Spino_00070 Spino_00070   Spino_00090 Spino_00090

Spino_00080 : CDS information

close this sectionLocation

Organism
StrainNRRL 18538
Entry nameSpinosyn
Contig
Start / Stop / Direction10,436 / 11,434 / + [in whole cluster]
10,436 / 11,434 / + [in contig]
Location10436..11434 [in whole cluster]
10436..11434 [in contig]
TypeCDS
Length999 bp (332 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductdTDP-hexose C-3 ketoreductase
Product (GenBank)probable NDP-hexose-3-ketoreductase
Gene
Gene (GenBank)spnN
EC number
Keyword
  • D-forosamine
Note
Note (GenBank)
  • involved in forosamine biosynthesis
Reference
ACC
PmId
[11358695] Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa. (Chem Biol. , 2001)
[11386361] A cluster of genes for the biosynthesis of spinosyns, novel macrolide insect control agents produced by Saccharopolyspora spinosa. (Antonie Van Leeuwenhoek. , 2000)
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
[PMID: 11358695](2001)
spinosad biosynthetic gene clusterの報告。
spnN(333aa): forosamine; 3-ketoreductase

Forosamine合成過程で、3位のケト基の還元に関与する。

--
[PMID: 11386361](2000)
同じ著者らの先行論文。
spnN: Forosamine synthesis

---
[PMID: 18345667](2008)
使用している株はNRRL 18537.
NAD(P)H-dependent dTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductase_SpnN

dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), TylX3(SpnNの1つ前の反応を担うSpnOのhomologue), SpnN, and NADPHをインキュベーションしてできた産物がdTDP-4-keto-2,6-dideoxy-D-glucoseであることをHPLC chromatogram、1H NMR spectrumなどで確認している。NADPHはNADHの2倍速く消費されるので、おそらくNADPH-dependentである。

close this sectionSequence

selected fasta
>dTDP-hexose C-3 ketoreductase [probable NDP-hexose-3-ketoreductase]
MRKPVRIGVLGCASFAWRRMLPAMCDVAETEVVAVASRDPAKAERFAARFECEAVLGYQR
LLERPDIDAVYVPLPPGMHAEWIGKALEADKHVLAEKPLTTTASDTARLVGLARRKNLLL
RENYLFLHHGRHDVVRDLLQSGEIGELREFTAVFGIPPLPDTDIRYRTELGGGALLDIGV
YPARAARHFLLGPLTVLGASSHEAQESGVDLSGSVLLQSEGGTVAHLGYGFVHHYRSAYE
LWGSRGRIVVDRAFTPPAEWQAVIRIERKGVVDELSLPAEDQVRKAVTAFARDIRAGTGV
DDPAVAGDSGESMIQQAALVEAIGQARRCGST
selected fasta
>dTDP-hexose C-3 ketoreductase [probable NDP-hexose-3-ketoreductase]
ATGCGAAAGCCGGTGCGCATCGGTGTGCTCGGGTGCGCTTCCTTCGCGTGGCGACGGATG
CTGCCCGCGATGTGCGACGTGGCCGAAACAGAGGTGGTGGCGGTGGCGAGCCGTGATCCG
GCGAAAGCCGAACGGTTCGCAGCGCGATTCGAATGCGAGGCGGTGCTGGGTTACCAGCGG
CTCCTGGAGCGGCCGGACATCGATGCCGTCTACGTGCCGTTGCCGCCTGGCATGCATGCA
GAGTGGATCGGCAAGGCGCTTGAGGCAGACAAACACGTGCTTGCGGAGAAACCGCTGACG
ACGACGGCGTCCGACACCGCTCGCCTGGTCGGGCTGGCCAGGAGGAAGAACCTGCTGCTG
CGGGAGAATTACCTGTTCCTCCACCACGGCCGGCACGACGTGGTCCGCGACCTGCTGCAA
TCCGGGGAGATCGGTGAGCTCCGGGAGTTCACCGCCGTGTTCGGAATTCCGCCGCTTCCC
GACACGGACATCCGCTATCGCACCGAACTCGGTGGCGGAGCGTTGCTGGACATCGGTGTC
TATCCCGCCCGTGCCGCTCGGCACTTTCTCCTCGGTCCGCTCACGGTTCTCGGCGCAAGC
TCGCACGAGGCCCAGGAGTCGGGCGTCGACTTGTCGGGCAGCGTGCTGCTCCAATCGGAA
GGTGGCACCGTTGCCCACCTCGGATACGGTTTCGTGCACCACTACCGCAGCGCGTACGAG
CTGTGGGGGAGTCGTGGGCGAATCGTCGTCGACCGGGCGTTCACGCCGCCCGCCGAGTGG
CAGGCCGTGATCCGAATCGAGCGGAAGGGCGTTGTCGACGAGTTGTCCTTGCCAGCGGAA
GATCAGGTTCGCAAGGCGGTCACCGCCTTCGCACGCGACATCAGAGCAGGGACAGGCGTG
GACGACCCTGCGGTGGCCGGAGATTCGGGCGAATCGATGATCCAGCAGGCCGCGCTGGTG
GAGGCGATCGGTCAGGCCCGTCGGTGCGGGTCCACATAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000683 Oxidoreductase, N-terminal (Domain)
[5-123] 4.10000000000001e-27 PF01408
PF01408   GFO_IDH_MocA
IPR004104 Oxidoreductase, C-terminal (Domain)
[136-239] 2.3e-05 PF02894
PF02894   GFO_IDH_MocA_C
IPR016040 NAD(P)-binding domain (Domain)
[1-145] 2.50000000000001e-41 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-22] 0.887 Signal
Eukaryota   
[1-23] 0.406 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Spino_00070 Spino_00070   Spino_00090 Spino_00090
Page top