Soraph_00035 Soraph_00035   Soraph_00050 Soraph_00050

Soraph_00040 : CDS information

close this sectionLocation

Organism
StrainSo ce26
Entry nameSoraphen
Contig
Start / Stop / Direction10,080 / 9,199 / - [in whole cluster]
10,080 / 9,199 / - [in contig]
Locationcomplement(9199..10080) [in whole cluster]
complement(9199..10080) [in contig]
TypeCDS
Length882 bp (293 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.1 modification addition of extender units
Productputative oxidoreductase
Product (GenBank)beta-hydroxybutyryl-CoA dehydrogenase
Gene
Gene (GenBank)sorD
EC number
Keyword
  • methoxymalonyl-ACP
Note
Note (GenBank)
Reference
ACC
PmId
[12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002)
[15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004)
comment
[PMID: 12039053](2002)
Soraphen A biosynthetic gene clusterの報告。
SorD(295aa): beta-Hydroxybutyryl-CoA dehydrogenase

Module 3,7で取り込まれるMethoxymalonyl-CoAを合成する。
本ORFはCH2OHをCHOにする

---
[PMID: 15289572](2004)
sorC破壊株はsoraphen A非産生。

S. lividansでのsor genes異種性発現。
SorABRCDFE株 + cinnamate
SorABRCDFE株 + badA(benzoate-CoA ligase) + benzoate or cinnamate
の組み合わせでSoraphen A産生。
SorABRCDFE株で発現されているのは、the sorA, and the sorB4M, sorRCDFE operons.
Related Reference
ACC
Q7BQ74
PmId
[16983083] Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. (Proc Natl Acad Sci U S A. , 2006)
comment
22nd(Q7BQ74) 51%, 9e-78
Bacillus cereus_zmaG
ZmaG

ZmaGは、珍しいPKS伸長units, hydroxymalonyl-ACP and aminomalonyl-ACPの生合成に共通して関連する。

ZmaE or ZmaIと共に働く。
ZmaI: eryl-ZmaH(ACP)→aminomalonyl-ZmaH(ACP)
ZmaE: glyceryl-ZmaD(ACP)→hydroxymalonyl-ZmaD(ACP)
ACC
P52041
PmId
[8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996)
comment
149th, id45%, 4e-63
Clostridium acetobutylicum_hbd
3-hydroxybutyryl-CoA dehydrogenase

beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase (BHBD)の遺伝子のクローニングを行い、E. coliで過剰発現させたところBHBDの細胞外への分泌を確認。
また、C. acetobutylicumで発現させ、BHBD activityの上昇を確認している。

close this sectionSequence

selected fasta
>putative oxidoreductase [beta-hydroxybutyryl-CoA dehydrogenase]
MDRSAELQYPVVGVVGAGVMGVGVAQSLAQTGHDVVLVDVSEAALARARMGIRNGLRAVT
LFGSAEDKKRAGDPKAVLERVAFTTDYGRLAGADFVVENVTEKWDIKREVYARLEGVCRP
EIIFAADTSAISITRIGSVTKRPSQVVGMHFMNPVPLKPMVEVIRGFHTSPETLGAAKRF
LAEMGKTCVVVEDAPGFVSNRVLMLTINEAIFLVQDQVASAADVDRIFKGCFEHKMGPLE
TADLIGLDTILYSIEVLYESFNDDKYRPCPLLKKMVDAGLHGRKSGRGFYTYE
selected fasta
>putative oxidoreductase [beta-hydroxybutyryl-CoA dehydrogenase]
ATGGATCGCTCTGCTGAATTGCAGTACCCGGTGGTCGGAGTCGTCGGCGCGGGCGTCATG
GGCGTCGGCGTCGCCCAGAGCCTCGCGCAAACCGGCCACGACGTGGTCTTGGTCGACGTC
TCGGAGGCGGCCCTCGCGCGGGCGCGAATGGGGATCCGGAACGGCCTGCGCGCCGTCACG
CTCTTCGGCAGCGCCGAGGACAAGAAGCGCGCCGGGGATCCGAAGGCGGTGCTCGAGCGC
GTGGCGTTCACGACCGATTATGGGCGCCTCGCCGGCGCCGATTTCGTGGTCGAGAACGTG
ACGGAGAAATGGGATATCAAGCGCGAGGTGTACGCCCGCCTTGAGGGCGTCTGCCGGCCG
GAGATCATCTTCGCGGCGGACACCTCTGCCATCTCGATCACGCGGATAGGCTCGGTCACG
AAGCGTCCTTCGCAGGTGGTCGGCATGCACTTCATGAACCCGGTCCCGCTCAAGCCGATG
GTCGAGGTGATCCGCGGCTTCCACACGTCCCCCGAGACGCTGGGCGCCGCCAAGCGCTTC
CTCGCCGAGATGGGCAAGACGTGCGTCGTCGTCGAGGACGCGCCGGGGTTCGTGTCGAAC
CGCGTGTTGATGCTGACCATCAACGAGGCGATCTTCCTGGTCCAGGATCAGGTCGCGAGC
GCGGCCGACGTGGATCGCATCTTCAAGGGGTGCTTCGAGCACAAGATGGGGCCGCTCGAG
ACGGCCGACCTCATCGGGCTCGATACGATCCTCTATTCCATCGAGGTCCTCTACGAGAGC
TTCAACGACGACAAGTACCGCCCGTGCCCGCTGCTCAAGAAGATGGTGGACGCGGGGCTC
CACGGCCGGAAGTCGGGCCGCGGGTTTTACACCTACGAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal (Domain)
[196-292] 5.20000000000001e-32 PF00725
PF00725   3HCDH
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding (Domain)
[12-193] 1.29999999999998e-61 PF02737
PF02737   3HCDH_N
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like (Domain)
[196-293] 3.69999438558106e-31 SSF48179
SSF48179   6DGDH_C_like
IPR013328 Dehydrogenase, multihelical (Domain)
[198-293] 2.80000000000001e-30 G3DSA:1.10.1040.10
G3DSA:1.10.1040.10   Opine_DH
IPR016040 NAD(P)-binding domain (Domain)
[12-197] 2.19999999999997e-53 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR022694 3-hydroxyacyl-CoA dehydrogenase (Family)
[1-293] 8.89993011983204e-65 PIRSF000105
PIRSF000105   HCDH
SignalP
[1-25] 0.232 Signal
Eukaryota   
TMHMM No significant hit
Soraph_00035 Soraph_00035   Soraph_00050 Soraph_00050
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