Spino_00070 : CDS information

Location

Organism	Saccharopolyspora spinosa
Strain	NRRL 18538
Entry name	Spinosyn
Contig	AY007564
Start / Stop / Direction	8,967 / 10,427 / + [in whole cluster] 8,967 / 10,427 / + [in contig]
Location	8967..10427 [in whole cluster] 8967..10427 [in contig]
Type	CDS
Length	1,461 bp (486 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase
Product (GenBank)	probable NDP-hexose-2,3-dehydratase
Gene
Gene (GenBank)	spnO
EC number
Keyword	D-forosamine
Note
Note (GenBank)	involved in forosamine biosynthesis
Reference	ACC Q9ALN6 PmId [11358695] Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa. (Chem Biol. , 2001) [11386361] A cluster of genes for the biosynthesis of spinosyns, novel macrolide insect control agents produced by Saccharopolyspora spinosa. (Antonie Van Leeuwenhoek. , 2000) [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment [PMID: 11358695](2001) spinosad biosynthetic gene clusterの報告。 spnO(487aa): forosamine; 2,3-dehydratase Forosamine合成の初期段階でNDP-4-keto-6-deoxy-glucoseの2,3位の脱水反応を行う。 -- [PMID:11386361](2000) 同じ著者らの先行論文。 spnO: Forosamine synthesis --- [PMID: 18345667](2008) 使用している株はNRRL 18537. dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase [probable NDP-hexose-2,3-dehydratase]
MSSSVEAEASAAAPLGSNNTRRFVDSALSACNGMIPTTEFHCWLADRLGENSFETNRIPF
DRLSKWKFDASTENLVHADGRFFTVEGLQVETNYGAAPSWHQPIINQAEVGILGILVKEI
DGVLHCLMSAKMEPGNVNVLQLSPTVQATRSNYTQAHRGSVPPYVDYFLGRGRGRVLVDV
LQSEQGSWFYRKRNRNMVVEVQEEVPVLPDFCWLTLGQVLALLRQDNIVNMDTRTVLSCI
PFHDSATGPELAASEEPFRQAVARSLSHGIDSSSISEAVGWFEEAKARYRLRATRVPLSR
VDKWYRTDTEIAHQDGKYFAVIAVSVSATNREVASWTQPMIEPREQGEIALLVKRIGGVL
HGLVHARVEAGYKWTAEIAPTVQCSVANYQSTPSNDWPPFLDDVLTADPETVRYESILSE
EGGRFYQAQNRYRIIEVHEDFAARPPSDFRWMTLGQLGELLRSTHFLNIQARSLVASLHS
LWALGR

>dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase [probable NDP-hexose-2,3-dehydratase]
ATGAGCAGTTCTGTCGAAGCTGAGGCAAGTGCTGCTGCGCCGCTCGGCAGCAACAACACG
CGGCGGTTCGTCGACTCTGCGCTGAGCGCTTGCAATGGCATGATTCCGACCACGGAGTTC
CACTGCTGGCTCGCCGATCGGCTGGGCGAGAACAGCTTCGAGACCAATCGCATCCCGTTC
GACCGCCTGTCGAAATGGAAATTCGATGCCAGCACGGAGAACCTGGTTCATGCCGACGGT
AGGTTCTTCACGGTAGAAGGCCTGCAGGTCGAGACCAACTATGGCGCGGCACCCAGCTGG
CACCAGCCGATCATCAACCAGGCTGAAGTAGGTATCCTCGGCATTCTCGTCAAGGAGATC
GACGGCGTGCTGCACTGCCTCATGTCAGCAAAGATGGAACCGGGCAACGTCAACGTCCTG
CAGCTCTCGCCGACGGTTCAGGCAACTCGGAGCAACTACACGCAGGCACACCGTGGCAGC
GTTCCGCCCTATGTGGACTACTTCCTCGGGCGGGGCCGCGGCCGCGTGCTGGTAGACGTG
CTCCAGTCTGAACAGGGGTCCTGGTTCTACCGGAAGCGCAACCGGAACATGGTGGTGGAA
GTCCAGGAGGAAGTGCCAGTCCTGCCAGACTTCTGCTGGTTGACGCTCGGCCAGGTGCTG
GCTCTCCTTCGTCAGGACAACATCGTCAACATGGACACCCGGACGGTGCTGTCTTGCATC
CCGTTCCACGATTCCGCCACCGGACCCGAACTAGCCGCCTCGGAGGAGCCCTTCCGACAG
GCGGTGGCCAGGTCGCTCTCGCACGGCATCGATTCGTCGAGTATCTCCGAGGCGGTCGGT
TGGTTCGAGGAAGCCAAGGCCCGCTACCGCTTGCGGGCAACGCGCGTTCCGCTGAGCAGG
GTCGACAAGTGGTATCGCACCGATACCGAGATCGCCCACCAGGACGGCAAGTACTTCGCG
GTGATCGCGGTGTCGGTGTCCGCGACCAATCGTGAGGTCGCCAGCTGGACGCAGCCGATG
ATCGAACCGCGAGAACAAGGTGAGATCGCACTGTTGGTCAAGCGGATCGGCGGAGTGCTG
CACGGTTTGGTCCACGCTCGGGTGGAGGCTGGGTATAAGTGGACTGCGGAAATCGCTCCC
ACGGTCCAGTGCAGTGTGGCCAACTACCAAAGCACCCCGTCGAACGACTGGCCGCCGTTC
TTGGACGACGTGCTCACCGCCGATCCCGAAACCGTGCGGTACGAATCGATCCTGTCCGAA
GAAGGCGGTCGGTTCTACCAGGCGCAGAACAGGTACCGGATCATCGAGGTGCATGAGGAC
TTCGCGGCACGACCTCCCAGCGACTTCCGGTGGATGACTTTGGGACAGTTGGGCGAGCTG
CTCCGGAGCACCCACTTCTTGAACATCCAGGCGCGCAGCTTGGTCGCCTCCCTGCATAGC
TTGTGGGCGTTGGGGCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Spino_00060 Spino_00080