Soraph_00080 Soraph_00080   Soraph_00100 Soraph_00100

Soraph_00090 : CDS information

close this sectionLocation

Organism
StrainSo ce26
Entry nameSoraphen
Contig
Start / Stop / Direction59,795 / 60,226 / + [in whole cluster]
59,795 / 60,226 / + [in contig]
Location59795..60226 [in whole cluster]
59795..60226 [in contig]
TypeCDS
Length432 bp (143 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.4 hypothetical protein
Producthypothetical protein
Product (GenBank)unknown
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • similar to hypothetical protein MmyY from Streptomyces coelicolor; Orf4
Reference
ACC
PmId
[12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002)
[15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004)
comment
[PMID: 12039053](2002)
Soraphen A biosynthetic gene clusterの報告。
Orf4(167aa): Hypothetical protein

aglycon3位と7位での架橋構造を形成するためにC-3 keto基をもたらすoxidaseとして機能するか、C-9 and C-10間の二重結合をもたらすoxidoreductaseであると提唱されている。
ORF4も同じ役割をする酵素の候補であるとされているが、理由はsorB and sorMの間というlocationである。

---
[PMID: 15289572](2004)
S. lividansでのsor genes異種性発現。

SorABRCDFE株 + cinnamate
SorABRCDFE株 + badA(benzoate-CoA ligase) + benzoate or cinnamate
の組み合わせでSoraphen A産生。

SorABRCDFE株で発現されているのは、the sorA, and the sorB4M, sorRCDFE operons.

close this sectionSequence

selected fasta
>hypothetical protein [unknown]
MDPKRIQSVIERIEIIDTLTTFTRAADMREWDTCRAILGETIINDHGTPETLSRDALIER
WRVQSPTLDLIHHVTTDHLVVIDGDLAKVHTQFIITVRATGAPSGDLCTRGGSCDYDLKR
TDEGWKLTGFKSVIQWSMGSVNI
selected fasta
>hypothetical protein [unknown]
ATGGACCCGAAACGAATCCAATCCGTCATTGAACGCATCGAAATCATCGACACGCTCACC
ACCTTCACCCGCGCAGCAGACATGCGTGAATGGGATACGTGTCGGGCCATCCTCGGAGAG
ACGATCATCAACGATCACGGCACGCCCGAGACTCTCTCTCGCGACGCGCTCATCGAGCGG
TGGCGTGTACAGTCGCCCACGCTCGACCTGATCCATCACGTGACCACGGATCATCTCGTC
GTGATCGATGGCGACCTCGCGAAGGTCCACACCCAGTTCATTATCACGGTGCGCGCGACG
GGCGCGCCGAGCGGCGATCTGTGCACGCGCGGCGGCTCCTGCGATTACGATCTGAAGCGG
ACCGATGAAGGCTGGAAGCTCACGGGCTTCAAGTCGGTCATCCAGTGGAGCATGGGGAGC
GTGAACATCTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP
[1-25] 0.046 Signal
Bacteria, Gram-negative   
[1-25] 0.109 Signal
Eukaryota   
TMHMM No significant hit
Soraph_00080 Soraph_00080   Soraph_00100 Soraph_00100
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