Soraph_00020 : CDS information

Location

Organism	Sorangium cellulosum
Strain	So ce26
Entry name	Soraphen
Contig	U24241
Start / Stop / Direction	1,424 / 6,127 / + [in whole cluster] 1,424 / 6,127 / + [in contig]
Location	1424..6127 [in whole cluster] 1424..6127 [in contig]
Type	CDS
Length	4,704 bp (1,567 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	unknown
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	hypothetical protein; Orf2
Reference	ACC Q9ADM1 PmId [12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002) [15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004) comment [PMID: 12039053](2002) Soraphen A biosynthetic gene clusterの報告。 Orf2:(1567aa): None -- [PMID: 15289572](2004) clusterのupstream borderを検討。 sorD, E and orf3は遺伝子破壊の対象とできなかったので、orf2, sorCで調査。 orf2破壊株してもsoraphen A産生可能。 sorC破壊株はsoraphen A非産生。よってupstream borderはorf2とsorCの間にある。

Sequence

>hypothetical protein [unknown]
MRSFPKRFNFSPDTWAGPRAATFHRREDAFQQLVGDALREEFGAGVRVMPTEGRDGGIDV
LLENDARLPFVSRDLAGPVIVECKDNDDARGRAFENVRAAWKKVEEKLAKQSAAGWPGTY
RPWATARTYLYLTSAALPNQDARNRLQHDVEQFFQRLRRDGRTTIEHPVVADWTDLCSLL
DRNPRLADRWLGLGRLSLRSQAEQLASLGGVSRYLVDLPFIAPGRESQTHPDRLLAHVLE
RAGEDRGVLLVGAGGVGKTRTLLEVGQRADAQGWRVLHVLSGEPPLSSSDLIQEVVDGRR
DTLVICDYFDQRALDVVSIRQRLLPAARQRGFKVAILASARTPRDARRSRNPDFFAVVRL
ALETEQAQRLAGTMEQQIAPQAVRMLGEEKVRKLTGDQPMIALLVLKELERLAATRKLTR
AQDTTVRPGELRHWLERRLAEDELVADAGSSRWDEEATSHVLGAAAALAFCPARPKHVYA
VITCVIAEENKAKAADQAERIVQNLIELGWLRNAEGKLTVAHDVVTDQLLEQALRTTSDA
VRRHALRRLLRPCAKSPAVLARFATALRRVIAAGGPFMRELERRAQAWLRKASPALGRML
AGASASKSSAALWAMLRGSPWKQTTTACWDQIVAPWLVRHGASSDAWPPLYALCIRKNLP
EPYAARALEAAIAWIEQHHEDEHAASILLMLLDRPDLGSAIASRLFELAWPWYERHRQRP
SAALVLGGLLERTEAPPALIERLGPETLRWWIEHPEVEEAGLPLGMLLRREDTSSALSQR
VEGASWEWLERHRDAQTAGYVLHGLLLTCDEESARWHRAVDAALRWLKIHADTAEGGLVL
GSLLLRGESIPEQLPAVLDAAWAWFKRRRRSFRVSFILPGLLTTSALDPDRFVQAAKIAL
GWVAAYDRYPQARILVHLVSRRGDLDPERAGAIDREACSILQKGPDMSLSGLLLGMLLER
GTLPDDVAARVAELALGWLEKNVGGELHWLVLATSIACPHVDVTDARRLAADAIAQAESG
AVRNVDGAVLGSIIHRIGIETSLLQRASAQMAQWFDGHASDLAAIQAYRLLLGRPDLDPG
LMARLVEGAQLWLEQHGDMLGAVAVIAPLLAREDLTPEIRARAETTAITWLEQHNPRFEA
VDIINVLLATSRTINKPGISIVAAAREWLQWHSRRPLASSLIANLIPPSAEPASPEDIQH
ALSWLEAHGDKPDAFDLLLRLSARRDLSPRSASVVVSTGLSWLSAHPHAELAAPLIYTLL
VRPEIDASVVVQLVSRATDWLDTHELSEASGYALAAVMFAAQRRPELAHSVDGRGPLGAV
EWLRDHASTHIAGPVIALLYSRKNLTRTTLAAAATSVKQWLGEHAHREESLWPLAALLQH
RNLPRGLARRVVQKAVEWIDHHTHVGDAYAILHELFGRDLRDEDFRTVFLAALRWLGEHV
DTPEGQQLLRLVFSQASPEESGATEGQDFVESLVDTLASSVEGEGFEDACRALPALLVEA
ARSRAPALERRARVLVARALRDRRMHRELRRRMAGACQRLLEADAWPDRARALQLLSELG
LAPALED

>hypothetical protein [unknown]
ATGCGCTCCTTCCCCAAGCGGTTCAACTTCAGCCCCGACACCTGGGCCGGTCCGCGCGCG
GCCACGTTCCACCGGCGTGAGGACGCATTCCAGCAGCTCGTGGGCGACGCGCTCCGCGAG
GAGTTCGGGGCCGGCGTCCGCGTCATGCCCACCGAGGGACGGGACGGCGGTATCGACGTG
CTCCTAGAGAACGACGCGCGCCTGCCCTTCGTTTCGCGAGACCTGGCCGGGCCGGTGATC
GTCGAGTGCAAGGACAACGACGACGCCCGTGGACGCGCCTTCGAGAACGTGCGCGCGGCA
TGGAAGAAGGTGGAGGAGAAGCTCGCGAAGCAGTCCGCGGCGGGGTGGCCCGGCACGTAC
CGCCCCTGGGCGACCGCGCGCACGTACCTCTACCTCACGAGCGCGGCGCTACCGAACCAG
GACGCCCGCAACCGGCTCCAGCATGACGTCGAGCAGTTCTTCCAGCGCTTGCGCCGGGAT
GGCAGGACCACGATCGAGCACCCGGTCGTTGCCGACTGGACCGACCTGTGCTCGCTCCTC
GACCGGAACCCGCGCCTCGCCGATCGCTGGCTCGGCCTCGGCCGGCTGTCGCTGCGCTCG
CAAGCGGAGCAGCTCGCCTCCCTCGGGGGCGTGAGCCGTTACCTCGTGGATTTGCCGTTC
ATCGCTCCAGGCCGGGAGAGCCAGACCCATCCTGACCGCCTGCTGGCCCACGTTCTCGAG
CGCGCCGGCGAGGATCGCGGCGTGCTGCTCGTCGGCGCGGGCGGCGTCGGCAAGACCCGG
ACGCTCCTCGAGGTGGGCCAGCGCGCCGATGCCCAAGGGTGGAGAGTGCTCCACGTCCTC
TCGGGCGAGCCGCCGCTGAGCTCCTCGGACCTGATCCAGGAGGTCGTGGACGGGCGACGG
GACACCCTCGTCATCTGCGACTACTTCGATCAGCGCGCCCTTGATGTCGTATCGATTCGA
CAGCGTCTCTTGCCCGCGGCGCGCCAGCGCGGGTTCAAGGTGGCGATCCTCGCCTCTGCG
CGTACGCCGCGCGACGCGCGGCGCAGCAGGAACCCGGACTTTTTCGCCGTTGTGCGTCTC
GCCCTTGAGACGGAGCAGGCGCAGCGCCTCGCAGGCACCATGGAGCAGCAGATCGCCCCT
CAGGCTGTCCGCATGCTGGGGGAGGAGAAGGTTCGCAAGCTCACCGGCGATCAGCCGATG
ATCGCGCTGCTCGTGCTCAAGGAGCTCGAGCGGCTCGCGGCCACGAGGAAGCTCACCCGT
GCGCAGGACACCACGGTCCGGCCCGGCGAGCTCCGCCACTGGCTCGAGCGCCGCCTCGCG
GAGGACGAGCTCGTCGCCGACGCCGGTTCCTCGCGCTGGGACGAGGAGGCGACGTCCCAC
GTCCTCGGCGCCGCAGCAGCGCTGGCGTTCTGCCCAGCGCGCCCGAAGCACGTCTACGCG
GTGATCACCTGCGTCATCGCCGAGGAGAACAAGGCCAAGGCGGCGGACCAGGCGGAGCGA
ATCGTCCAGAACCTCATCGAGCTCGGCTGGCTGCGGAACGCGGAAGGCAAGCTGACGGTC
GCGCACGACGTGGTCACCGATCAGCTGCTCGAACAGGCGCTGCGTACCACGAGCGACGCC
GTGCGCAGGCATGCTCTTCGGCGGTTGCTCCGGCCGTGCGCGAAGTCGCCTGCCGTGCTT
GCGCGGTTCGCGACCGCGCTCCGGCGCGTCATCGCCGCGGGGGGCCCGTTTATGCGGGAG
CTGGAGCGGCGCGCGCAGGCGTGGCTCAGGAAGGCGTCCCCTGCGCTGGGCAGGATGCTC
GCTGGCGCGTCGGCGTCGAAGTCCTCCGCGGCGCTGTGGGCCATGCTCCGTGGATCGCCA
TGGAAGCAGACGACGACCGCGTGCTGGGATCAGATCGTCGCCCCGTGGCTCGTCCGCCAT
GGCGCGTCGAGCGACGCGTGGCCCCCGCTCTACGCCCTCTGCATTCGGAAGAACCTGCCC
GAGCCGTACGCCGCGCGCGCGCTGGAGGCCGCCATCGCCTGGATCGAGCAGCACCACGAG
GACGAGCACGCCGCCAGCATCCTCCTCATGCTCCTCGACCGGCCGGACCTCGGGTCCGCG
ATTGCCTCTCGGCTGTTCGAACTGGCCTGGCCCTGGTATGAGCGGCACCGGCAGAGGCCC
TCGGCAGCGCTCGTGCTCGGCGGGCTCCTCGAGCGCACGGAGGCGCCGCCTGCACTCATC
GAGCGCCTTGGACCGGAGACGCTTCGCTGGTGGATCGAGCACCCGGAGGTCGAAGAGGCC
GGGCTCCCGCTCGGCATGCTGCTCCGGCGAGAGGACACCAGCTCTGCGCTGAGCCAGCGG
GTCGAGGGCGCGTCCTGGGAGTGGCTCGAGCGGCATCGCGACGCGCAGACCGCCGGCTAC
GTCCTCCACGGGCTCTTGCTGACCTGCGACGAAGAATCCGCCCGCTGGCACCGGGCCGTC
GATGCCGCGCTCCGCTGGCTCAAAATCCACGCTGACACCGCAGAGGGCGGGCTCGTGCTC
GGCAGCCTTCTCCTGAGAGGAGAGTCGATCCCGGAGCAGCTTCCCGCCGTGCTCGACGCC
GCGTGGGCATGGTTCAAGCGGCGCAGACGTTCGTTCCGCGTCTCGTTCATCCTGCCCGGG
CTGCTCACGACGAGCGCGCTCGATCCGGACAGGTTCGTTCAGGCGGCGAAGATCGCGCTC
GGCTGGGTCGCGGCCTACGACAGGTATCCACAGGCAAGAATCCTCGTCCACCTGGTCTCC
CGACGCGGCGATCTCGACCCGGAGCGCGCCGGTGCGATCGACCGTGAAGCGTGCAGTATC
CTGCAGAAGGGTCCGGACATGTCACTCTCCGGCCTCCTCCTCGGTATGCTGCTGGAACGC
GGCACGCTGCCCGACGATGTCGCAGCCAGGGTGGCCGAGCTGGCGTTGGGCTGGCTCGAG
AAGAACGTCGGCGGCGAGCTGCACTGGCTGGTGCTGGCAACCAGCATCGCCTGCCCACAC
GTCGACGTCACGGACGCGCGCCGGCTCGCGGCCGACGCCATCGCGCAGGCGGAGTCCGGA
GCTGTACGGAACGTGGACGGCGCGGTGCTGGGGAGCATCATCCACCGCATCGGCATCGAG
ACATCGCTCCTTCAGCGCGCGTCGGCGCAGATGGCGCAGTGGTTCGACGGTCACGCGTCC
GACCTCGCCGCAATCCAGGCCTATCGCCTGCTGCTCGGGCGCCCGGATCTCGATCCGGGC
CTCATGGCGCGACTCGTCGAGGGCGCGCAGCTCTGGCTGGAACAGCACGGCGATATGTTG
GGGGCTGTCGCCGTCATCGCGCCGCTCCTCGCGCGCGAGGACCTGACGCCGGAGATCCGG
GCTCGCGCCGAGACGACGGCGATAACATGGCTCGAGCAGCACAACCCGCGGTTCGAGGCA
GTCGACATCATCAACGTCCTGCTCGCGACATCCCGCACAATAAACAAACCTGGCATTTCG
ATCGTCGCAGCCGCGAGAGAATGGCTGCAATGGCATTCAAGGCGTCCGCTGGCATCGTCG
CTGATAGCGAATCTCATTCCGCCGTCAGCAGAACCGGCCAGCCCAGAGGATATCCAGCAC
GCCCTCTCCTGGCTGGAAGCACACGGCGACAAGCCAGATGCTTTCGATCTTCTGCTGAGG
TTGTCCGCGCGCCGCGACCTGAGCCCGCGCTCCGCGAGCGTCGTGGTCTCCACAGGTCTG
TCCTGGCTCTCCGCTCACCCACACGCGGAGCTGGCCGCACCCCTGATTTACACGCTGTTG
GTCAGACCAGAGATAGACGCATCGGTCGTCGTCCAGCTGGTATCCAGAGCAACCGACTGG
CTCGACACGCACGAGCTCTCGGAGGCCTCCGGATACGCGCTGGCAGCCGTGATGTTCGCC
GCTCAAAGGCGACCAGAACTCGCACACAGCGTCGACGGGCGCGGACCGCTCGGGGCGGTA
GAATGGCTGCGAGACCATGCGAGCACGCACATCGCGGGTCCGGTCATAGCGCTCCTCTAC
AGCAGAAAAAACCTCACAAGGACAACGCTCGCCGCGGCAGCGACGAGCGTGAAACAATGG
CTCGGCGAGCACGCGCACCGGGAAGAGTCATTGTGGCCCCTGGCGGCGCTTCTGCAACAC
AGGAACCTGCCACGCGGACTCGCGAGAAGGGTGGTCCAGAAAGCGGTCGAGTGGATAGAC
CATCACACGCACGTCGGAGACGCCTACGCGATACTACACGAGTTGTTTGGCCGCGATCTG
CGTGACGAAGACTTCCGCACTGTCTTCCTGGCGGCGCTGCGATGGCTCGGGGAACACGTC
GACACTCCGGAGGGCCAGCAACTGTTGCGCCTCGTCTTCAGCCAAGCTTCACCCGAGGAG
AGCGGGGCAACTGAAGGCCAGGACTTTGTCGAGTCGCTCGTGGATACTTTGGCCTCGTCC
GTGGAGGGCGAGGGCTTCGAGGATGCCTGCCGGGCCCTGCCGGCGCTACTCGTGGAGGCT
GCGCGGAGCAGAGCACCAGCGCTGGAGCGAAGAGCTCGGGTCCTGGTTGCGCGAGCGCTC
CGCGACCGGCGGATGCACCGAGAGCTGCGGCGCCGCATGGCCGGGGCGTGTCAACGGCTG
CTCGAGGCGGACGCGTGGCCGGACCGAGCGCGCGCCCTCCAGTTGCTGAGCGAGCTAGGT
TTGGCGCCAGCGCTGGAGGACTAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Soraph_00010 Soraph_00025