACGGTGACGGTCGAGTTGAGCCCG
TATGACCTGACCCGTGGCCGGATCACCTTCCGGCACCGGATGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004368 Translation initiation factor IF-1 (Family)
[7-72] 8.70000000000003e-34 TIGR00008
TIGR00008   InfA
IPR006196 RNA-binding domain, S1, IF1 type (Domain)
[8-71] 7.7e-23 PF01176
PF01176   eIF-1a
[9-73] PS50832
PS50832   S1_IF1_TYPE
IPR012340 Nucleic acid-binding, OB-fold (Domain)
[6-72] 3.89999999999997e-33 G3DSA:2.40.50.140
G3DSA:2.40.50.140   OB_NA_bd_sub
IPR016027 Nucleic acid-binding, OB-fold-like (Domain)
[2-72] 1.39999892049878e-25 SSF50249
SSF50249   Nucleic_acid_OB
IPR022967 RNA-binding domain, S1 (Domain)
[5-73] 1.29999924468179e-09 SM00316
SM00316   S1
SignalP
[1-35] 0.079 Signal
Eukaryota   
TMHMM No significant hit
Rubra_00080 Rubra_00080   Rubra_00100 Rubra_00100
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