Soraph_00110 Soraph_00110   Soraph_00130 Soraph_00130

Soraph_00120 : CDS information

close this sectionLocation

Organism
StrainSo ce26
Entry nameSoraphen
Contig
Start / Stop / Direction63,970 / 65,478 / + [in whole cluster]
63,970 / 65,478 / + [in contig]
Location63970..65478 [in whole cluster]
63970..65478 [in contig]
TypeCDS
Length1,509 bp (502 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category5.1 general function
Productputative glycosidase
Product (GenBank)beta-mannanase
Gene
Gene (GenBank)
EC number3.2.1.-
Keyword
Note
Note (GenBank)
  • Orf6
Reference
ACC
PmId
[12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002)
[15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004)
comment
[PMID: 12039053](2002)
Soraphen A biosynthetic gene clusterの報告。
Orf6(502aa): beta-mannanase

---
[PMID: 15289572](2004)
clusterの境界について検討。
C末側は配列解析(orf5はhypoで、orf6,7が一次代謝遺伝子）と、Sor. cellulosum So ce26 SJ3での遺伝子破壊でsoraphen類似体産生に影響しなかったことから、orf5-7はsor clusterでなはいと述べている。
Related Reference
ACC
O30654
PmId
[10525841] Sequencing and expression of a beta-mannanase gene from the extreme thermophile Dictyoglomus thermophilum Rt46B.1, and characteristics of the recombinant enzyme. (Curr Microbiol. , 1999)
comment
12th(O30654) 35%, 1e-47
Dictyoglomus thermophilum_manA
Beta-mannanase(EC 3.2.1.78)

manA過剰発現・精製、活性確認

close this sectionSequence

selected fasta
>putative glycosidase [beta-mannanase]
MRTSAWSYVSLSIALMGPAGCVVEADADLDAEVDELAGDLSTSTTYTLVGVQSGRCVDVP
GRSTADDIALNLWDCSGGTNQQFRFEAVSGGYYRIRNVNSNKCLDVRGASTADGAAIVQY
TCHNNTNQQWTVTDLGSDKVRITSRLSGKVIDAYGSPPENGTALFQWPSSGGTNQQFTLR
AVGTGTGTGTGTGTGTGTTDPTGPAPVSPSASPQARRLLSYIYSVYGNHILSGQHESTWV
GGPEYEMNYIHDRTGKYPAIRGLDFGDSGTSAASRAIAWWNAGGIPMIGYHMGAPTKPDG
YAGSQMTVSINAVLTSGTAENRSFHQRLDFAAGELQKLEDANVAVLWRPFHEAGGTWFWW
SKEGGAQYVRLWRYMFNYFTRDKGLTNLVWLHPYSGDPQASFYPGKEFVDITGADTYVSD
HNPLKAMFDRVRGIAGTTIPIALHENGPIPDPDQLEAQGARWVLFNTWHTDWLTGGSNPD
DFLRKVYGHRYVVTRDEVPNLR
selected fasta
>putative glycosidase [beta-mannanase]
ATGCGCACCTCCGCTTGGTCTTACGTTTCCCTGTCGATCGCGCTCATGGGCCCCGCCGGC
TGCGTCGTGGAGGCCGACGCCGACCTCGACGCGGAGGTCGACGAGCTCGCGGGCGACCTC
TCGACGAGCACCACCTACACCCTGGTCGGCGTCCAGAGCGGGCGCTGCGTCGACGTCCCA
GGCCGCTCCACGGCGGACGATATCGCGTTGAATCTCTGGGACTGCAGCGGCGGGACGAAC
CAGCAGTTCCGCTTCGAGGCGGTCTCCGGCGGCTATTACCGGATCCGCAACGTGAACAGC
AACAAGTGCCTCGACGTCCGCGGCGCGTCGACCGCCGACGGTGCCGCCATCGTCCAGTAC
ACCTGCCATAACAACACGAACCAGCAGTGGACCGTCACCGACCTCGGCAGCGACAAGGTC
CGCATCACCTCGCGCCTGAGCGGCAAGGTCATCGACGCGTACGGCTCCCCGCCCGAAAAC
GGCACCGCGCTGTTCCAGTGGCCCTCCAGCGGCGGCACGAACCAGCAGTTCACGCTGCGC
GCGGTGGGCACAGGTACCGGAACCGGAACCGGAACCGGTACAGGCACCGGAACCACCGAC
CCCACCGGGCCCGCGCCCGTGTCGCCCAGCGCCTCTCCGCAGGCGAGGCGGTTGCTCAGC
TACATCTACAGCGTGTACGGCAACCACATCCTCTCGGGGCAGCATGAGTCCACCTGGGTC
GGCGGGCCCGAGTACGAGATGAACTACATCCACGACCGCACGGGCAAATACCCCGCGATC
CGCGGCCTCGATTTCGGGGACTCCGGGACGTCCGCCGCCAGCCGCGCCATCGCCTGGTGG
AACGCGGGCGGCATTCCCATGATCGGCTACCACATGGGGGCGCCGACGAAGCCCGACGGC
TACGCCGGGTCCCAGATGACGGTGTCCATCAACGCCGTGCTCACGTCGGGGACGGCGGAG
AACCGATCGTTCCACCAGCGGCTCGACTTCGCCGCGGGCGAGCTGCAGAAGCTCGAGGAC
GCCAACGTGGCCGTGTTGTGGAGGCCGTTCCACGAGGCGGGCGGCACCTGGTTCTGGTGG
AGCAAGGAGGGCGGCGCGCAGTACGTCCGGCTGTGGAGGTACATGTTCAACTACTTCACC
CGGGACAAGGGCCTCACCAACCTGGTCTGGCTGCACCCCTATAGCGGCGACCCCCAGGCG
TCGTTCTACCCGGGCAAGGAGTTCGTCGACATCACCGGCGCGGACACGTATGTCAGCGAC
CACAACCCGCTGAAGGCGATGTTCGACAGGGTGCGCGGCATCGCGGGGACGACCATCCCC
ATCGCGCTCCACGAGAACGGACCCATCCCGGATCCCGATCAGCTGGAGGCCCAGGGCGCC
CGCTGGGTCCTCTTCAACACCTGGCACACCGACTGGTTGACCGGGGGGAGCAACCCGGAC
GACTTCCTGCGGAAGGTCTACGGCCACCGCTACGTCGTCACGCGCGACGAGGTGCCGAAC
CTGAGGTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000772 Ricin B lectin domain (Domain)
[43-177] 2.8e-25 PF00652
PF00652   Ricin_B_lectin
[42-180] PS50231
PS50231   RICIN_B_LECTIN
[41-179] 1.49999648196323e-40 SSF50370
SSF50370   RicinB_like
[43-180] 3.00000067992871e-27 SM00458
SM00458   RICIN
IPR000805 Glycoside hydrolase, family 26 (Family)
[278-292] 2.10000267834031e-18 PR00739 [344-361] 2.10000267834031e-18 PR00739 [371-394] 2.10000267834031e-18 PR00739 [405-417] 2.10000267834031e-18 PR00739
PR00739   GLHYDRLASE26
IPR013781 Glycoside hydrolase, catalytic domain (Domain)
[205-501] 5.90000000000008e-79 G3DSA:3.20.20.80
G3DSA:3.20.20.80   Glyco_hydro_cat
IPR017853 Glycoside hydrolase, superfamily (Domain)
[204-495] 1.3000049540733e-58 SSF51445
SSF51445   Glyco_hydro_cat
IPR022790 Endoglucanase H/Glycosyl hydrolase family 26 (Family)
[207-497] 1.69999999999998e-69 PF02156
PF02156   Glyco_hydro_26
SignalP
[1-25] 0.931 Signal
Bacteria, Gram-positive   
[1-24] 0.953 Signal
Bacteria, Gram-negative   
[1-21] 0.976 Signal
Eukaryota   
TMHMM No significant hit
Soraph_00110 Soraph_00110   Soraph_00130 Soraph_00130
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