Rubra_00490 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	91,154 / 89,958 / - [in whole cluster] 91,154 / 89,958 / - [in contig]
Location	complement(89958..91154) [in whole cluster] complement(89958..91154) [in contig]
Type	CDS
Length	1,197 bp (398 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	glycosyltransferase
Product (GenBank)	putative glycosyltransferase
Gene
Gene (GenBank)	rubG2
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q2PC41 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。 Table1>配列比較からの推定機能 RubG2(398aa): Glycosyltransferase 2つのglycosyltransferase analogues RubG1 and G2がrub gene clusterで検出された。 RubG2 mutantの産物をHPLCでwildと比較したところRubradirinは検出されず、LC-MS解析でrubransarolのピークが示されたことから、RubG2の機能はrubradirin生合成におけるglycosylTFである。

Sequence

>glycosyltransferase [putative glycosyltransferase]
MKILFSSHPGYGHTHPLLPLALAARQAGHDVLYATGERFHPLLRGLGFCTVPAGMPIRDA
FAAALAGTDGTNGQVPPEEWLEPAVRVLNEILPRRFAADLLPVLRAQRPDLVVYQAGNPG
AGLAAHRAGIPAVCHANARAEAIDPADRWQRRGMQLLRAVAAENGVELPEGQYLLGDRYL
DIYPDPLQETSFVDRPERMLLRPVPFNESGELPQIVLAGRKRPLVYLTLGTVLGTAAALR
AAVDGLSALDAEVVVAAGPNISLADFPALPAHVHLKQWVPQAELLSLADLAVHHGGSATT
LGAAGSGVPQLFLPIGFDGFLNAGAVAAAGAGRRLLPEGVQRWEDRRRCDVVSAAAVTEA
AGGLLADPGARQAAKAIAEEIATMPSPADIAARLPEFC

>glycosyltransferase [putative glycosyltransferase]
ATGAAGATCCTTTTCTCCAGCCATCCCGGTTATGGCCACACCCACCCTTTGCTGCCGCTG
GCCCTAGCCGCCCGGCAGGCCGGCCATGACGTCCTGTATGCCACGGGTGAGAGATTCCAC
CCGCTGCTGCGAGGGCTCGGATTTTGTACCGTGCCGGCCGGGATGCCGATCCGGGATGCC
TTCGCCGCCGCCTTAGCAGGGACGGACGGGACGAACGGGCAAGTGCCGCCGGAGGAATGG
CTCGAGCCCGCGGTCCGGGTGTTAAACGAGATACTGCCCCGCCGGTTCGCCGCCGACCTG
CTGCCCGTGCTGCGGGCACAGCGGCCCGACCTGGTGGTGTACCAGGCCGGTAACCCGGGA
GCCGGGCTCGCGGCACACCGCGCGGGCATTCCCGCCGTATGCCACGCCAACGCCCGGGCG
GAGGCGATCGACCCGGCAGACCGGTGGCAGCGGCGCGGCATGCAGCTGCTGCGCGCGGTC
GCTGCCGAGAACGGTGTGGAACTGCCCGAGGGGCAGTACCTCCTCGGTGACCGGTACCTG
GACATCTATCCGGACCCGCTGCAGGAAACGTCGTTTGTGGACCGTCCGGAACGGATGTTG
CTGCGCCCGGTGCCCTTCAACGAATCCGGTGAGCTGCCGCAGATCGTCCTGGCCGGACGG
AAACGGCCGCTGGTCTACCTGACGCTGGGCACCGTTCTCGGCACCGCTGCCGCCCTGCGC
GCCGCCGTCGACGGGCTGTCCGCCCTGGACGCCGAGGTGGTGGTGGCGGCCGGGCCGAAC
ATTTCGCTGGCCGATTTCCCTGCCCTGCCCGCTCATGTGCACCTCAAGCAGTGGGTGCCG
CAGGCGGAACTGCTGTCACTGGCCGATCTCGCGGTGCACCACGGCGGTTCCGCAACCACC
CTCGGCGCGGCCGGTTCCGGGGTACCGCAACTGTTCCTGCCCATCGGATTCGACGGGTTC
CTCAACGCCGGCGCGGTCGCGGCGGCCGGTGCCGGCAGGCGGCTGCTGCCCGAGGGGGTC
CAACGATGGGAGGACCGGCGCCGCTGCGATGTCGTCTCCGCCGCGGCCGTCACCGAGGCA
GCCGGCGGGCTGCTGGCCGATCCCGGGGCCAGACAGGCGGCCAAGGCCATCGCCGAGGAG
ATCGCCACCATGCCGAGCCCCGCGGACATCGCGGCCCGGCTCCCCGAGTTCTGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR010610 Domain of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Rubra_00480 Rubra_00500