Rubra_00320 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	53,814 / 53,158 / - [in whole cluster] 53,814 / 53,158 / - [in contig]
Location	complement(53158..53814) [in whole cluster] complement(53158..53814) [in contig]
Type	CDS
Length	657 bp (218 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	putative F420 dependent NADP reductase
Gene
Gene (GenBank)	rubF7
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q2PC58 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。 Table1>配列比較からの推定機能 RubP7(218aa): F420-dependent NADP reductase Table1ではRubP7とされているが、RubF7が正しいと思われる。 rubransarolのmorpholine環形成のための様式は不明なままだが、rubF4 to rubF7 and rubP3 genesがこの過程で役割を果たすかもしれない。

Sequence

>hypothetical protein [putative F420 dependent NADP reductase]
MTGFLIPDAPLPSARHLRLAIVGPDDCWAAALVEAFATVGRTVERLSLEELAPARTRAHQ
ADVAFLTIRTSQMPSLVPRLGDALKGVVTVICTTSLQHDESGYFMQPVHGGSATGLASEL
LPGSRVVGAFQQFGPSHLALAGMGVFETDVPVIGDDREACDVVEELIDEFRGLEPVYAGG
LKTAAAIEGLAALVGEATAGIGRPVGFRLSSGGIKILD

>hypothetical protein [putative F420 dependent NADP reductase]
ATGACCGGATTCCTCATCCCGGACGCGCCGCTGCCTTCGGCACGGCACCTCAGGCTGGCG
ATCGTCGGGCCTGACGACTGCTGGGCTGCCGCTCTGGTGGAGGCGTTCGCCACGGTAGGA
CGCACCGTCGAGCGGCTCTCCCTTGAGGAGTTGGCGCCGGCCAGAACGCGAGCACACCAG
GCCGACGTGGCGTTCCTGACCATCCGCACCTCCCAGATGCCGTCGCTGGTCCCCCGGTTG
GGCGACGCGCTGAAGGGCGTCGTCACGGTGATATGCACGACATCGCTTCAGCATGACGAG
AGCGGCTACTTCATGCAGCCCGTACACGGCGGGAGCGCCACCGGACTGGCAAGTGAGCTT
CTTCCCGGCTCCCGGGTCGTCGGAGCCTTTCAGCAGTTCGGTCCCAGCCATCTGGCACTT
GCCGGCATGGGCGTTTTCGAGACCGACGTCCCCGTCATCGGAGACGACCGCGAGGCCTGT
GACGTCGTGGAGGAACTGATCGACGAGTTCCGCGGACTTGAGCCGGTCTACGCCGGCGGA
CTGAAAACCGCCGCCGCAATCGAGGGTCTGGCGGCGTTGGTAGGAGAGGCCACCGCCGGC
ATCGGACGGCCGGTCGGATTCCGTCTCAGCTCAGGAGGCATCAAAATCCTCGACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	No significant hit
TMHMM	No significant hit

Rubra_00310 Rubra_00330