Soraph_00100 : CDS information

Location

Organism	Sorangium cellulosum
Strain	So ce26
Entry name	Soraphen
Contig	U24241
Start / Stop / Direction	60,255 / 61,295 / + [in whole cluster] 60,255 / 61,295 / + [in contig]
Location	60255..61295 [in whole cluster] 60255..61295 [in contig]
Type	CDS
Length	1,041 bp (346 aa)

Annotation

Category	3.2 modification methylation
Product	putative O-methyltransferase
Product (GenBank)	O-methyltransferase
Gene
Gene (GenBank)	sorM
EC number	2.1.1.-
Keyword	C-11
Note
Note (GenBank)
Reference	ACC Q9ADL4 PmId [12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002) [15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004) comment [PMID: 12039053](2002) Soraphen A biosynthetic gene clusterの報告。 SorM(346aa): O-methyltransferase Aglycon形成時にはMethyl化されていない11位のOHをメチル化すると提唱。 --- [PMID: 15289572](2004) S. lividansでのsor genes異種性発現。 SorABRCDFE株 + cinnamate SorABRCDFE株 + badA(benzoate-CoA ligase) + benzoate or cinnamate の組み合わせでSoraphen A産生。 SorABRCDFE株で発現されているのは、the sorA, and the sorB4M, sorRCDFE operons.
Related Reference	ACC Q54303 NITE Rapam_00180 PmId [11372782] Deletion of rapQONML from the rapamycin gene cluster of Streptomyces hygroscopicus gives production of the 16-O-desmethyl-27-desmethoxy analog. (J Antibiot (Tokyo). , 2001) [16990929] Rapamycin biosynthesis: Elucidation of gene product function. (Org Biomol Chem. , 2006) comment 4th(Q54303) 38%, 7e-45 Streptomyces hygroscopicus_rapM [Rapam_00180]16-O-methyltransferase(EC 2.1.1.-) -- [PMID: 11372782](2001) rapQONMLのdeletion mutantはpipecolateを添加すると、rapamycinの代わりにanalog 16-O-desmethyl-27-desmethoxyrapamycinを産生した。これは、rapNによってコードされるP450 hydroxylaseがC-27に特異的で、rapQとrapM によってコードされるO-methyltransferasesがC-16 and C-27でのhydroxyl基をメチル化することを示す。 -- [PMID: 16990929](2006) rapKIJMNOQL deletion mutant S.hygroscopicus MG2-10をrapMで相補すると、C-16でのみO-methylationされたpre-rapamycin(PKSから放出された後の最初の中間体。未修飾aglycon.)が得られる。また、rapMを発現したS.lividans TK24株にpre-rapamycinを与えると、同じ化合物が得られた。 rapKIJMNOQL deletion mutant S.hygroscopicus MG2-10をrapMNOで相補すると、C-27でhydroxylateされ、C-16でmethylateされたpre-rapamycinを蓄積。よって、RapMはC-16に特異的なO-methylationを担う。

Sequence

>putative O-methyltransferase [O-methyltransferase]
MQISPGARTSPITADSKRGSNMASQQIELIKMFKRMVDAMASIQSAVDQDALAAQGGTLK
RLIEDGYNLEAAQGADRFHNWGMFHEEVYRDVLQQLPDYDKSDTDGYSEQLYVYTLKQVP
ADEDRPRKILEVGCNTGKGLNFLSRIEGRSTFVGLDLSQQAVDIANARFSRPGSLTYVQG
DAENLPFADGEFDVVINVESSHNYPNLRKSFLEVARVLRPGGFFSHVDVFSDNRYSVMQN
CKQQTSGELDWLKETDISEYVKEAIRRRLAPGSKARQQVERALPYPLGRLFSSVMMRGYG
SEFAVGQKSDDWIRNVSWLNPPGQQWLSQITSYRHTLATKARGHGS

>putative O-methyltransferase [O-methyltransferase]
ATGCAAATTTCGCCAGGGGCTCGGACATCTCCCATCACGGCGGATAGCAAACGAGGAAGC
AATATGGCATCGCAGCAGATCGAGCTGATCAAAATGTTCAAACGGATGGTGGACGCCATG
GCCAGCATCCAGTCCGCTGTCGACCAGGACGCGCTCGCCGCTCAAGGTGGCACGCTCAAG
CGCCTCATCGAGGACGGCTACAACCTCGAGGCCGCTCAAGGTGCCGATCGCTTTCACAAC
TGGGGGATGTTTCACGAGGAGGTTTATCGTGATGTTCTTCAACAGCTGCCAGATTACGAC
AAGAGCGACACAGACGGTTATTCGGAGCAGCTCTACGTCTACACGCTGAAGCAAGTTCCG
GCCGACGAAGATCGGCCACGCAAGATCCTGGAGGTCGGCTGCAACACTGGGAAGGGCCTC
AACTTCCTTTCCCGGATCGAGGGTCGCAGCACCTTCGTCGGGCTCGACCTGTCGCAGCAA
GCGGTGGACATCGCCAACGCCCGGTTCTCGCGACCGGGCTCGTTGACCTACGTTCAAGGC
GACGCGGAGAACCTTCCTTTCGCCGACGGCGAATTCGACGTCGTCATCAATGTCGAGAGC
TCGCACAACTACCCTAATCTGCGCAAATCATTCCTCGAAGTGGCGCGCGTGCTGCGACCG
GGCGGGTTCTTTTCGCATGTGGATGTCTTCTCGGACAATCGCTATTCGGTGATGCAGAAT
TGCAAGCAGCAGACCAGCGGCGAGCTCGACTGGCTCAAGGAGACCGACATCTCGGAGTAC
GTAAAAGAGGCTATCCGGCGACGACTGGCGCCCGGCAGCAAGGCAAGGCAACAGGTGGAG
CGCGCGCTGCCGTACCCGCTCGGAAGGCTTTTCAGCTCGGTCATGATGCGCGGCTACGGA
TCCGAGTTTGCCGTCGGACAGAAGTCGGACGACTGGATCAGGAATGTCTCATGGCTCAAC
CCTCCCGGCCAGCAATGGTTGTCGCAGATCACCTCGTATCGCCACACGCTCGCTACGAAG
GCGCGCGGCCACGGGTCTTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR013216 Methyltransferase type 11 (Domain) PF08241 Methyltransf_11
SignalP	No significant hit
TMHMM	No significant hit

Soraph_00090 Soraph_00110