Rubra_00410 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	81,603 / 82,832 / + [in whole cluster] 81,603 / 82,832 / + [in contig]
Location	81603..82832 [in whole cluster] 81603..82832 [in contig]
Type	CDS
Length	1,230 bp (409 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	putative cytochrome P450
Gene
Gene (GenBank)	rubC2
EC number
Keyword	aminocoumarin moiety hydroxylate
Note
Note (GenBank)
Reference	ACC Q2PC49 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。 rubC1-C5はAMC and DHDP biosynthesisに関連する。 Table1>配列比較からの推定機能 RubC2(409aa): Cytochrome P450 rubC1-C4はaminocoumarin生合成に関連するものに似ている。 RubC2はtyrosyl-NovH→hydroxytyrosyl-NovHの変換で機能するNovIに似ている。
Related Reference	ACC Q9L9F9 PmId [11325587] Coumarin formation in novobiocin biosynthesis: beta-hydroxylation of the aminoacyl enzyme tyrosyl-S-NovH by a cytochrome P450 NovI. (Chem Biol. , 2001) comment 5th(Q9L9F9), 65%, 1e-153 Streptomyces sphaeroides_novI NovI NovIは45 kDaのヘムタンパクであり、tyrosyl-S-NovH acyl enzymeに特異性を持ったcytochrome P450-type monooxygenaseとして機能する。その産物であるbeta-OH-tyrosyl-S-NovHは、radioactive [3H]beta-OH-Tyrのalkaline releaseとHPLC解析と、mass spectrometryによって検出された。

Sequence

>putative cytochrome P450 [putative cytochrome P450]
MTRPVVSPELLDSLDLADPVLHAENELSEVWRRLRQERSHYWHPARGDRPGFWVFTPYAD
AMAVYRDKEHFTTEGGNALATLLAGGDSASGSMLAVTDGDKHTQLRNVLNAAFSPRRLKT
IRTAIRRTIDDLIRAAIDKGECDFVHDVSANVPLGAICGLMDVPEADRRYLLGLTSHAWS
SDYADAPPQESWQAKSEILLYFADLAHTRRGSDADDVVSLLANCRIGGGPLSDADLMSNC
YGLMIGGDETGRHAISGGLQALIEWPDQWRALKEGTVGLDTAANEVLRWTVPSLHGGRTA
SGDTTVNGQPVKKGDIVSVWISSANRDPEVFADPDRFLLDRRPNKHLTFAFGSHLCLGHT
LARIEVEEILDSLRTLVSCAEQTGPERWIYSSILHGMSSLPVTLTPEPA

>putative cytochrome P450 [putative cytochrome P450]
ATGACCCGCCCCGTAGTCAGCCCTGAGCTCCTGGATTCGCTCGACCTCGCCGACCCGGTG
CTGCACGCCGAGAACGAACTCTCCGAGGTGTGGCGCCGGCTCCGGCAGGAGCGGTCCCAC
TACTGGCATCCGGCGCGGGGGGACCGTCCCGGCTTCTGGGTCTTCACCCCGTACGCCGAC
GCGATGGCGGTCTACCGGGACAAGGAACACTTCACCACCGAAGGCGGCAACGCGCTGGCG
ACCCTGCTGGCCGGCGGCGACTCGGCGTCCGGCTCGATGCTCGCGGTGACCGACGGCGAC
AAGCACACCCAGCTGCGCAACGTGCTCAACGCGGCGTTCTCCCCTCGCCGGCTCAAGACC
ATCCGCACGGCGATCCGGCGGACCATCGACGACCTGATCCGGGCGGCGATCGACAAAGGC
GAGTGCGATTTCGTCCACGACGTGTCCGCGAACGTCCCGCTGGGTGCGATCTGTGGCCTC
ATGGACGTGCCCGAGGCGGATCGCCGCTATCTGCTCGGGCTCACCTCGCACGCGTGGAGT
TCCGACTACGCGGACGCCCCGCCGCAGGAGAGCTGGCAGGCCAAGAGCGAGATCCTGCTC
TACTTCGCCGACCTGGCGCACACCCGGCGCGGCAGTGACGCCGACGACGTGGTCAGCCTG
CTGGCCAACTGCCGCATCGGGGGCGGGCCGTTGAGCGACGCCGACCTGATGTCCAACTGC
TACGGGCTGATGATCGGCGGCGACGAGACCGGACGGCATGCCATCTCCGGCGGTCTGCAG
GCACTGATCGAGTGGCCGGACCAGTGGCGGGCGCTCAAGGAGGGCACCGTCGGCCTCGAC
ACCGCCGCCAACGAGGTGCTGCGCTGGACCGTGCCGTCCCTGCACGGCGGACGCACGGCG
TCCGGGGACACCACGGTGAACGGCCAGCCGGTCAAGAAGGGCGACATCGTCAGCGTCTGG
ATCTCCTCGGCCAACCGGGACCCCGAGGTCTTCGCCGACCCGGACCGCTTCCTCCTCGAC
CGCAGGCCGAACAAGCACCTGACCTTCGCCTTCGGCTCGCACCTCTGCCTGGGGCACACG
CTGGCACGCATCGAAGTGGAGGAAATCCTCGACTCGTTGCGCACGCTCGTGTCGTGCGCG
GAACAGACCGGGCCGGAGCGGTGGATCTACTCCAGCATCCTGCACGGCATGAGCTCCCTG
CCGGTCACGCTGACGCCCGAGCCGGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) SSF48264 Cytochrome_P450 G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450
SignalP	Bacteria, Gram-negative
TMHMM	No significant hit

Rubra_00400 Rubra_00420