Vicen_00100 Vicen_00100   Vicen_00120 Vicen_00120

Vicen_00110 : CDS information

close this sectionLocation

Organism
StrainHC34
Entry nameVicenistatin
Contig
Start / Stop / Direction36,855 / 37,841 / + [in whole cluster]
36,855 / 37,841 / + [in contig]
Location36855..37841 [in whole cluster]
36855..37841 [in contig]
TypeCDS
Length987 bp (328 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-3 ketoreductase
Product (GenBank)dTDP-4-keto-6-deoxyhexose 2,3-reductase
Gene
Gene (GenBank)vinE
EC number
Keyword
  • vicenisamine
  • axial-OH
Note
Note (GenBank)
Reference
ACC
PmId
[15112997] Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. (Chem Biol. , 2004)
comment
vicenistatin生合成gene clusterの同定論文。

VinE (328aa) : dTDP-hexose 2,3-reductase

配列解析からの機能推定。
vicenistatinに含まれるアミノ糖vicenisamine生合成の4番目のステップ。
dTDP-3,4-diketo-2,6-dideoxyglucoseのC-3 keto基をOH基に還元する。
Related Reference
ACC
Q9XC70
PmId
[16448097] A two-stage one-pot enzymatic synthesis of TDP-L-mycarose from thymidine and glucose-1-phosphate. (J Am Chem Soc. , 2006)
comment
4th(Q9XC70) 62%, 1e-115
Streptomyces fradiae_tylCII(tylC1)
NDP-hexose 2,3-enoyl reductase TylCII

[PMIDなし]Biosynthesis of Mycarose: Isolation and Characterization of Enzymes Involved in the C-2 Deoxygenation. J Am Chem Soc 1999

TylX3をdTDP-D-glucoseを4,6-dehydrataseで処理した基質と反応するとmaltol産生。これは予測された不安定なdehydration産物の分解の結果であると示唆される。

TylX3にTylC1, NADPHを加えて反応すると、C-3 OH基がaxialなdTDP-2,6-dideoxy-D-glycero-D-glycero-4-hexuloseを産生。
この産物のC-2のHはequatrial positionであることが重水素の取り込みで確認されたので、C-2の立体配置は保持されており、水素化物がNADPHから不安定中間体のC-3 positionへ移されることがほのめかされる。

--
[PMID:16448097]上記論文の引用あり。

close this sectionSequence

selected fasta
>putative NDP-hexose C-3 ketoreductase [dTDP-4-keto-6-deoxyhexose 2,3-reductase]
MLYRQLGRTALKVSPLCLGTLNLGVRTTRDEAFALMDEALEQGINFFDTANQYGWQVHKG
LTEEIIGEWFAQGGGRRERVVLGTKVCNPMSDLPNDQGLSARHIIASCEDSLRRLRTDWI
DVYQLHNIDPTASWDEVWQAMETLVHQGKIRYVGSSNFAGWHLADAQAAAARRNFLGLVS
EQCCYNLATRYVEMEVVPAARAHGIGVLAWSPLHGGLLSGALRKLDEGTAVKTAQGRAQI
ALRTMRDTVEQYEKLCESISADPSQVALAWLLSRPGLTAAVIGPRTTGHLASALETLDMT
LPEDILAPLDELFPPIGNGQPAPATWLA
selected fasta
>putative NDP-hexose C-3 ketoreductase [dTDP-4-keto-6-deoxyhexose 2,3-reductase]
ATGCTCTACCGGCAGCTCGGACGTACGGCACTGAAGGTCAGCCCGCTCTGTCTGGGCACC
CTCAACCTCGGCGTCCGTACCACCCGTGACGAGGCGTTCGCCCTCATGGACGAGGCCCTG
GAGCAGGGCATCAACTTCTTCGACACCGCCAACCAGTACGGCTGGCAGGTCCACAAGGGC
CTGACGGAGGAGATCATCGGAGAGTGGTTCGCACAGGGCGGCGGCCGGCGCGAGCGCGTG
GTCCTGGGCACCAAGGTCTGCAACCCGATGAGCGATCTGCCCAACGACCAGGGGCTGTCG
GCGCGGCACATCATCGCCTCCTGCGAGGACTCGCTACGGCGGCTGCGCACCGACTGGATC
GATGTCTACCAGCTCCACAACATCGACCCGACGGCGTCCTGGGACGAGGTGTGGCAGGCC
ATGGAGACCCTGGTGCACCAGGGCAAGATCCGTTACGTGGGGTCGTCCAACTTCGCGGGC
TGGCATCTGGCCGACGCGCAGGCCGCGGCGGCCCGCCGTAACTTCCTCGGACTCGTGTCG
GAGCAGTGCTGCTACAACCTGGCGACCCGGTACGTGGAGATGGAGGTCGTCCCCGCCGCG
CGGGCGCACGGCATCGGAGTGCTGGCCTGGTCGCCGCTGCACGGCGGTCTGCTGAGCGGG
GCGCTGCGCAAGCTGGACGAGGGGACGGCGGTGAAGACGGCCCAGGGCCGTGCGCAGATC
GCGCTGCGGACCATGCGGGACACCGTCGAGCAGTACGAGAAGCTGTGCGAGAGCATCTCC
GCCGACCCGTCCCAGGTTGCCCTGGCCTGGCTGCTGTCCCGGCCGGGTCTCACGGCCGCC
GTCATCGGCCCGCGCACCACCGGGCACCTGGCGAGCGCGCTGGAGACCCTGGACATGACG
CTGCCCGAGGACATCCTGGCGCCGCTGGACGAACTCTTCCCGCCGATCGGCAACGGTCAG
CCGGCGCCGGCCACCTGGCTCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR023210 NADP-dependent oxidoreductase domain (Domain)
[15-313] 1.20000000000001e-69 PF00248
PF00248   Aldo_ket_red
[1-318] 1.79999754022378e-93 SSF51430
SSF51430   Aldo/ket_red
[11-315] 1.10000000000001e-89 G3DSA:3.20.20.100
G3DSA:3.20.20.100   Aldo/ket_red
SignalP
[1-34] 0.39 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Vicen_00100 Vicen_00100   Vicen_00120 Vicen_00120
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