Rubra_00220 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	41,782 / 40,547 / - [in whole cluster] 41,782 / 40,547 / - [in contig]
Location	complement(40547..41782) [in whole cluster] complement(40547..41782) [in contig]
Type	CDS
Length	1,236 bp (411 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-3 C-methyltransferase
Product (GenBank)	hypothetical protein
Gene
Gene (GenBank)	rubN5
EC number	2.1.1.-
Keyword	D-rubranitrose
Note
Note (GenBank)
Reference	ACC Q2PC68 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。配列比較からのProposed function RubN5(411aa): Hypothetical, protein 本文中では3-C-methyltransferase RubN1-N8はdTDP-D-rubranitroseの合成に推定的に関与していることが配列解析で明らかになった。 everninomicin producerであるMicromonospora carbonaceaにおけるL-evernitroseの生合成に関与する3-C-methyltransferasesにAAid67% chloroeremomycin producerであるAmycolatopsis orientalisにおけるL-epivancosamineの生合成に関与する3-C-methyltransferasesにAAid62% tylosinでL-mycarose生合成と関連する3-C-methyltransferase TylC3にAAid26%しかないのは、D-rubranitrose と L-mycaroseで3-C-methyl基の向きが立体化学的に逆であるせいかもしれない。
Related Reference	ACC P96561 NITE Chlor_00150 PmId [11035791] Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. (Proc Natl Acad Sci U S A. , 2000) comment BLAST id67% Amycolatopsis orientalis PCZA361.22 ORF14(EvaC)に相当するentry. -- [PMID: 11035791](2000) EvaC(ORF14)を含むdTDP-L-epivancosamine合成に関連する5つのタンパクをE.coliで発現、精製。これらと基質を組み合わせて反応させ、産物のmass spectrometry and NMRによって各酵素活性、dTDP-L-epivancosamine生合成経路を同定している。 EvaC(ORF14)は、dTDP-3-amino-2,3,6-trideoxy-D-threo-hexopyranos-4-uloseのC-3にequatorialなmethyl基を形成する。 C-3 methyl基をaxialにするのは、この後の反応が関連している。

Sequence

>putative NDP-hexose C-3 C-methyltransferase [hypothetical protein]
MPRERATVTCRVCDGTVHEFLDLGRQPLSDAFREPDCDTEEFFFRLAVGQCESCTMVQLT
ESVPRDKMFHEDYPYHSSGSAVMREHFAGVARRFLATELAAPDPFIVEMGCNDGVMLSTI
HQAGVRHLGFEPSGRVAELARTKGVRVLTEFFEDSTAARIREAEGPAQVIFAANTMCHIP
YPNSVFRGVDALLAPDGVFVFEDPYLGDLVEKTSFDQIYDEHFFLFSAHSVRAMAEHHGF
ELVDVERLPVHGGEVRYTLARTGGRTPSAAVADLLAEEDARGLSDMETLRGFAAGVMRVR
DDLVALLRRLRTEGSSVVAYGATAKSATVTNFCDIGPELVSYVCDSTPAKQHRMTPGKHI
PVRPPTAFRDPYPDYALLFAWNHADEIMANEQEFRRSGGRWISYVPNVRVL

>putative NDP-hexose C-3 C-methyltransferase [hypothetical protein]
ATGCCGCGAGAACGGGCGACCGTCACCTGCAGAGTGTGCGACGGGACAGTCCACGAGTTC
CTCGACCTCGGCCGTCAGCCGCTGTCCGACGCGTTCCGGGAACCGGACTGCGACACCGAG
GAGTTCTTCTTCCGGCTCGCCGTCGGGCAATGCGAGTCGTGCACGATGGTGCAGCTCACC
GAGTCGGTACCGCGGGACAAGATGTTCCACGAGGACTACCCGTACCACTCGTCCGGGTCG
GCGGTGATGCGGGAGCACTTCGCGGGTGTCGCACGACGGTTCCTCGCCACCGAGTTGGCC
GCGCCGGACCCGTTCATCGTCGAGATGGGCTGCAACGACGGCGTCATGCTGAGCACGATC
CACCAGGCAGGCGTACGCCACCTGGGCTTCGAACCTTCCGGGCGGGTGGCCGAGCTGGCC
CGCACCAAGGGCGTCCGGGTCCTCACCGAGTTCTTCGAGGACTCGACAGCGGCGCGGATC
CGCGAGGCGGAAGGGCCCGCCCAGGTCATCTTCGCGGCGAACACCATGTGCCACATCCCG
TACCCGAACTCGGTCTTCCGCGGAGTCGACGCACTGCTGGCACCGGACGGCGTGTTCGTG
TTCGAGGACCCCTACCTCGGCGACCTGGTGGAGAAGACGTCCTTCGACCAGATCTACGAC
GAGCACTTCTTCCTGTTCAGCGCCCACTCGGTGCGGGCCATGGCCGAGCACCACGGATTC
GAGCTGGTCGACGTGGAACGCCTGCCGGTGCACGGCGGCGAGGTCCGGTACACGCTCGCC
CGGACCGGCGGACGCACACCCAGCGCCGCCGTCGCCGACCTGCTCGCCGAGGAGGACGCC
CGCGGGCTGTCCGACATGGAGACGCTGCGTGGCTTCGCCGCCGGCGTGATGCGCGTGCGC
GACGACCTGGTGGCACTGCTGCGACGGCTGCGCACCGAAGGATCCAGCGTGGTCGCCTAC
GGCGCCACGGCGAAAAGCGCCACAGTCACCAACTTCTGCGACATCGGGCCCGAACTCGTC
TCCTACGTGTGTGACAGCACGCCCGCCAAGCAGCACCGCATGACGCCCGGCAAGCACATC
CCCGTACGCCCGCCGACCGCGTTCCGCGACCCGTACCCGGACTACGCGCTGCTGTTCGCG
TGGAACCACGCCGACGAAATCATGGCGAACGAACAGGAGTTCCGGCGCTCCGGCGGCCGA
TGGATCAGCTATGTGCCGAACGTCCGGGTGCTCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR013630 Methyltransferase putative zinc binding domain (Domain) PF08421 Methyltransf_13 IPR013691 C-methyltransferase (Domain) PF08484 Methyltransf_14
SignalP	No significant hit
TMHMM	No significant hit

Rubra_00210 Rubra_00230