Rubra_00480 : CDS information

Location

Organism	Streptomyces achromogenes var. rubradiris
Strain	NRRL 3061 (=NBRC 14000)
Entry name	Rubradirin
Contig	AJ871581
Start / Stop / Direction	89,824 / 88,622 / - [in whole cluster] 89,824 / 88,622 / - [in contig]
Location	complement(88622..89824) [in whole cluster] complement(88622..89824) [in contig]
Type	CDS
Length	1,203 bp (400 aa)

Annotation

Category	3.3 modification reduction
Product	putative cytochrome P450
Product (GenBank)	putative cytochrome P450
Gene
Gene (GenBank)	rubP2
EC number
Keyword
Note
Note (GenBank)
Reference	ACC Q2PC42 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment Rubradirin生合成clusterの報告。 Table1>配列比較からの推定機能 RubP2(400aa): Cytochrome P450 本文に詳細記載なし。
Related Reference	ACC Q2PC84 NITE Rubra_00060 PmId [18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008) comment 2nd, id71%, 1e-159 Rubradirin生合成cluster内homolog Table1>配列比較からの推定機能 RubP3(427aa): Cytochrome P450 機能は不明だが、アグリコンの形成後、Morpholine ring形成に関与していると推測される。
Related Reference	ACC P48635 PmId [8416893] Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. (J Bacteriol. , 1993) [7849045] ( , ) comment Blast 28th, id41%, 5e-63 Saccharopolyspora erythraea_eryK Erythromycin C-12 hydroxylase(EC 1.14.13.154) --- [PMID: 8416893](1993) Saccharopolyspora erythraea (Streptomyces erythraeus)において、P450 monooxygenase familyに属するEryKが、erythromycin生合成の最終反応の１つであるmacrolactone ringのC-12 hydroxylationに関与していることをeryK変異体を作成して調べている。 --- [PMID: 7849045](1995) 不溶性封入体としてE.coliでEryKを過剰産生し、可溶化、再折りたたみ、active holo-EryK再構築をした。動態確認で、erythromycin Bより1200-1900倍もerythromycin Dを基質として好むことが分かった。

Sequence

>putative cytochrome P450 [putative cytochrome P450]
MSVSQSERTLSNFLAYLDGLRARGGVHFDEKIKAWHVLGYHDTQQVQTDPVTFSSEVAPL
APKQEDFELSNKGNFVRADDPEHRRLRGLVSKAFTPKMIADLEPRIAELATQLLDAADAR
GGRWDFIKELGHPLPFLVMTELLGIPEDDRAFIRNLSDRFVEVQSYDAEESLDGQKENAA
NNVAPLLHELNQYLLDILRARRENPSDDLAGRLMTVEADGARLDEEEALGFLNLLVAGHH
TTTATLGNTVLALGENPHGWDQLRADPALIPAAIEESVRFRPPFPRSARQTTKDTELGGR
HIPAGAVVLVWLTAANRDDRVFADPNRFDILRKPNPHLSFGKGIHHCLGAPLARLETRTV
IRLMLERYRDIRVRDDAPVTLRNPWQMIGVSKLPIEVRPY

>putative cytochrome P450 [putative cytochrome P450]
ATGAGTGTGAGCCAGTCCGAACGGACACTGTCCAACTTCCTCGCCTATCTCGACGGGCTG
CGGGCGCGTGGAGGCGTGCATTTCGACGAGAAGATCAAGGCGTGGCACGTGCTCGGTTAC
CACGACACCCAGCAGGTGCAGACCGACCCGGTGACGTTCTCCTCCGAGGTCGCCCCGCTG
GCGCCGAAACAAGAAGACTTCGAGCTGTCCAACAAGGGCAACTTCGTCCGGGCCGACGAC
CCTGAACACCGCCGGCTGCGCGGCCTGGTCAGCAAGGCGTTCACTCCGAAAATGATCGCC
GACCTCGAGCCGCGCATCGCCGAACTGGCCACGCAGCTGCTCGATGCCGCCGACGCCCGC
GGCGGCCGGTGGGACTTCATCAAAGAACTGGGCCACCCGCTGCCGTTCCTGGTCATGACC
GAACTGCTCGGCATCCCCGAAGACGACCGCGCCTTCATCCGGAACCTGTCCGACCGGTTC
GTCGAGGTGCAGAGCTACGACGCGGAGGAGTCGCTGGACGGCCAGAAGGAAAACGCGGCG
AACAACGTCGCGCCGCTGTTGCACGAGCTGAACCAGTACCTCCTGGACATCCTCCGCGCC
CGCCGTGAAAACCCCAGCGACGACCTGGCCGGAAGGCTCATGACGGTCGAGGCCGACGGG
GCGCGGCTGGACGAGGAGGAGGCCCTCGGTTTCCTCAACCTGCTGGTCGCCGGCCACCAC
ACCACCACCGCCACCCTGGGCAACACGGTTCTCGCGCTCGGCGAGAACCCCCACGGGTGG
GACCAGCTGCGGGCCGACCCGGCGCTGATCCCGGCCGCGATCGAGGAGTCGGTGCGCTTC
CGTCCGCCCTTCCCGCGTTCGGCCCGGCAGACGACGAAGGACACCGAACTCGGCGGCCGG
CACATCCCGGCCGGCGCGGTCGTCCTGGTGTGGCTGACCGCCGCGAACCGCGACGACCGC
GTCTTCGCCGACCCGAACCGGTTCGACATCCTCCGCAAGCCCAATCCGCACCTGTCGTTC
GGCAAGGGCATACACCACTGCCTGGGCGCACCACTGGCACGGCTGGAGACACGGACCGTG
ATACGCCTCATGCTGGAGCGCTACCGTGACATCCGCGTGCGCGACGACGCCCCCGTCACC
CTGCGCAACCCCTGGCAGATGATCGGCGTCAGCAAGCTGCCCATCGAGGTCCGCCCCTAC
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) PF00067 p450 G3DSA:1.10.630.10 Cyt_P450 SSF48264 Cytochrome_P450 PR00385 P450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Rubra_00470 Rubra_00490