Rubra_00310 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	52,987 / 51,398 / - [in whole cluster] 52,987 / 51,398 / - [in contig]
Location	complement(51398..52987) [in whole cluster] complement(51398..52987) [in contig]
Type	CDS
Length	1,590 bp (529 aa)

Annotation

Category	5.2 function unknown
Product	aminocoumarin--carboxylic acid ligase amide synthetase
Product (GenBank)	putative aminocoumarin ligase
Gene
Gene (GenBank)	rubF6
EC number
Keyword	inactive
Note
Note (GenBank)
Reference	ACC Q2PC59 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) [21448870] Adenylate-forming enzymes of rubradirin biosynthesis: RubC1 is a bifunctional enzyme with aminocoumarin acyl ligase and tyrosine-activating domains. (Chembiochem. , 2011) comment [PMID: 18080113](2008) Rubradirin生合成clusterの報告。配列比較からのProposed function RubF6(529aa): Aminocoumarin ligase rubransarolのmorpholine環形成のための様式は不明なままだが、rubF4 to rubF7 and rubP3 genesがこの過程で役割を果たすかもしれない。 --- [PMID:21448870](2011) aminocoumarin生合成、結合に関連するputative acyladenylate-forming enzymesの候補、Orf4, RubF6, RubC1を発現、精製して調査している。 RubF6 (56 kDa)は不活性だが、site-directed mutagenesisにより活性型酵素へ変換されうる。 RubF6はOrf4に高い相同性があることから、これらは遺伝子重複に由来し、その後一方が不活性型へ変異された可能性がある。
Related Reference	ACC Q9L9F6 PmId [10801869] Cloning, overexpression, and purification of novobiocic acid synthetase from Streptomyces spheroides NCIMB 11891. (J Biol Chem. , 2000) comment 12th(Q9L9F6) 35%, 2e-65 Streptomyces sphaeroides_novL NovL Novobiocic acid synthetaseをコードするnovLをE.coliで発現、精製、活性測定。 3-dimethylallyl-4-hydroxybenzoic acid (ring A of novobiocin) と 3-amino-4,7-dihydroxy-8-methyl coumarin (ring B of novobiocin) の間のアミド結合の形成をATP-dependent reactionで触媒した。また、ring A and ATPからのacyl adenylateの形成がATP-PP(i) exchange assayによって実証された。すなわちNovLは、acyl adenylateのようなring Aの活性化と、引き続くacyl基のring Bのamino基への移動の両方を触媒した。

Sequence

>aminocoumarin--carboxylic acid ligase [putative aminocoumarin ligase]
MSTTHVHYVQQLLTHFDADPDRLAVVSGEVPFSAGELADAVRRAAAAMGRHGVGRGDVVC
ILTEPNTAATLILQWAANLVGATAAHMRWVDPAGPEDELRAELQRALAADTGVRMLAVDP
ANEARARELLVNATGRPVLAVLGAGQPDTVDVSAGCGDGVGPCADITDGDVAVITQTRLP
SGRNGLCWTFGVRNDMLSAAPSLPSTAGASATGPTTLLITAPLIHTDVFTAEDTLVTGGM
VVLHPGFDAAAVLRAIAQHRVGRLMLGAPQLDALAEHPDRAATDLSSLTELIYTGSPGAP
LKLRKAREIFGPVLIQVYGTTETGVLTMLPPGDHDDLRACSSVGRPVDPGALSIRHPDTG
AVLPVGEVGEVCAVPRWPTAGYWHEPALTAALVRDGWVRTGDLGHLDTDGYLHLTGRLAN
MMKVKGIRIHPEQVEKVLRQAPGVSQAAVCGVEDADRVEHIYAAVVPEPGADPDPRELRR
HVAEALSDTYVPRLIDIRRKLPTTGWGKPDRVRLRADARAALTRPAPQA

>aminocoumarin--carboxylic acid ligase [putative aminocoumarin ligase]
ATGAGTACCACGCATGTGCATTATGTTCAGCAGCTGCTGACTCATTTCGATGCCGATCCG
GATCGCCTTGCCGTGGTCAGCGGGGAAGTCCCCTTCTCGGCCGGGGAGCTGGCCGACGCC
GTTCGCCGGGCCGCGGCCGCGATGGGCCGCCACGGCGTCGGCCGCGGTGACGTCGTCTGC
ATCCTGACCGAGCCGAACACCGCGGCCACGCTGATCCTGCAGTGGGCGGCCAACCTGGTG
GGGGCGACCGCCGCGCACATGCGCTGGGTGGACCCGGCCGGGCCCGAGGACGAGTTGCGC
GCGGAGCTGCAGCGCGCCCTGGCCGCGGATACCGGTGTCCGGATGCTGGCGGTCGACCCG
GCCAACGAGGCGCGGGCGCGGGAGCTGCTGGTGAACGCGACCGGTCGGCCGGTCCTGGCC
GTACTGGGCGCAGGGCAGCCGGACACGGTGGATGTGAGCGCCGGTTGCGGTGACGGAGTG
GGCCCGTGCGCGGACATCACCGACGGCGACGTCGCGGTGATCACCCAGACCAGGCTGCCG
AGCGGACGGAACGGCCTCTGCTGGACCTTCGGGGTCAGGAACGACATGCTCTCGGCCGCA
CCGTCCCTGCCCTCCACGGCCGGCGCGAGCGCGACCGGCCCGACGACCCTCCTGATCACC
GCACCGCTCATCCACACCGACGTGTTCACCGCGGAGGACACGCTCGTCACCGGCGGCATG
GTCGTCCTGCATCCCGGCTTCGACGCGGCGGCCGTGCTGCGTGCGATCGCGCAGCACCGC
GTCGGCCGGCTCATGCTCGGCGCTCCGCAACTGGACGCACTGGCCGAGCACCCGGACCGG
GCGGCCACCGACCTGTCCAGCCTGACCGAGCTGATCTACACCGGCAGCCCCGGGGCACCG
CTCAAGCTGCGCAAGGCCCGGGAAATCTTCGGCCCCGTACTGATCCAGGTCTACGGCACG
ACCGAGACCGGAGTGCTCACCATGCTCCCCCCCGGCGACCACGACGACCTGCGCGCCTGC
TCCAGCGTGGGCCGGCCGGTCGACCCCGGGGCGCTCAGCATCCGGCACCCCGACACCGGC
GCGGTGCTGCCCGTCGGCGAGGTCGGCGAGGTCTGCGCGGTTCCACGCTGGCCCACGGCC
GGCTACTGGCACGAGCCGGCGCTCACCGCGGCACTCGTCCGCGACGGCTGGGTGCGCACC
GGCGACCTCGGGCACCTCGACACCGACGGCTACCTGCACCTGACCGGCCGGCTCGCGAAC
ATGATGAAGGTCAAGGGAATCCGCATCCACCCCGAGCAGGTGGAGAAGGTGCTCAGGCAG
GCCCCGGGCGTGTCCCAGGCCGCCGTATGCGGGGTCGAGGACGCCGACCGGGTGGAGCAC
ATCTACGCCGCCGTCGTGCCCGAGCCCGGCGCCGACCCGGATCCCCGGGAACTGCGCCGT
CACGTCGCCGAGGCACTGTCCGACACCTACGTCCCCCGCCTGATCGACATTCGCCGGAAA
CTGCCGACGACCGGCTGGGGCAAACCCGATCGGGTCCGGCTGCGGGCCGACGCCCGCGCG
GCACTGACCCGGCCCGCCCCCCAGGCCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000873 AMP-dependent synthetase/ligase (Domain) PF00501 AMP-binding
SignalP	No significant hit
TMHMM	No significant hit

Rubra_00300 Rubra_00320