Rubra_00410 Rubra_00410   Rubra_00430 Rubra_00430

Rubra_00420 : CDS information

close this sectionLocation

Organism
StrainNRRL 3061 (=NBRC 14000)
Entry nameRubradirin
Contig
Start / Stop / Direction82,829 / 83,578 / + [in whole cluster]
82,829 / 83,578 / + [in contig]
Location82829..83578 [in whole cluster]
82829..83578 [in contig]
TypeCDS
Length750 bp (249 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative beta-hydroxytyrosyl-S-PCP dehydrogenase
Product (GenBank)putative oxyacyl-(acyl carrier protein) reductase
Gene
Gene (GenBank)rubC3
EC number
Keyword
  • aminocoumarin moiety
Note
Note (GenBank)
Reference
ACC
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
comment
Rubradirin生合成clusterの報告。
rubC1-C5はAMC and DHDP biosynthesisに関連する。

Table1>配列比較からの推定機能
RubC3(249aa): Oxoacyl-(acyl carrier protein) reductase

rubC1-C4はaminocoumarin生合成に関連するものに似ている。
RubC3はNovJに似ている。NovJはNovKと共にhydroxytyrosine→keto-tyrosineへの変換にゆるく結び付けられている。けれどNovK homologueはrub gene clusterに検出されなかった。

novJを参照しているが、Ref論文はRelated Referenceに登録したものより古い、同じ人たちの2000年のもの。
Related Reference
ACC
Q9L9F8
PmId
[16171397] NovJ/NovK catalyze benzylic oxidation of a beta-hydroxyl tyrosyl-S-pantetheinyl enzyme during aminocoumarin ring formation in novobiocin biosynthesis. (Biochemistry. , 2005)
comment
357th(Q9L9F8) 47%, 8e-49
Streptomyces sphaeroides_novJ
NovJ

J2K2 heterotetramerとしてE.coliで発現、精製。RP-HPLCで酵素反応産物を解析。
NovJはNovKとheterotetramerを形成し、beta-OH-Tyr-S-NovHのbeta-OH基を酸化してbeta-keto-Tyr-S-NovHを生成する。この反応には電子受容体としてNADP+が使用される。
NADP-dependent oxidoreductasesとしてのactive sitesがNovJでは保存されているが、NovKではない。NovJのY164A mutantは活性が50倍減る。

--
この残基はRubC3で保存されている(Y150)。

close this sectionSequence

selected fasta
>putative beta-hydroxytyrosyl-S-PCP dehydrogenase [putative oxyacyl-(acyl carrier protein) reductase]
MNRVAIVTGSARGIGAAVAGRLVGDGIDVAVLDVDVTAAQSTAAGLVRAGGRAGAIGVDV
SDADAVSTAVDEVVDRFGPPCILVNNAGITRDNMVFSLSPADWDLVINVNLRGTFLMTHA
VAEHMAKLGWGRVVNLSSIAAVGNPGQANYSAAKAGIEGLTKTLALELGPLNVTVNAVAP
GYVATEMTAALAAQAGRTAEEHQAQAAATIPLRRVGRPEDIADVVAFLASDHASFVSGQI
IHVAGGPVC
selected fasta
>putative beta-hydroxytyrosyl-S-PCP dehydrogenase [putative oxyacyl-(acyl carrier protein) reductase]
GTGAACCGCGTCGCCATCGTCACCGGATCGGCCCGGGGCATCGGTGCCGCGGTGGCCGGC
AGGCTGGTCGGGGACGGCATCGACGTCGCCGTCCTCGACGTGGACGTGACGGCCGCGCAG
AGCACGGCCGCCGGCCTGGTCCGGGCGGGCGGCCGCGCCGGGGCCATCGGGGTCGACGTG
AGCGACGCCGACGCGGTCAGCACGGCGGTCGACGAAGTCGTCGACCGGTTCGGGCCGCCC
TGCATCCTGGTCAACAACGCCGGCATCACCCGGGACAACATGGTCTTCTCCCTCTCACCG
GCCGACTGGGACCTGGTCATCAACGTGAACCTGCGCGGCACATTCCTGATGACGCACGCG
GTCGCCGAGCACATGGCCAAGCTCGGCTGGGGCCGCGTGGTGAACCTGTCGAGCATCGCT
GCGGTCGGCAACCCCGGCCAGGCCAACTATTCGGCGGCGAAAGCCGGAATCGAGGGCCTG
ACCAAGACGCTGGCCCTGGAACTCGGTCCGCTGAACGTCACAGTCAACGCCGTGGCGCCC
GGCTACGTGGCCACCGAGATGACCGCGGCCCTGGCGGCGCAGGCCGGCCGGACGGCCGAG
GAACACCAGGCGCAGGCCGCGGCGACGATCCCGCTGCGCCGGGTCGGCCGGCCGGAGGAC
ATCGCCGACGTCGTGGCCTTCCTGGCCAGCGACCATGCCTCGTTCGTGTCCGGCCAGATC
ATCCATGTCGCCGGCGGACCGGTCTGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[4-168] 3.29999999999997e-35 PF00106
PF00106   adh_short
[78-89] 3.0999994589937e-12 PR00080 [131-139] 3.0999994589937e-12 PR00080 [150-169] 3.0999994589937e-12 PR00080
PR00080   SDRFAMILY
IPR002347 Glucose/ribitol dehydrogenase (Family)
[4-21] 2.39999798157265e-46 PR00081 [78-89] 2.39999798157265e-46 PR00081 [125-141] 2.39999798157265e-46 PR00081 [150-169] 2.39999798157265e-46 PR00081 [171-188] 2.39999798157265e-46 PR00081 [211-231] 2.39999798157265e-46 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[2-247] 2.79999999999994e-84 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[137-165] PS00061
PS00061   ADH_SHORT
SignalP
[1-19] 0.456 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Rubra_00410 Rubra_00410   Rubra_00430 Rubra_00430
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