Vicen_00150 Vicen_00150   Vicen_00170 Vicen_00170

Vicen_00160 : CDS information

close this sectionLocation

Organism
StrainHC34
Entry nameVicenistatin
Contig
Start / Stop / Direction41,987 / 42,235 / + [in whole cluster]
41,987 / 42,235 / + [in contig]
Location41987..42235 [in whole cluster]
41987..42235 [in contig]
TypeCDS
Length249 bp (82 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productpeptidyl carrier protein
Product (GenBank)acyl-carrier-protein
Gene
Gene (GenBank)vinL
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15112997] Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. (Chem Biol. , 2004)
[22010945] A natural protecting group strategy to carry an amino acid starter unit in the biosynthesis of macrolactam polyketide antibiotics. (J Am Chem Soc. , 2011)
[26831085] Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis. (Proc Natl Acad Sci U S A. , 2016)
comment
[PMID: 15112997](2004)
vicenistatin生合成gene clusterの同定論文。

VinL (82aa) : acyl carrier protein

配列解析からの機能推定。
starter unitに関連したaminoacyl carrier proteinとして機能すると考えられる。
starter unit 3-amino-2-methylpropionyl-VinLから、loading moduleのACPへとacyl基は移される。

---
[PMID: 22010945](2011)
VinJ, K, L, M, N, Oの酵素的解析を行っている論文。

放出されたphyrophosphateを検出するPhotometric assayにより、ATP-depenent ligase VinM and VinNの基質特異性を確認。

VinNは、3-methylaspartate(3-MeAsp)にのみ特異的に触媒する。また、そのmixtureにholo型VinL(ACP)を加えると、3-MeAsp-VinLが形成されることをLC-ESI-MSで確認している。

よって、VinNは3-MeAspを活性化し、VinLへの移動を触媒する。

---
[PMID: 26831085](2016)
VinKおよびVinK-VinL複合体の構造解析。

close this sectionPKS/NRPS Module

0
PCP2..59

close this sectionSequence

selected fasta
>peptidyl carrier protein [acyl-carrier-protein]
MWDAQFENLLRRYLPFLSADQPLEQDINLRDIGLDSLGTVELLSELENTYDVHFQDEALT
KETFETPGVLWKTLSQMVEPRH
selected fasta
>peptidyl carrier protein [acyl-carrier-protein]
ATGTGGGACGCCCAGTTCGAGAACCTCCTCCGCCGCTACCTCCCGTTCCTCTCCGCCGAC
CAGCCGCTGGAGCAGGACATCAACCTGCGTGACATCGGCCTCGACTCGCTCGGCACCGTC
GAGCTGCTCTCCGAGCTGGAGAACACCTACGACGTCCACTTCCAGGACGAGGCGCTCACC
AAGGAGACCTTCGAGACCCCCGGCGTGCTCTGGAAGACCCTCTCCCAGATGGTCGAGCCG
CGCCACTGA
[0] PCP2..59
[0] PCP4..177

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006162 Phosphopantetheine attachment site (PTM)
[31-46] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1-78] 1.59999889349252e-10 SSF47336
SSF47336   ACP_like
[8-58] 2.7e-07 PF00550
PF00550   PP-binding
[2-59] PS50075
PS50075   ACP_DOMAIN
[8-79] 5.8e-14 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
SignalP No significant hit
TMHMM No significant hit
Vicen_00150 Vicen_00150   Vicen_00170 Vicen_00170
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