Vicen_00170 : CDS information

Location

Organism	Streptomyces halstedii
Strain	HC34
Entry name	Vicenistatin
Contig	AB086653
Start / Stop / Direction	42,289 / 43,863 / + [in whole cluster] 42,289 / 43,863 / + [in contig]
Location	42289..43863 [in whole cluster] 42289..43863 [in contig]
Type	CDS
Length	1,575 bp (524 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	3-amino-2-methylpropionyl-PCP--alanine ligase alanine-3-amino-2-methylpropionyl-PCP synthetase
Product (GenBank)	non-ribosomal peptide synthetase
Gene
Gene (GenBank)	vinM
EC number
Keyword	protection
Note
Note (GenBank)
Reference	ACC Q76KY3 PmId [15112997] Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. (Chem Biol. , 2004) [22010945] A natural protecting group strategy to carry an amino acid starter unit in the biosynthesis of macrolactam polyketide antibiotics. (J Am Chem Soc. , 2011) comment [PMID: 15112997](2004) vicenistatin生合成gene clusterの同定論文。 VinM (524aa) : nonribosomal peptide synthetase 配列解析からの機能推定。 Starter unit合成の際に、(2S,3S)-methylaspartateをActivationする酵素として働く。 NRPSのAドメインに相同性あり。 --- [PMID: 22010945](2011) VinJ, K, L, M, N, Oの酵素的解析を行っている論文。放出されたphyrophosphateを検出するPhotometric assayにより、ATP-dependent ligase VinM and VinNの基質特異性を確認。 VinMは、L-alanine, glycine, serineのような小さなAAを認識する。最も適しているのはL-alanine. 3-amino-2-methylpropionyl-VinLを、VinM, ATP, L-alanine(or glycine, serine)で反応させて、mixtureの産物をLC-ESI-MS解析。alanine(or 対応するAA)が付加された3-amino-2-methylpropionyl-VinLのシグナルが検出された。 VinN, L, O, Mと基質アミノ酸のone-pot reactionは、alanine-3-amino-2-methylpropionyl-VinLのみを産出した。

Sequence

>3-amino-2-methylpropionyl-PCP--alanine ligase [non-ribosomal peptide synthetase]
MHAADHALHARFLRGLACAPDRPAVRFGGRTLTYAQAHRTALTWAGSLLRATPEPPAAVG
VLADKGIPAYLGILTALYAGAAVVPLRPDFPAARTAEMMRAAGVTAVIADGRGRRLLPEL
LADRRDTAVLAADEEGAPADESPADGSAPGRRVAIDEGYALTAPRDVVPDDTAYVLFTSG
STGRPKGVPLSHGNIAHYFEVLDARYDFTADDVFTQTFDLNFDCSLFDLFCAWGAGASVI
QIPPQAYRDLPSHLAEQGVTVWFSTPSSIALVRRLGGLAPGSLPTLRWSFFAGEALKCAD
TEDWQRAAPASFVENLYGPTELTVTVTAHRWSPEVSPVVGANGVVPIGPLHKGLDHVLID
AGGLPHPDTGELCVTGPQMAGRYLDPADDHGRFLDHDGRRWYRTGDRVRLAPGGELVYLG
RMDAQVQIQGWRVELAEVDHALQGCEGVGEAVTVGAATDAGTELVVFYTAPAPVPPVRFA
AVLRATLPDGVVPRHYRHVAELPLNSNRKIDRRALTARAEELLG

>3-amino-2-methylpropionyl-PCP--alanine ligase [non-ribosomal peptide synthetase]
ATGCACGCCGCCGACCACGCCCTGCACGCCCGCTTCCTGCGCGGGCTCGCCTGCGCGCCC
GACCGGCCCGCCGTACGGTTCGGCGGGCGGACCCTGACGTACGCGCAGGCCCACCGCACC
GCCCTGACCTGGGCGGGTTCCCTGCTGCGCGCCACGCCGGAGCCCCCGGCCGCCGTCGGT
GTCCTCGCCGACAAGGGCATCCCCGCCTATCTGGGCATCCTGACCGCGCTCTACGCCGGC
GCCGCCGTCGTGCCGCTGCGGCCGGACTTCCCCGCGGCCCGTACCGCCGAGATGATGCGG
GCCGCGGGGGTCACCGCCGTCATAGCCGACGGTCGTGGCCGGCGGCTGCTCCCCGAACTG
CTCGCCGACCGCCGGGACACCGCCGTCCTGGCCGCCGACGAGGAAGGCGCCCCCGCCGAC
GAGAGCCCCGCCGACGGGAGCGCCCCGGGCCGCCGTGTCGCGATCGACGAGGGGTACGCG
CTCACCGCGCCCCGGGACGTCGTCCCGGACGACACCGCGTACGTCCTCTTCACCTCCGGC
TCCACCGGGCGGCCCAAGGGCGTGCCGCTCAGCCACGGCAACATCGCCCACTACTTCGAG
GTCCTCGACGCCCGCTACGACTTCACCGCGGACGACGTCTTCACCCAGACCTTCGACCTC
AACTTCGACTGCTCGCTCTTCGACCTGTTCTGCGCCTGGGGCGCCGGCGCGAGCGTCATC
CAGATCCCGCCCCAGGCATACCGGGACCTGCCGTCCCACCTCGCCGAACAGGGCGTCACC
GTCTGGTTCTCCACCCCGAGCAGCATCGCGCTGGTGCGCCGGCTCGGCGGTCTGGCACCC
GGCTCACTGCCCACCCTGCGCTGGAGCTTCTTCGCGGGCGAGGCACTCAAGTGCGCCGAC
ACGGAGGACTGGCAGCGCGCCGCGCCCGCCTCCTTCGTGGAGAACCTGTACGGCCCCACC
GAACTCACCGTCACCGTCACCGCCCACCGCTGGTCCCCGGAGGTCTCCCCGGTCGTCGGC
GCCAACGGCGTCGTCCCCATCGGCCCCCTCCACAAGGGCCTGGACCACGTCCTGATCGAC
GCCGGTGGACTGCCCCACCCCGACACCGGGGAACTCTGCGTCACCGGACCCCAGATGGCG
GGCCGCTACCTCGACCCCGCCGACGACCACGGCCGCTTCCTCGACCACGACGGGCGCCGC
TGGTACCGCACCGGGGACCGGGTCCGACTGGCCCCCGGCGGGGAACTCGTCTACCTCGGC
CGGATGGACGCCCAGGTCCAGATCCAGGGCTGGCGGGTGGAGCTCGCCGAGGTCGATCAC
GCCCTCCAGGGGTGCGAGGGCGTCGGCGAGGCCGTCACCGTCGGCGCCGCCACCGACGCG
GGCACCGAGCTGGTCGTCTTCTACACGGCCCCCGCCCCGGTGCCGCCCGTCCGCTTCGCC
GCCGTGCTGCGCGCCACCCTGCCCGACGGTGTCGTACCACGGCACTACCGCCATGTCGCC
GAACTGCCGTTGAATTCCAACCGCAAGATCGACCGGCGAGCACTGACCGCACGTGCCGAG
GAACTCCTCGGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000873 AMP-dependent synthetase/ligase (Domain) PF00501 AMP-binding IPR020845 AMP-binding, conserved site (Conserved_site) PS00455 AMP_BINDING
SignalP	No significant hit
TMHMM	No significant hit

Vicen_00160 Vicen_00180