Vicen_00170 Vicen_00170   Vicen_00190 Vicen_00190

Vicen_00180 : CDS information

close this sectionLocation

Organism
StrainHC34
Entry nameVicenistatin
Contig
Start / Stop / Direction44,156 / 45,592 / + [in whole cluster]
44,156 / 45,592 / + [in contig]
Location44156..45592 [in whole cluster]
44156..45592 [in contig]
TypeCDS
Length1,437 bp (478 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Product3-methylaspartate--PCP ligase
3-methylaspartyl-PCP synthetase
Product (GenBank)non-ribosomal peptide synthetase
Gene
Gene (GenBank)vinN
EC number
Keyword
Note
  • VinN recognizes (2S,3S)-3-methylaspartate as a beta-amino acid substrate and adenylates the carboxyl group at the C4 position.
Note (GenBank)
Reference
ACC
PmId
[15112997] Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. (Chem Biol. , 2004)
[22010945] A natural protecting group strategy to carry an amino acid starter unit in the biosynthesis of macrolactam polyketide antibiotics. (J Am Chem Soc. , 2011)
[25246523] The crystal structure of the adenylation enzyme VinN reveals a unique beta-amino acid recognition mechanism. (J Biol Chem. , 2014)
comment
[PMID: 15112997](2004)
vicenistatin生合成gene clusterの同定論文。

配列解析からの機能推定。
Starter unit合成の際に、(2S,3S)-methylaspartateを活性化する酵素として働く。
NRPSのAドメインに相同性あり。

---
[PMID: 22010945](2011)
VinJ, K, L, M, N, Oの酵素的解析を行っている論文。

放出されたphyrophosphateを検出するPhotometric assayにより、ATP-dependent ligase VinM and VinNの基質特異性を確認。

VinMは、L-alanine, glycine, serineのような小さなAAを認識する。
VinNは、3-methylaspartate(3-MeAsp)にのみ特異的に触媒する。また、そのmixtureにholo型VinL(ACP)を加えると、3-MeAsp-VinLが形成されることをLC-ESI-MSで確認している。

よって、VinNは3-MeAspを活性化し、VinLへの移動を触媒する。

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[PMID: 25246523](2014)
β-アミノ酸基質として(2S,3S)-3-methylaspartate (3-MeAsp)を活性化する独立型adenylation酵素VinNを、3-MeAspまたはL-aspartateとの複合体で結晶構造解析。変異解析や、α-アミノ酸アデニル化酵素との配列・構造比較、系統解析もしている。

close this sectionPKS/NRPS Module

0
A9..410

close this sectionSequence

selected fasta
>3-methylaspartate--PCP ligase [non-ribosomal peptide synthetase]
MEDAFGTWTYQELLNHSQAFSAWLDGKGVARGERIVVQLPNIRQTVAVFYGACRRGVVFV
PLNPGMKPFHLRSVIADADPRLVIAEDETAADRLRDVTDLPVYSIDSLWADVERLRDAGA
GAEAVEVSPEDLAVLIYTSGSTAAPKAVACPHQQIVFAASSINAVLGYHAEDIVFCRMSV
SWDFGLYKVLISTLTGAKLVLAGGEPDIALVKSLRESGATMMPIVPSLASMLTTLIRRDP
EGAPTLRMFTNSAAALPQVTIDALRSAFPGAQVVRMYGQTECKRISIMPPHLEHERPDSV
GLPLPGTTIEILDEDGTLLPPGEPGEITVTGPHVMAGYWRAPEITARAYRRDETTGAMRL
HTGDYGHLDEDGFLYFGGRRDDMFKRKGTRMSTVEIEAAALDIPGVTAAVALPPTATRDL
ALCVASDLEPHDVLRSLAERLEPAKVPATCRIVNDFPLTLNGKSERKQLARLLDGSDK
selected fasta
>3-methylaspartate--PCP ligase [non-ribosomal peptide synthetase]
ATGGAAGACGCATTCGGAACCTGGACCTATCAAGAGCTGCTGAACCATAGTCAGGCATTC
AGCGCCTGGCTGGACGGAAAGGGCGTCGCCCGCGGCGAACGCATCGTCGTCCAGCTCCCG
AACATCCGCCAGACGGTCGCCGTGTTCTACGGAGCCTGCCGCCGCGGCGTCGTGTTCGTC
CCGCTCAACCCGGGCATGAAGCCCTTCCACCTGCGGTCCGTCATCGCCGACGCCGACCCG
CGCCTGGTCATCGCCGAGGACGAGACCGCGGCCGACCGGCTGCGTGACGTCACCGACCTG
CCGGTGTACTCCATCGACTCCCTCTGGGCGGATGTCGAGCGGCTGCGCGACGCGGGCGCC
GGAGCCGAGGCCGTCGAGGTCTCCCCGGAGGATCTGGCCGTCCTCATCTACACCTCCGGC
TCCACCGCGGCCCCCAAGGCGGTCGCCTGCCCCCACCAGCAGATCGTGTTCGCCGCCTCC
TCGATCAACGCCGTACTCGGCTACCACGCCGAAGACATCGTCTTCTGCCGGATGTCGGTC
TCCTGGGACTTCGGTCTCTACAAGGTGCTGATCTCCACCCTCACCGGCGCCAAGCTCGTC
CTCGCGGGCGGTGAGCCCGACATCGCGCTCGTCAAGTCCCTCCGCGAGAGCGGCGCCACG
ATGATGCCCATCGTGCCGTCCCTGGCGAGCATGCTCACCACCCTCATCCGGCGCGACCCC
GAGGGCGCCCCGACGCTGCGGATGTTCACCAACAGCGCCGCCGCGCTGCCCCAGGTCACC
ATCGACGCCCTGCGCTCCGCGTTCCCCGGCGCCCAGGTCGTGCGCATGTACGGCCAGACC
GAGTGCAAGCGCATCTCGATCATGCCGCCGCATCTGGAGCACGAGCGACCCGACTCCGTC
GGTCTGCCGCTGCCCGGCACCACCATCGAGATCCTGGACGAGGACGGCACCCTCCTGCCG
CCCGGTGAGCCCGGCGAGATCACCGTCACCGGACCCCATGTCATGGCCGGCTACTGGCGC
GCGCCCGAGATCACCGCCCGCGCCTACCGCCGCGACGAGACCACCGGCGCGATGCGCCTG
CACACCGGCGACTACGGCCACCTCGACGAGGACGGATTCCTCTACTTCGGCGGCCGGCGC
GACGACATGTTCAAGCGCAAGGGCACCCGGATGAGCACCGTCGAGATCGAGGCCGCGGCC
CTCGACATCCCCGGCGTCACCGCCGCCGTGGCGCTGCCCCCGACCGCCACCCGGGACCTG
GCCCTGTGTGTCGCGTCCGACCTCGAACCCCATGACGTCCTCCGCAGCCTCGCGGAGCGG
CTCGAACCGGCGAAGGTCCCCGCGACCTGCCGTATCGTCAACGACTTCCCGCTCACCCTG
AACGGCAAGTCCGAGCGCAAGCAGCTCGCACGCCTGCTCGACGGGAGCGACAAGTGA
[0] A9..410
[0] A25..1230

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000873 AMP-dependent synthetase/ligase (Domain)
[9-410] 5.80000000000004e-96 PF00501
PF00501   AMP-binding
SignalP No significant hit
TMHMM No significant hit
Vicen_00170 Vicen_00170   Vicen_00190 Vicen_00190
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