Vicen_00190 : CDS information

Location

Organism	Streptomyces halstedii
Strain	HC34
Entry name	Vicenistatin
Contig	AB086653
Start / Stop / Direction	45,589 / 46,833 / + [in whole cluster] 45,589 / 46,833 / + [in contig]
Location	45589..46833 [in whole cluster] 45589..46833 [in contig]
Type	CDS
Length	1,245 bp (414 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	3-methylaspartyl-PCP decarboxylase
Product (GenBank)	decarboxylase
Gene
Gene (GenBank)	vinO
EC number	4.1.1.-
Keyword
Note
Note (GenBank)
Reference	ACC Q76KY1 PmId [15112997] Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. (Chem Biol. , 2004) [22010945] A natural protecting group strategy to carry an amino acid starter unit in the biosynthesis of macrolactam polyketide antibiotics. (J Am Chem Soc. , 2011) comment [PMID: 15112997](2004) vicenistatin生合成gene clusterの同定論文。配列解析からの機能推定。 Starter unit合成の中で(2S,3S)-3-methylaspartateのカルボキシル基を除去する。 --- [PMID: 22010945](2011) VinJ, K, L, M, N, Oの酵素的解析を行っている論文。 HPLCで精製した3-methylaspartate(3-MeAsp)-VinLをVinOで反応させて、mixtureの産物をLC-ESI-MS解析。decarboxylationされた3-amino-2-methylpropionyl-VinLが形成されていることを確認している。（ただし、retention timeは3-MeAsp-VinLと似ている。）また、PLP-dependentな酵素であるVinOは3-amino-2-methylpropionyl-VinLのC-2 positionでの異性化も同時に触媒すると提唱されるが、確認はできていない。

Sequence

>3-methylaspartyl-PCP decarboxylase [decarboxylase]
MNQYEELADQYGTPLYVYDLDRVAEARHDLRTSLPDEVEIYYALKANPHPQVAGALRSGE
GRECRAEISSVGELAAALTAGFRASEILYTGPGKTDGELDEAIGKGVKTFCVESLTDLQH
VGAVALRHGVVADCLLRINSATASATTSIRMTGTPSQFGIDSETLVDAMPELRAVPGTRI
TGLHFFPLSNARDEASLIGEFRHTIAYAAGLAEETGLTLEFLDIGGGFAHPYGAPGERPV
YRELRTELAAALDEHFPHWREGAPRIAVETGRYQTSGAGTLLTRVVNIKVSRGRKFVVID
AGINTFGGMSGLGRLLPVAVEPEYTGSAEATELTDVASLAGPLCTPGDILGREIRLPELA
PGDLVTIPNAGAYGVTASLLMFLGRPAPVEVVLKGGKVVSASRLEHHRTPATPG

>3-methylaspartyl-PCP decarboxylase [decarboxylase]
GTGAACCAGTACGAAGAACTCGCCGACCAGTACGGCACCCCGCTCTACGTCTACGACCTC
GACCGCGTCGCCGAGGCCCGGCACGATCTGCGCACCTCACTGCCCGACGAGGTCGAGATC
TACTACGCCCTCAAGGCCAACCCCCACCCGCAGGTCGCCGGCGCCCTGCGCAGCGGCGAG
GGCCGCGAGTGCCGCGCCGAGATCAGCTCCGTCGGCGAGCTGGCCGCCGCCCTCACCGCC
GGCTTCCGTGCCTCCGAGATCCTCTACACCGGCCCCGGCAAGACCGACGGCGAACTGGAC
GAAGCGATCGGCAAGGGCGTCAAGACCTTCTGCGTCGAATCGCTGACCGACCTCCAGCAC
GTCGGCGCCGTCGCCCTGCGACACGGCGTCGTCGCCGACTGCCTGCTGCGCATCAACAGC
GCCACCGCCAGCGCCACCACCAGCATCCGGATGACCGGAACCCCCTCACAGTTCGGCATC
GACAGCGAGACCCTCGTCGACGCCATGCCCGAACTGCGCGCCGTACCCGGCACCCGGATC
ACGGGCCTGCACTTCTTCCCCCTCAGCAACGCGAGGGACGAGGCCAGCCTCATCGGCGAG
TTCCGCCACACCATCGCCTACGCGGCCGGCCTCGCCGAGGAGACCGGTCTCACCCTGGAA
TTCCTCGACATCGGCGGCGGCTTCGCCCACCCCTACGGCGCGCCCGGCGAGCGCCCCGTC
TACCGCGAACTCCGCACCGAGCTGGCCGCCGCCCTCGACGAGCACTTCCCGCACTGGCGC
GAGGGCGCCCCGCGCATCGCGGTGGAGACCGGCCGCTACCAGACCAGCGGCGCCGGAACC
CTGCTCACCCGGGTCGTCAACATCAAGGTCAGCCGGGGCCGGAAGTTCGTCGTCATCGAC
GCCGGGATCAACACCTTCGGCGGTATGTCCGGCCTCGGCCGGCTGCTGCCGGTCGCCGTC
GAACCCGAGTACACCGGCTCCGCCGAAGCCACCGAACTCACCGATGTCGCCAGCCTCGCC
GGCCCCCTCTGCACCCCCGGTGACATCCTCGGCCGCGAGATCCGCCTGCCCGAACTGGCC
CCCGGCGACCTCGTGACCATCCCCAACGCGGGCGCGTACGGCGTCACCGCGAGCCTGCTG
ATGTTCCTCGGCAGGCCCGCGCCGGTCGAGGTCGTCCTGAAGGGCGGCAAGGTCGTCTCC
GCGTCCCGACTGGAGCACCACCGCACTCCGGCGACCCCCGGATAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR009006 Alanine racemase/group IV decarboxylase, C-terminal (Domain) G3DSA:2.40.37.10 Ala_racemase/Decarboxylase_C SSF50621 Racem_decarbox_C IPR022643 Orn/DAP/Arg decarboxylase 2, C-terminal (Domain) PF00278 Orn_DAP_Arg_deC IPR022644 Orn/DAP/Arg decarboxylase 2, N-terminal (Domain) PF02784 Orn_Arg_deC_N
SignalP	No significant hit
TMHMM	No significant hit

Vicen_00180 Vicen_00200