Rubra_00220 Rubra_00220   Rubra_00240 Rubra_00240

Rubra_00230 : CDS information

close this sectionLocation

Organism
StrainNRRL 3061 (=NBRC 14000)
Entry nameRubradirin
Contig
Start / Stop / Direction42,933 / 41,812 / - [in whole cluster]
42,933 / 41,812 / - [in contig]
Locationcomplement(41812..42933) [in whole cluster]
complement(41812..42933) [in contig]
TypeCDS
Length1,122 bp (373 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductNDP-hexose C-3 aminotransferase
Product (GenBank)putative aminotransferase
Gene
Gene (GenBank)rubN4
EC number2.6.1.-
Keyword
  • D-rubranitrose
Note
Note (GenBank)
Reference
ACC
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
[16614891] Functional characterization of ketoreductase (rubN6) and aminotransferase (rubN4) genes in the gene cluster of Streptomyces achromogenes var. rubradiris. (Biotechnol Lett. , 2006)
comment
Rubradirin生合成clusterの報告。

配列比較からのProposed function
RubN4(373aa): Aminotransferase (sugar)

RubN1-N8はdTDP-D-rubranitroseの合成に推定的に関与していることが配列解析で明らかになった。

---
上記論文でrubN4, N6のrefとして挙げられているもの。
[PMID: 16614891](2006) abstract

rubN6 and rubN4をHis-tagged fusion proteinsとしてE.coliで異種性に発現。
それぞれのdTDP-glucose 4-ketoreductase and dTDP-glucose 3-aminotransferaseの機能性を、in vitro enzyme assayによって検証。

close this sectionSequence

selected fasta
>NDP-hexose C-3 aminotransferase [putative aminotransferase]
MTIRVWDYLPEYHEEREEVLAAVTKVFESGQLILGPSVAGFEAEFAAYHGAAHCAGVDNG
TNAVKLGLQALGVGKGDEVITVANTAAPTVVAIDGTGAKPVFVDVREDDFLMDTDQVAAA
IGPRTRALVPVHLYGQCVDMAPLRRLADRHSLAILEDCAQAHGARHHGRLAGTMGDVAAF
SFYPTKVLGAYGDGGAVITGDEDTDRNVRRLRYYGMEDRYFVERVPGHNSRLDEVQAEIL
RGKLRRLDAYISKRRDIARLYTKALGDLAEPGGLVLPTVSFGNEHVYYLYVVRHPRRDEI
IERLRAYGIALNISYPWPVHTMAGFAHLGCARGSLPVTEKLAGEVFSLPMYPSLPLDRQQ
TVIQALREVLAGF
selected fasta
>NDP-hexose C-3 aminotransferase [putative aminotransferase]
GTGACGATCCGGGTATGGGACTACCTGCCGGAGTACCACGAGGAACGCGAGGAGGTACTC
GCGGCGGTGACGAAGGTGTTCGAGTCCGGCCAGCTGATACTGGGTCCCAGCGTGGCCGGG
TTCGAGGCCGAGTTCGCGGCGTACCACGGCGCCGCCCACTGCGCCGGCGTCGACAACGGC
ACCAACGCGGTCAAGCTGGGGCTGCAGGCGCTCGGCGTGGGCAAGGGCGATGAGGTGATC
ACCGTCGCCAACACCGCCGCGCCGACCGTGGTGGCCATCGACGGGACGGGCGCGAAGCCG
GTCTTCGTGGATGTACGCGAGGACGACTTCCTCATGGACACCGACCAGGTCGCGGCCGCG
ATCGGCCCGCGCACACGGGCACTGGTCCCCGTGCACCTCTATGGCCAGTGCGTCGACATG
GCCCCGCTGCGCCGGCTCGCCGACCGCCACAGCCTGGCCATCCTCGAGGACTGCGCCCAA
GCGCACGGCGCGCGCCACCACGGCCGGCTGGCAGGCACGATGGGCGACGTCGCCGCCTTC
TCCTTCTATCCCACGAAGGTTCTCGGCGCGTACGGCGACGGTGGCGCCGTGATCACCGGC
GACGAGGACACCGACCGCAACGTCCGGCGCCTGCGCTACTACGGCATGGAAGACCGCTAC
TTCGTGGAGCGGGTTCCCGGACACAACAGCCGCCTCGACGAGGTACAGGCCGAGATTCTG
CGGGGCAAGCTGCGCCGGCTCGACGCGTACATCTCCAAGCGCCGCGACATCGCGCGGCTC
TACACCAAGGCGCTGGGCGACCTCGCCGAACCGGGCGGCCTCGTCCTGCCGACGGTCTCC
TTTGGCAATGAGCACGTGTACTACCTGTACGTCGTTCGGCACCCCAGGCGGGACGAGATC
ATCGAACGGCTGCGCGCGTACGGCATCGCGCTGAACATCAGCTACCCCTGGCCGGTGCAC
ACGATGGCCGGATTCGCGCACCTCGGATGCGCCAGGGGCTCGCTGCCGGTCACCGAGAAA
CTGGCAGGCGAGGTGTTTTCGCTGCCGATGTACCCGTCGCTGCCGTTGGACCGGCAGCAG
ACGGTGATCCAGGCCCTGCGCGAGGTGCTGGCCGGATTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
[1-373] 1.3000049540733e-94 PIRSF000390
PIRSF000390   PLP_StrS
[15-367] 4.59999999999996e-117 PF01041
PF01041   DegT_DnrJ_EryC1
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[16-246] 1.20000000000001e-77 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[247-370] 1.6e-36 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[6-373] 1.3000049540733e-105 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit
Rubra_00220 Rubra_00220   Rubra_00240 Rubra_00240
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