Soraph_00110 : CDS information

Location

Organism	Sorangium cellulosum
Strain	So ce26
Entry name	Soraphen
Contig	U24241
Start / Stop / Direction	63,353 / 61,362 / - [in whole cluster] 63,353 / 61,362 / - [in contig]
Location	complement(61362..63353) [in whole cluster] complement(61362..63353) [in contig]
Type	CDS
Length	1,992 bp (663 aa)

Annotation

Category	5.1 general function
Product	putative polysaccharide lyase
Product (GenBank)	unknown
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	similar to hypothetical protein YesW from Bacillus subtilis; Orf5
Reference	ACC Q9ADL3 PmId [12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002) [15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004) comment [PMID: 12039053](2002) Soraphen A biosynthetic gene clusterの報告。 Orf5(663aa): Hypothetical protein --- [PMID: 15289572](2004) clusterの境界について検討。 C末側は配列解析(orf5はhypoで、orf6,7が一次代謝遺伝子）と、Sor. cellulosum So ce26 SJ3での遺伝子破壊でsoraphen類似体産生に影響しなかったことから、orf5-7はsor clusterでなはいと述べている。
Related Reference	ACC O31526 PmId [16682770] Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11. (Acta Crystallogr Sect F Struct Biol Cryst Commun. , 2006) [17449691] Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization. (Appl Environ Microbiol. , 2007) comment 4th(O31526) 54%, 1e-170 Bacillus subtilis_yesW Rhamnogalacturonan endolyase yesW(EC 4.2.2.23)

Sequence

>putative polysaccharide lyase [unknown]
MKRSVFLICFSLAAGLQACGSSDTGSSGADNTGGSGGDGAGTSGAGAGTPAGGGGAGTPS
GSSGSGGGGETAGPTGFAQMEDLDRGVVAVSTGDGIYVGWRLFGYDPDDIAFNVYRDGAK
VNDAPIADSTNLMDQDGSPGAVYTVRAVVGGVEQGASEGASVWRDGALSIPTEAPPAGDG
YTYSSGDGSVGDLDGDGRYEIIVKWDPSNLKDNSQAGRTGKTYLDAYSLEGERLWRIDLG
VNIRAGAHYSPFLVYDLDGDGKAEVAVKTAPGTRDGTGEPLSKGPAANDDDSRDYRNNDG
YILTGPEYLTVFSGETGAELATTDFVVGRGDPCSWGNNECYGNRVDRFVGTVAFLDDTGR
PSVVFGRGYYARTTLSAWNYRDGALTNLWTFDSSSSRDNGAYAGMGTHSISVANVDDDPQ
QEIINGGATFDNDGKGLCAVDYYGHGDALHVTDHILSRPGLEVFQPYEGGDSPAYAMRDA
RTCEVLWRGPGNGGEEGPGRGVAADVDPRNPGSEAWVNSSQLLSGADGDAIGNRPASSNF
LIWWDADLSRELLDGNSIRQADGEGSNFAAEGCTANNGSKSNPTLSADILGDWREEVIFR
CGSSIRIFTTNRVATSRIHTLMHDPQYRVAISWQNGAYNQPPHPSFHIGEGMAPVPKPDI
HVR

>putative polysaccharide lyase [unknown]
ATGAAACGATCGGTCTTCTTGATCTGCTTTTCTCTCGCCGCCGGGCTCCAGGCGTGCGGG
TCGAGTGATACGGGCTCCAGCGGAGCCGACAACACGGGCGGCTCGGGCGGGGACGGCGCC
GGCACGAGCGGCGCGGGCGCGGGCACGCCCGCCGGCGGCGGCGGCGCGGGTACACCTTCC
GGCAGCAGCGGCAGCGGCGGCGGCGGCGAGACGGCCGGGCCGACGGGGTTCGCCCAGATG
GAGGACCTCGACCGCGGCGTCGTGGCCGTCTCCACGGGCGACGGCATCTACGTGGGCTGG
CGCCTGTTCGGCTACGATCCCGACGACATCGCCTTCAACGTCTACCGCGACGGGGCCAAG
GTCAACGACGCTCCGATCGCGGACTCGACGAACCTCATGGACCAGGACGGCAGTCCGGGC
GCGGTCTACACGGTGCGCGCCGTCGTCGGCGGGGTCGAGCAAGGGGCGTCGGAGGGCGCG
TCCGTGTGGAGGGACGGGGCCCTGTCGATCCCGACCGAGGCGCCGCCCGCTGGGGACGGC
TACACGTACTCGAGCGGCGATGGCAGCGTGGGAGATCTGGACGGCGACGGGCGGTACGAG
ATCATCGTCAAGTGGGACCCGTCGAACCTCAAGGACAACTCGCAGGCTGGCCGCACCGGC
AAGACGTACCTCGACGCCTACTCGCTCGAGGGCGAGCGGCTCTGGCGCATCGACCTCGGC
GTGAACATCCGGGCCGGAGCGCACTACTCGCCGTTCCTCGTCTACGATCTCGACGGCGAC
GGGAAGGCGGAGGTGGCCGTCAAGACGGCGCCGGGGACACGCGACGGCACGGGCGAGCCC
CTCAGCAAGGGGCCCGCGGCGAACGACGACGACAGCCGGGACTACCGCAACAACGACGGC
TACATCCTGACCGGCCCGGAGTACCTCACCGTGTTCTCCGGGGAGACCGGCGCCGAGCTC
GCGACGACCGACTTCGTGGTGGGGCGCGGCGACCCGTGCAGCTGGGGCAACAACGAGTGT
TACGGCAATCGCGTCGACCGCTTCGTCGGCACGGTCGCGTTCCTCGACGACACCGGTCGT
CCGAGCGTGGTGTTCGGCCGCGGCTACTACGCGCGCACCACGCTGTCGGCGTGGAACTAC
CGCGACGGCGCGCTCACGAACCTCTGGACGTTCGACTCCAGCTCGAGCCGCGACAACGGG
GCGTACGCCGGCATGGGCACCCACTCCATCAGCGTCGCCAACGTGGATGACGATCCGCAG
CAGGAGATCATCAACGGGGGCGCCACGTTCGACAACGACGGCAAGGGCCTGTGCGCCGTG
GACTACTACGGTCACGGCGACGCGCTGCACGTCACGGATCACATCCTGTCGCGCCCCGGC
CTCGAGGTGTTCCAGCCGTACGAGGGCGGGGACTCACCCGCCTATGCCATGCGCGACGCG
CGCACGTGCGAGGTCCTCTGGCGGGGGCCGGGCAACGGCGGCGAGGAGGGCCCCGGCCGC
GGCGTGGCGGCCGACGTCGATCCGCGCAACCCGGGCAGCGAGGCGTGGGTCAATAGCAGC
CAGCTCCTGAGCGGCGCGGACGGCGACGCCATCGGGAACCGCCCCGCGTCGTCCAACTTC
CTCATCTGGTGGGACGCGGATCTGAGCCGGGAGCTGCTCGACGGCAACAGCATCCGCCAG
GCCGACGGCGAGGGAAGCAACTTCGCGGCCGAGGGCTGCACCGCGAACAACGGCTCGAAG
AGCAACCCGACCCTCAGCGCCGATATCCTCGGCGACTGGCGCGAAGAGGTGATCTTCCGC
TGCGGCAGCTCGATTCGTATCTTCACCACGAACCGCGTCGCCACGAGCCGGATCCACACC
CTGATGCACGATCCGCAGTACCGCGTGGCCATCTCGTGGCAGAACGGCGCCTACAACCAG
CCGCCTCACCCGAGCTTCCACATCGGGGAGGGGATGGCGCCGGTCCCGAAGCCGGACATC
CACGTCCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Bacteria, Gram-positive Bacteria, Gram-negative Eukaryota
TMHMM	No significant hit

Soraph_00100 Soraph_00120