Vicen_00100 : CDS information

Location

Organism	Streptomyces halstedii
Strain	HC34
Entry name	Vicenistatin
Contig	AB086653
Start / Stop / Direction	35,332 / 36,801 / + [in whole cluster] 35,332 / 36,801 / + [in contig]
Location	35332..36801 [in whole cluster] 35332..36801 [in contig]
Type	CDS
Length	1,470 bp (489 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose 2,3-dehydratase
Product (GenBank)	dTDP-4-keto-6-deoxyglucose 2,3-dehydratase
Gene
Gene (GenBank)	vinD
EC number
Keyword	vicenisamine
Note
Note (GenBank)
Reference	ACC Q76KZ0 PmId [15112997] Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. (Chem Biol. , 2004) comment vicenistatin生合成gene clusterの同定論文。 VinD (489aa) : dTDP-glucose 2,3-dehydratase 配列解析からの機能推定。 vicenistatinに含まれるアミノ糖vicenisamine生合成の3番目のステップ。 dTDP-4-keto-6-deoxyglucoseで2,3位の脱水反応を仲介し、dTDP-3,4-diketo-2,6-dideoxyglucoseを得る。
Related Reference	ACC Q9ALN6 NITE Spino_00070 PmId [18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008) comment 22nd(Q9ALN6) 52%, 1e-125 Saccharopolyspora spinosa_spnO Probable NDP-hexose-2,3-dehydratase [Spino_00070]dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase Forosamine合成の初期段階で NDP-4-keto-6-deoxy-glucoseの2,3位の脱水反応を行う。 --- [PMID: 18345667] dTDP-4-keto-6-deoxy-D-glucose 2,3-dehydratase_SpnO dTDP-D-glucose, RfbB(dTDP-D-glucose→dTDP-4-keto-6-deoxy-D-glucose), and either NADH or NADPHと一緒にSpnO and SpnNをインキュベートし、NAD(P)Hの消費量をフォローしてtandem reactionを測定。SpnO or SpnNのどちらかを欠いた場合、消費が見られなかった。 dTDP-3,4-diketo-2,6-dideoxy-D-glucoseは不安定で急速にdTDP and maltolに分解してしまうため、SpnOのみの触媒効率は定量できていない。 -- SpnNはdTDP-3,4-diketo-2,6-dideoxy-D-glucose 3-ketoreductaseであることが他で報告されている。

Sequence

>putative NDP-hexose 2,3-dehydratase [dTDP-4-keto-6-deoxyglucose 2,3-dehydratase]
MTETLSTLPPTHTGDESLAVRIAKSVSVVDDGALHSLDEFHDWFTARGQRTAHVERVPLD
ELTGWSSDPVTGNIGHDSGKFFSVQGLSVELPGAPVPAWAQPIINQPEIGILGILIKEFN
GVLHCLMQAKLEPGNRNGLQLSPTVQATRSNYTRVHQGNPVPYVGYFQDTSANRVITDAL
QSEQGSWFYQKRNRNMVVETEAEIEQHEAFMWLTIGQLHRLLAIDDLINMDTRTVLSCLP
FSGAQLAEQLPGTGGDLRMPILRSCSEDQGSLHTTGDLLSWITDARTRNEVRTRLTPLRD
VAGWRRTPDAITHDTGRFFDVMAVRIETDGDREVRQWTQPMIAPAGIGIVAFLVKRIDGV
LHVLAHARVEPGYLDVVELSPTVMCTPGNYEGLPAAARPPFLGEVLSARPEQVRFETILS
EEGGRFYHAQNRYIIVESDIDVAPELASEYRWMALHQMVGLLRHSHYVNVQARSLIACLH
SLSGAQGRN

>putative NDP-hexose 2,3-dehydratase [dTDP-4-keto-6-deoxyglucose 2,3-dehydratase]
ATGACCGAGACGCTGTCGACTCTTCCGCCCACCCACACCGGCGACGAGTCGCTCGCGGTC
CGTATCGCCAAGTCGGTGAGTGTCGTCGACGACGGGGCGCTGCACAGCCTCGACGAATTC
CACGACTGGTTCACCGCCAGAGGGCAGCGGACCGCACATGTCGAGAGAGTGCCGCTCGAC
GAACTCACTGGTTGGAGCTCCGATCCCGTGACCGGGAACATCGGGCACGACAGTGGGAAG
TTCTTCAGTGTGCAGGGGCTCTCCGTCGAACTGCCCGGTGCGCCGGTGCCCGCCTGGGCG
CAGCCCATCATCAATCAGCCCGAGATCGGGATCCTGGGGATTCTGATCAAGGAATTCAAC
GGTGTTCTGCACTGTCTGATGCAGGCGAAGCTGGAGCCGGGGAACCGCAACGGCCTCCAG
CTCTCCCCGACGGTGCAGGCGACCCGGAGCAACTACACCCGGGTGCACCAGGGCAACCCC
GTCCCGTACGTGGGCTACTTCCAGGACACCTCGGCGAACCGGGTGATCACCGACGCGCTC
CAGTCCGAACAGGGCTCGTGGTTCTACCAGAAGCGCAACCGGAACATGGTCGTCGAGACC
GAGGCGGAGATCGAGCAGCACGAGGCGTTCATGTGGCTGACCATCGGTCAGCTGCACCGC
CTCCTTGCGATCGACGACCTGATCAACATGGACACCCGTACGGTGCTGTCGTGTCTGCCC
TTCTCCGGTGCGCAGCTCGCCGAGCAGCTCCCGGGCACCGGCGGCGATCTGCGGATGCCG
ATCCTGCGCTCGTGCAGCGAGGACCAGGGCAGTCTGCACACCACCGGGGACCTGCTGAGC
TGGATCACTGACGCCCGTACCCGCAACGAGGTGCGCACCCGGCTGACGCCGCTGCGCGAC
GTGGCGGGCTGGCGGCGCACCCCGGACGCGATCACGCACGACACGGGTCGGTTCTTCGAC
GTGATGGCCGTGCGCATCGAGACCGACGGGGACCGCGAGGTCCGCCAGTGGACCCAGCCG
ATGATCGCGCCGGCCGGGATCGGCATCGTGGCGTTCCTGGTCAAGCGGATCGACGGGGTG
CTGCACGTGCTGGCGCACGCCCGGGTCGAGCCGGGCTATCTCGATGTCGTGGAGCTGTCG
CCCACGGTCATGTGCACGCCCGGCAACTACGAGGGCCTGCCCGCCGCCGCCCGGCCGCCG
TTCCTCGGCGAGGTGCTCTCGGCCCGTCCCGAGCAGGTCCGCTTCGAGACGATCCTCTCC
GAGGAGGGCGGCCGGTTCTACCACGCGCAGAACCGGTACATCATCGTCGAGTCCGATATC
GACGTGGCGCCGGAACTGGCCTCGGAGTACCGCTGGATGGCGCTCCACCAGATGGTCGGC
CTGCTGCGGCACAGCCACTACGTCAACGTCCAGGCCCGCAGTCTGATCGCGTGTCTGCAC
AGCCTCTCGGGGGCGCAGGGGCGGAACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005212 NDP-hexose 2,3-dehydratase (Domain) PF03559 Hexose_dehydrat
SignalP	No significant hit
TMHMM	No significant hit

Vicen_00090 Vicen_00110