Soraph_00025 : CDS information

Location

Organism	Sorangium cellulosum
Strain	So ce26
Entry name	Soraphen
Contig	U24241
Start / Stop / Direction	6,813 / 8,849 / + [in whole cluster] 6,813 / 8,849 / + [in contig]
Location	6813..8849 [in whole cluster] 6813..8849 [in contig]
Type	CDS
Length	2,037 bp (678 aa)

Annotation

Category	5.2 function unknown
Product	Rhs element Vgr family protein
Product (GenBank)
Gene
Gene (GenBank)
EC number
Keyword
Note	Orf3
Note (GenBank)
Reference	PmId [12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002) comment [PMID: 12039053](2002) Soraphen A biosynthetic gene clusterの報告。 Orf3(677aa): 機能推定なし N末側はP.aeruginosaのhypo、V.choleraeの機能不明VgrG proteinに似ている。 sorE geneとオーバーラップし、逆向きに転写される。
Related Reference	ACC A8YQR5 PmId [17986187] Dissection of a type VI secretion system in Edwardsiella tarda. (Mol Microbiol. , 2007) comment 42nd(A8YQR5) 29%, 6e-35, C末側はalignment悪い Edwardsiella tarda_evpI EvpI Edwardsiella tarda virulent protein (EVP) gene clusterの報告。 type VI secretion system (T6SS)と2つの追加分泌タンパク (EvpI, a homologue of VgrG, and EvpP) が見つかり、entire EVP clusterとなった。 16 EVP genesの変異誘発から、EvpPの分泌がEvpC (a homologue of Hcp) and EvpIを含む13 EVP proteinsに依存的で、EvpD and EvpJには依存しないことがわかった。分泌タンパクはEvpC, EvpI and EvpPの3つで、EvpC and EvpI の分泌は相互に依存し、それらはEvpPの分泌のために必要である。
Family/Domain	FD IPR017847 comment [IPR017847] Rhs element Vgr protein, subgroup (Family) (IPR017847 Type VI secretion system, Rhs element Vgr family)

Sequence

>Rhs element Vgr family protein
MMDGTLSHVLLAGSGLPGDVRVQSYEAHEAVSLPYAVHVELATSDPSFRIDECLQRRMVL
EVGDGQGGVRYYDGLPDRVGFLAYRGGEFFFRMRLRPALAALEHREGSRIFQDKTPVDVV
KTILAEAGVDTDVEWRLRQTYAPREFLCQYRETEMNFVHRILEDEGIFYFFLHSPDGHKL
VFCDDPAAFAQEEGTPPVVLAPRQGGVAGTQPLLEFRREKTLRATEVVLRDYDFEKPELP
PTATTPAPGPWSIRHFQYPGGFTSSAEGSRRSRGRISSLRGDVDVCRGRSRAAGLRCGAP
MTVEGANEPFLNGEFVAVELRSRGRSGEDACENEFAAVPKGAPFAAPLRAKKPRIRGIQT
AVVTGPSNEPQSIHVDAKYLAIAVAESAPGSRRSRRRPASMHSISEACRSVNMSLIIRRT
SWCSFSGAPNCSRRLVYATQASTHARRMPTQPHATVYRPRSSATEATCVMPKPRRPTTWS
ASTRHPSRCTLAVIDARMPSRSRIGSVRRPGRPRSTRTAVGPSSVRPKTRKRSAISPYVI
HLFSPSITRSVPWAAIRVVTLLASLPTPGSDSASAPIRRPDASSGSHRSRCARVPHRRIA
SATIVWTVSRLRTDEQPRASSAARSPSVTASSPAPPNSLGMEMPRYPRSARSAMSSGGVR
WARWNASANGATCSSTKP

>Rhs element Vgr family protein
ATGATGGATGGCACGCTCAGCCACGTGCTCCTTGCGGGCAGCGGTCTGCCCGGCGACGTA
CGGGTCCAGTCCTACGAGGCGCACGAGGCGGTGTCGCTGCCGTACGCGGTCCACGTTGAG
CTCGCGACCAGCGACCCGTCGTTCCGCATCGACGAGTGCCTCCAGCGCCGGATGGTGCTC
GAGGTCGGGGACGGACAGGGGGGCGTCCGATACTACGACGGCCTCCCGGACCGCGTCGGC
TTCCTCGCGTACCGAGGTGGCGAGTTCTTCTTTCGCATGCGACTCCGCCCCGCGTTGGCC
GCGCTCGAGCACCGCGAGGGATCGCGCATCTTCCAGGACAAGACCCCGGTCGACGTCGTC
AAGACCATCCTGGCCGAGGCCGGCGTCGACACGGACGTGGAATGGCGGCTCCGGCAGACC
TACGCCCCGCGCGAGTTTCTCTGCCAGTACCGCGAGACGGAGATGAACTTCGTCCACCGC
ATCCTCGAGGACGAGGGCATCTTCTACTTCTTTCTCCACTCGCCCGACGGCCACAAGCTT
GTCTTCTGCGACGACCCCGCCGCCTTCGCGCAGGAAGAGGGCACCCCGCCCGTCGTGCTC
GCGCCGCGCCAGGGCGGGGTCGCGGGGACACAGCCCCTTCTTGAATTCCGCCGCGAGAAG
ACGCTGCGCGCTACCGAGGTGGTGCTGCGCGACTACGACTTCGAGAAGCCCGAGCTACCC
CCTACGGCGACGACCCCCGCACCAGGTCCGTGGTCCATCCGCCACTTCCAATACCCGGGC
GGCTTCACCTCGAGCGCCGAGGGATCGCGCCGGTCGCGGGGCCGTATCAGCTCGCTGCGC
GGTGACGTGGACGTCTGCCGCGGGAGGAGCCGCGCGGCAGGCCTCCGCTGCGGCGCGCCG
ATGACGGTCGAGGGGGCCAACGAGCCGTTCCTGAATGGCGAGTTCGTCGCGGTAGAGCTG
CGATCGCGAGGTCGCAGCGGCGAGGATGCGTGCGAGAACGAGTTCGCCGCCGTCCCCAAG
GGCGCGCCCTTCGCCGCGCCCCTGCGCGCGAAGAAGCCCAGGATCCGCGGCATCCAGACG
GCCGTCGTGACGGGCCCCTCGAACGAGCCCCAGTCGATCCACGTCGACGCGAAGTACTTG
GCCATCGCCGTCGCCGAGAGCGCGCCGGGATCTCGGCGGTCACGCAGGCGGCCGGCCTCC
ATGCACAGCATCTCCGAGGCGTGCAGGTCCGTGAACATGTCGCTGATCATCCGCCGGACG
AGCTGGTGCTCCTTCAGCGGCGCGCCGAATTGCTCGCGGCGGCTCGTGTACGCGACGCAG
GCCTCGACGCACGCCCGGAGGATGCCCACGCAGCCCCACGCGACGGTGTACCGGCCCAGA
TCGAGCGCGACGGAGGCGACCTGCGTGATGCCCAAACCTCGCCGCCCCACCACGTGGTCG
GCCTCCACGCGGCATCCATCGAGATGCACGCTCGCGGTCATCGACGCGCGGATGCCCAGC
AGATCGCGGATCGGCTCGGTCCGGAGGCCCGGCCGACCGCGCTCGACGAGGACAGCCGTG
GGCCCTTCCTCGGTGCGCCCGAAGACGAGGAAGAGGTCGGCGATCTCGCCGTACGTGATC
CACCTCTTCTCGCCGTCGATCACGAGATCGGTCCCCTGGGCGGCGATCCGCGTCGTGACG
CTCTTGGCGTCGCTGCCGACGCCCGGCTCGGACAGCGCGAGCGCCCCGATCCGGCGACCC
GACGCGAGCTCCGGCAGCCACCGTTCTCGCTGCGCGCGGGTGCCCCACCGGAGGATCGCC
AGCGCCACCATCGTGTGGACGGTGAGCAGGCTGCGCACCGACGAGCAGCCGCGGGCGAGC
TCCGCCGCGAGGAGCCCGAGCGTCACGGCGTCCTCGCCGGCACCCCCGAACTCTTTGGGG
ATGGAGATGCCGAGGTACCCGCGCTCGGCGAGGAGCGCGATGAGCTCCGGCGGCGTCCGC
TGGGCCCGGTGGAACGCGTCGGCGAACGGCGCGACGTGCTCGTCGACGAAACCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR006533 Rhs element Vgr protein (Family) TIGR01646 vgr_GE: Rhs element Vgr protein IPR017847 Type VI secretion system, Rhs element Vgr family (Family) TIGR03361 VI_Rhs_Vgr: type VI secretion system Vgr family pr
SignalP	No significant hit
TMHMM	No significant hit

Soraph_00020 Soraph_00030